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Plasmid#170488PurposeSPELL Vav2(DH-PH-ZnF). Two components cloned into the same plasmid with T2A/P2A sequence. Component 2-T2A-P2A-Component 1DepositorInsertFRB, Vav2(C-lobe), iFKBP, Vav2(N-lobe), mCherry
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pOmpF
Plasmid#42606DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET30b_OmpAT7Sav
Plasmid#138589PurposeT7-tagged streptavidin with native C-terminus and periplasmic export signal OmpA; controlled by PT7 promoter; KanR, pBR322 oriDepositorInsertT7-tagged streptavidin variant with native C-terminus and OmpA signal peptide for periplasmic export
UseSynthetic BiologyTagsOmpA export signal and T7 tagExpressionBacterialMutationPromoterPT7Available sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
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OmpT
Plasmid#213137PurposeOmpT_native membrane expressionDepositorInsertOmpT
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pGreen_GUS_competitor
Plasmid#55208PurposeGUS-based microRNA target competitor used for testing function as target mimicryDepositorInsertbeta-glucuronidase
UsePlant expressionTagsExpressionMutationPromoterAvailable sinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCsy_complex
Plasmid#89232PurposePlasmid contains the genes Csy1, Csy2, Csy3, Csy4, cloned as a polycistronic operon from bacterium Pseudomonas aeruginosa PA14. Csy3 includes an N-terminal 6his tag and TEV protease cleavage siteDepositorInsertsCsy1
Csy2
Csy3
Csy4
UseTags6xHis-TEV proteaseExpressionMutationPromoterAvailable sinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJexpress414_OmpA171_WT
Plasmid#62646PurposeWild-type OmpA (N-terminal domain) with an HA tag in the 4th external loopDepositorInsertOmpA
UseSynthetic BiologyTags6His and FlAsHExpressionBacterialMutationPromoterT7Available sinceAug. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBIG2abc_hsHAUScomplex_FL
Plasmid#202203PurposeBaculoviral transfer vector to co-express 8 subunits of human augmin complexDepositorInsertsHAUS1 (HAUS1 codon-optimized form for expression in Trichoplusia ni cells, Human)
HAUS3 (HAUS3 codon-optimized form for expression in Trichoplusia ni cells, Human)
HAUS2 (HAUS2 codon-optimized form for expression in Trichoplusia ni cells, Human)
HAUS6 (HAUS6 codon-optimized form for expression in Trichoplusia ni cells, Human)
HAUS7 (HAUS7 codon-optimized form for expression in Trichoplusia ni cells, Human)
HAUS4 (HAUS4 codon-optimized form for expression in Trichoplusia ni cells, Human)
HAUS5 (HAUS5 codon-optimized form for expression in Trichoplusia ni cells, Human)
HAUS8 (HAUS8 codon-optimized form for expression in Trichoplusia ni cells, Human)
UseTags-mGFP-TEV-TwinStrep and His-tag (6x)ExpressionInsectMutationPromoterpolyhedrin promoterAvailable sinceSept. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pOmpT
Plasmid#112123PurposeBacterial expression outer membrane protein T (OmpT), wild typeDepositorInsertOmpT (ompT E. coli)
UseTagsExpressionBacterialMutationN-terminal signal sequence not included and repla…PromoterT7Available sinceNov. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOmpW
Plasmid#112121PurposeBacterial expression of outer membrane protein W (OmpW), wild typeDepositorInsertOmpW (ompW E. coli)
UseTagsExpressionBacterialMutationN-terminal signal sequence not included and repla…PromoterT7Available sinceNov. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJexpress414_OmpA171_H1237
Plasmid#64290PurposeOmpA redesign hybrid with wild-type strands 1237DepositorInsertOmpA_H1237
UseSynthetic BiologyTags6His and FlAsHExpressionBacterialMutationPromoterT7Available sinceMay 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJexpress414_OmpA171_H-3
Plasmid#64310PurposeOmpA redesign hybrid with wild-type strands 1245678DepositorInsertOmpA_H-3
UseSynthetic BiologyTags6His and FlAsHExpressionBacterialMutationPromoterT7Available sinceMay 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJexpress414_OmpA171_R4cons_G41A
Plasmid#64299PurposeConservative version of OmpA Redesign 4 with G41A mutationDepositorInsertOmpA_R4cons_G41A
UseSynthetic BiologyTags6His and FlAsHExpressionBacterialMutationPromoterT7Available sinceMay 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJexpress414_OmpA171_R4cons
Plasmid#62639PurposeConservative version of OmpA Redesign 4DepositorInsertOmpA_R4cons
UseSynthetic BiologyTags6His and FlAsHExpressionBacterialMutation25 mutations to lipid-facing surfacePromoterT7Available sinceMay 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJexpress414_OmpA171_R4cons_Y129H
Plasmid#64302PurposeConservative version of OmpA Redesign 4 with Y129H mutationDepositorInsertOmpA_R4cons_Y129H
UseSynthetic BiologyTags6His and FlAsHExpressionBacterialMutation25 mutations to lipid-facing surfacePromoterT7Available sinceAug. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJexpress414_OmpA171_H3478
Plasmid#64292PurposeOmpA redesign hybrid with wild-type strands 3478DepositorInsertOmpA_H3478
UseSynthetic BiologyTags6His and FlAsHExpressionBacterialMutationPromoterT7Available sinceAug. 6, 2015AvailabilityAcademic Institutions and Nonprofits only