We narrowed to 733 results for: SON
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TypeBlog Post...these therapeutics. Jesse Bloom’s lab at the Fred Hutchinson Cancer Research Center developed a pooled plasmid...identified. New from the viral service By Jason Nasse We regularly add new viral aliquots from our...
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Chromoproteins: Colorful Proteins For Molecular Biology Experiments
TypeBlog Post...Why do these organisms have chromoproteins? One reason may be that chromoproteins protect corals from ...fluorescence lifetime imaging microscopy-based Förster resonance energy transfer (FLIM-FRET) in living cells (Figure... -
Uncovering Ancestral Bias in CRISPR Research
TypeBlog Post...science and society." Science 376, (2022): 250-252. Carlson J, Henn BM, Al-Hindi DR, Ramachandran S. Counter... Rachel Leeson... -
A Guide to Starting Your Own Journal Club
TypeBlog Post... figure by figure organization with a different person responsible for each figure. Others still are more... avenues including casual request via email, in person, or instant messaging but we as scientists often... -
Better Dyeing Through Chemistry & Small Molecule Fluorophores
TypeBlog Post...why should cell biologists talk to chemists? One reason is something called “the photon budget”: each sample...Protein Fusions Request Plasmids from the Michael Davidson Collection Find Fluorescent Protein Kits ... -
Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus
TypeBlog Post...opposed to four separate plasmids), there are a few reasons arguing against this approach. First, this would...PMCID: PMC3586226. 4. Mühlberger, Elke, et al. "Comparison of the transcription and replication strategies... -
The Importance of a Fun Workplace: Company Culture at Addgene
TypeBlog Post...philosophy of “work hard, play hard.” Part of the reason we sincerely like spending time together is that...work” all the time. She was a wise lady for many reasons, but this saying is especially true. If your event... -
To Codon Optimize or Not: That is the Question
TypeBlog Post...regulatory elements or use a non-canonical code (Gustafsson et al., 2004), such as Hoogsten base pairs and...Biol 13:30–34 . https://doi.org/10.1038/nsmb1031 Gustafsson C, Govindarajan S, Minshull J (2004) Codon bias... -
Hot Plasmids: Fall 2024
TypeBlog Post...Pk1] in a western blot assay. Image reused from Anderson et al., 2024 under CC BY license. Find ...Find Anti-V5 [IPI-SV5-Pk1] Antibody here! Anderson, Z., Zhu, H., Riedel, T., Moshinsky, D., Meijers, R... -
Antibodies 101: Reading a Flow Plot
TypeBlog Post...Langan, E., Siebert, S., Li, J., Greiner, D., & Anderson, D. (2015). Neutrophil Responses to Sterile Implant... Rachel Leeson... -
Viral Production at Addgene
TypeBlog Post...References Lock, M., Alvira, M. R., Chen, S.-J., & Wilson, J. M. (2014). Absolute determination of single-stranded...https://doi.org/10.1089/hgtb.2013.131 Wang, Y., Bergelson, S., & Feschenko, M. (2018). Determination of ... -
Hot Plasmids Spring 2024
TypeBlog Post...proteins, and modifications to the pegRNA. The Britt Adamson Lab recently used a CRISPRi screen to identify ...useful materials for research into Alzheimer’s, Parkinson’s, ALS, and Huntington’s Disease, including plasmids... -
Tips for Writing a Good Cover Letter
TypeBlog Post... Quick tips for writing a good cover letter Personalize your cover letter to the job and job requirements...Soriano-Carot in September 2016 and was updated by Rachel Leeson in May 2024. Additional Resources on the Addgene... -
Lab to Office Culture Shock
TypeBlog Post... and for a very brief period of time, the only, person to know a brand new fact is often enough to keep...learning to “speak science,” and virtually every person you talked to during the day was also a scientist... -
How to Write a Scientific Review Article
TypeBlog Post...becoming an expert in your field and are the perfect person to be writing the review! Doing so is a great way...Good luck! References 1. Haery, Leila, Ryan C. Thompson, and Thomas D. Gilmore. "Histone acetyltransferases... -
The Challenges of Cell Culture
TypeBlog Post...of false interpretation in cell culture for this reason. Unreproducible results are rife. Misidentified...PMID: 12001993. 4. Lucey, Brendan P., Walter A. Nelson-Rees, and Grover M. Hutchins. "Henrietta Lacks,... -
Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs
TypeBlog Post...consider using moxFPs over standard FPs for the reasons listed above. In addition, because the moxFPs are...11736655. Pubmed Central PMCID: PMC1222268. 7. Aronson, Deborah E., Lindsey M. Costantini, and Erik L.... -
Important Considerations in Optogenetics Behavioral Experiments
TypeBlog Post...summarized in a short blog post, but some examples I’m personally interested in include: intracranial self-stimulation... to proceed with your behavioral experiments. Comparison of c-fos amongst animals that receive different... -
Plasmids 101: E. coli Strains for Protein Expression
TypeBlog Post...act on DNA, or are enzymatically active. For this reason, recombinant proteins are typically expressed in...culture from reaching the desired density within a reasonable time-frame. For these cases, some strains carry... -
Harnessing Bacterial Toxins for Allelic Exchange
TypeBlog Post...arguably the most widely and productively used (Thomason et al., 2014). In recent years, CRISPR-Cas9 technology...reports 5 (2015): 15096. PubMed PMID: 28060411. Thomason, Lynn C., et al. "Recombineering: genetic engineering...