We narrowed to 566 results for: PAC

1. Pushing the Limits of DNA Assembly

   Type

   Blog Post

   ...placed. With other techniques, when mutations are spaced farther apart, it becomes hard to accurately execute...

2. Visualizing Translation at the Single Molecule Level

   Type

   Blog Post

   ...s important to verify that these changes do not impact translation rate, as the authors of these papers...

3. Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

   Type

   Blog Post

   ... systems in Drosophila: A synthesis of time and space, Trends Genet. 20, 384–391 (2004). PubMed PMID: ...

4. Addgene Depositors Get More Citations

   Type

   Blog Post

   ...0.75   125.3 In figure 2, you can see the full impact from using these weighted numbers. Note: this does...

5. Plasmids 101: NGS Quality Control for Pooled Libraries

   Type

   Blog Post

   ...commonly uses a custom script derived from the count_spacers.py file from Feng Zhang’s laboratory. There are...

6. Virtual Science Conference Coming Up? Three Addgenies Share Their Experience

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   Blog Post

   ... an on-site conference, you really have to make space for it,” says Bachle. “On the flip side, you can...

7. 25 Work From Home Tips for Scientists

   Type

   Blog Post

   ... our mindfulness group is sticking with their Headspace practice even while working remotely. Social distancing...

8. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

   Type

   Blog Post

   ...type of antibodies from an Andean mammal called alpaca could neutralize the SARS-CoV-2 virus. But while...

9. Using CRISPR/Cas9 to Edit Disease Out of the Genome

   Type

   Blog Post

   ... difficult to use larger enzymes. To reduce the impact of off-target mutations, scientists are using sgRNA...

10. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type

    Blog Post

    ...beyond. These new iGluSnFR3 variants come in AAV packaged virus aliquots being deposited at Addgene. Other...

11. Four Factors that Differentiate the Stem Cell Field

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    Blog Post

    ...biology where questions of ethics can have profound impacts on the development of applications with the potential...

12. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type

    Blog Post

    ... Targeting three dCas9 molecules to a precisely spaced region allowed sequence replacement rates of approximately...

13. How to Lead a Great Meeting

    Type

    Blog Post

    ... include every person who could potentially be impacted by a project. Instead, invite the experts on the...

14. Advice for Scientists Starting a Lab

    Type

    Blog Post

    ... environment? Tom Ellis was offered a start-up package that included a postdoc and a PhD student in his...

15. Zebrafish Plasmid Collection

    Type

    Collection

    ...expression profiling during regeneration. Seurat - R package for analyzing single cell genomics in zebrafish...

16. CRISPR Plasmids - Mammalian Expression

    Type

    Collection

    ...change in RNA. dPspCas13b does not require a Protospacer Flanking Sequence (PFS), making it a very flexible...

17. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

    Type

    Blog Post

    ...The goal of this field is to functionally map the impact of cancer variants at scale. Check it out: Progress...

18. Top Requested Lentivirus and AAV of 2016

    Type

    Blog Post

    ...These viruses have been distributed in over 200 packages to more than 20 countries. With this initial success...

19. Why and how to start studying chromatin (with Addgene's help)

    Type

    Blog Post

    ...of a nucleus as “linear” is a misnomer. The DNA packed into our chromosomes is very much 3-dimensional...

20. Behind the scenes: Addgene’s new search engine and more

    Type

    Blog Post

    ...information, however, did not improve at the same pace as the quantity and quality of the information. ...