... (Sandomenico et al., 2020). Figure 1: Comparison between the IgG antibody and scFv.   What...their lack of an Fc region. Disadvantages scFVs:  Compared to antibodies, scFvs tend to have lower affinities...intensity of a tagged protein. SunTag has two components: 1) a protein of interest that’s tagged with ....0000000000000145  Gąciarz A, Ruddock LW (2017) Complementarity determining regions and frameworks contribute...antib2020193  Khantasup K, Chantima W, Sangma C, Poomputsa K, Dharakul T (2015) Design and Generation of ...

...size, while SSC values depend on the structural complexity inside the cell or on its surface. Bear in mind...on the cell’s size, morphology, and structural complexity.   Height, Width, and Area Within FSC and...each coming with a different size and structural complexity. After several steps of tissue processing prior... for a single cell gate. However, make sure to compare H and A (or W and A).   Live/Dead Gating Having...cells stain positively for both markers. When comparing two markers simultaneously, we commonly describe...

...sword Microtubules are one of the most important components of the cytoskeleton. They provide cells with ...you’re interested in that other most-important component of the cytoskeleton – actin? Actin dynamics control...manipulating Rho GTPases based on BcLOV4. These single-component systems take advantage of BcLOV4’s light-induced...powerful enough, what about altering the chemical composition of the membrane itself? The Baskin Lab previously...K. H., & Chow, B. Y. (2022). Designing Single-Component Optogenetic Membrane Recruitment Systems: The ...

...for a Faculty Position" eBook Here 1. Have a competitive CV You don't necessarily need a Nature/Cell/Science... research is about and what you think you can accomplish. No matter how many times I say this, candidates...specific questions and experiments. Nobody EVER complains that a seminar was too easy to understand. Make...detail. Finally, state how successfully completing the research will advance the field, create a...yourself to me by email or in person. If you feel compelled to repay me in some way, I encourage you to help...

... a good story (1). From a deep sea shrimp to a compact powerhouse of bioluminescence, NLuc is 100-fold...yield, brightness and improved maturation time compared to similar orange fluorescent proteins. In their...more than 2-fold brighter signal from Antares as compared to Fluc in this model. The construct for Antares...transfer of energy from one fluorescent protein to a compatible fluorescent protein. FRET can be measured by ...fusion protein called CalFlux VNT. CalFlux VNT is composed of Venus fluorescent protein, the Troponin C Ca...

...lots of hustle and bustle, but the lab was very competitive and not very communal. That didn’t suit my collaborative...choosing a training lab, I strongly suggest you complete a work rotation in the lab if at all possible.... of effort, intimidating use of discipline or competence procedures Threats to personal standing – including...atmosphere makes you successful. Collaborative Competitive Papers have multiple authors One author plus...choose a grad school or postdoc lab. Having a comparison can be very helpful in uncovering strengths and...

...programs. The software has two components - a database of pre-compiled register designs and a search function...sites, promoters, genes, terminators, etc) are composed. Fear not – the state machine design space is ...circuits in general would benefit from a more comprehensive characterization of the recombinases at our ...the Lu Lab at MIT. He is broadly interested in biocomputing and biological circuits.     References 1. N...

...General Counsel at Atalanta, an early-stage biotech company that is developing RNAi therapeutics to treat neurodegenerative...law. He said, “you know, the patent lawyer at my company seems to really like what she does.” I thought ...patent counsel and ran a group at two fairly large companies, Genzyme and Biogen. They’re very different jobs...corporate work, and negotiating contracts with other companies. It's one of those "every day is something different...

..., in vitro screens cannot faithfully model the complex spatial, cellular, metabolic, and cytokine microenvironment... cells, an important immune cell type, in more complex and physiological microenvironments. These screens...immunogenic, a necessity for in vivo studies; (3) is compatible with single gene KO and pooled screening approaches...facilitate the isolation of modified cells; and (5) is compatible with the introduction of unique molecular identifiers...

...Beth Kenkel To complement our RNA Extraction Without A Kit blog post, we've compiled five methods for...methods to do this. 5. Plasmids 101: Plasmid Incompatibility by Leah Schiesow If you're thinking about ...features two mechanisms that cause plasmids to be incompatible. 4. RNA Extraction Without A Kit by Leah Schiesow...

...interviewed fellow Addgenies about their role in the company. One of the things I wanted to do was create content...were a part of the different departments of the company. I asked questions relating to their roles at Addgene...month, I can say with certainty that Addgene is a company that cares - not only in terms of its employees... Addgenies. I could take an active role in the company and not just feel like an additional person each...

...add another layer of complexity to imaging, as they allow one to monitor compartment/protein dynamics or... may not be appropriate for high-pH cellular compartments. Sattarzadeh et al. have shown that GFP can ...Drosophila, and mammalian cells. Photoconverted GFP is compatible with other FPs since its emission wavelength ...

...expansion, scientists will be able to request an AAV composed of an AAV plasmid in the Addgene repository and...by Addgene for legal approvals and technical compatibility with our production process. This process will...the service to be fulfilled and QC assays to be completed and reviewed. The timeline therefore will be as...follows:  Request review: 1–2 days Prep and QC completed: 6–8 weeks Average shipping time: 1–2 days (USA...

...recombination by KD, B2 and B3 resulted in complete or near-complete excision of the stop sequence. In contrast...toxicity, but with the caveat of partial rather than complete recombination. If you’re looking to use site-specific...this system. Gerald Rubin’s lab sought to make complex genome modifications in Drosophila using multiple...

... the clinic and cures. There is no doubt that companies are swarming to see what can be done with this...the insulin signaling cascades in only 4 months compared to a year or greater with older technologies. ... in the room. He offered the great analogy of comparing the CRISPR/Cas9 system to Norton Antivirus. Antivirus...the code of viruses to give information to the computer to fight the known viruses. Bacteria have figured...

...projects spanning from the identification of plant compounds and insecticidal proteins to the development of... reside within living plants and that produce compounds with various antimicrobial activities (1). She... newly identified proteins and enzymes. These compounds will later get tested for their potential agricultural...alternative source of essential omega-3 fatty acids complementing vegetable oil, a somewhat poor natural source...

...question is: how should the gRNAs be oriented in comparison to each other? The gRNAs must target different...different strands to create a DSB, but this can be accomplished with either a PAM-in or PAM-out orientation....preferences using HEK293 cells. They started by comparing D10A and H840A total editing efficiency in PAM-in...donor with 40 bp homology arms. The HDR efficiency compared favorably to using WT Cas9 with the left gRNA ...

...synthetize all compounds needed for growth, or auxotrophy, the inability to synthesize a compound needed to...an essential amino acid), and a plasmid that complements the auxotrophy (i.e., synthesizes the critical...yeast prototrophy kit containing 23 plasmids to complement auxotrophy. Negative selection: Cells that have...

...Until recently, there were no completely genetic tools that would allow researchers to label just a fraction...Sparse Predictive Activity through Recombinase Competition (SPARC), a genetic tool that allows for the labeling...PhiC31 irreversibly recombines one of the two competing attP sites to drive expression of either a stop...is placed downstream of the rest of the SPARC components and expression is controlled by the upstream ...

...some extra guidance to understand the specific components that make up a plasmid and why each is important...Drives transcription of the target gene. Vital component for expression vectors: determines which cell ...clone into. Regardless, once the cloning steps are complete, the vector containing the newly inserted gene...used in research or for therapeutics Addgene has compiled various educational resources to facilitate plasmid...