Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 718 results for: STO

Showing: 441 - 460 of 718 results

  1. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ...pUC multimerization was reduced by growing seed stocks at 30°C compared to 42°C. Screen multiple colonies...platform incorporating low metabolic burden seed-stock and fed-batch fermentation processes. Biotechnol...

  6. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ...generated the first optically active saCas9 protein. Stop light! Photoactive anti-CRISPRs halt Cas9 activity... which would add the PCK upon reaching the amber stop codon, TAG. (To learn more about site-specific incorporation...

  7. Antibody Guide

    Type
    Guide
    ...antibodies can be stored at 4 °C for short-term storage. For long-term storage they should be stored at -20 °C... Antibody Handling Storage Check the manufacturer’s instructions for exact storage requirements. As a ...tips for storing your antibody: Antibodies conjugated to fluorophores should never be stored below 4 °...define key terms, summarize antibody production and storage techniques, and explain common applications. Science...complete. HRP-conjugated antibodies should not be stored in sodium azide, as it acts as an inhibitor to ...location. Includes: Immunofluorescence (IF) Immunohistochemistry (IHC) Immunocytochemistry (ICC) Cell Sorting...in these assays. This method is suitable for DNA:histone modifier interactions, which have strong binding...

  8. Molecular Biology Reference

    Type
    Guide
    ...2 0. Addgene recommends making 1000X stock solutions and storing aliquots at -20°C. To use, dilute your...media, add 100 μL of a 100 mg/mL ampicillin stock (1000X stock) to 100 mL of LB. DNA sequencing for plasmid...bacterial cells that have been preserved as glycerol stocks. Functional in many species and can be useful for...synthesized RNA signals for the transcription process to stop. An expression vector can also include an enhancer...media or LB agar plates. Antibiotic Recommended Stock Concentration Recommended Working Concentration ...place of ampicillin. Preparing Antibiotics Create a stock solution of your antibiotic. Unless otherwise indicated... acid. There are 20 amino acids plus a start and stop codon. Below you will find helpful resource tables...

  9. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...transport in neurons. (b) Genetic cargo can be customized to control transgene expression. Gene regulatory...Chuapoco, M. R., Flytzanis, N. C., Goeden, N., Christopher Octeau, J., Roxas, K. M., Chan, K. Y., Scherrer...

  10. Lentiviral Vector Guide

    Type
    Guide
    ...VSV-G due to wide infectivity) VSV-G (vesicular stomatitis virus G protein) is the most common envelope ...supernatant containing the viral vectors is removed and stored or centrifuged to concentrate. You can use crude...line protocol . Browse Addgene's collection of in-stock lentiviral preps . CRISPR Genomic Screens Genome-wide...on addgene.org Lentiviral Plasmids Collection In-Stock Lentiviral Preps Addgene's Viral Prep Service Viral... The viral envelope gene; typically vesicular stomatitis virus G glycoprotein (VSV-G), an envelope protein...

  13. 2022 Depositor Week

    Type
    Blog Post
    ... of Fame  Thursday: 8 Great Depositor Research Stories   Friday: Specialized Depositor Shout-Out  We'll...

  14. Addgene's 20th Anniversary Party

    Type
    Blog Post
    ...without recognizing our incredible depositors and requestors. Thank you all so much for an amazing twenty ...

  15. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    ... today, we’ll start with a CRISPR overview and history. Tuesday will focus on generating knockouts using...

  16. Chemogenetics Guide

    Type
    Guide
    ...phospholipase C, which releases intracellular calcium stores. There are currently three Gq DREADDs based on ... new window) Chen X, Choo H, Huang X-P, Yang X, Stone O, Roth BL, Jin J (2015). The First Structure–Activity...46. PMID: 25937170 (Link opens in a new window) Weston M, Kaserer T, Wu A, Mouravlev A, Carpenter JC, ... Stem Cell-Derived Neurons Repair Circuits and Restore Neural Function. Cell Stem Cell. 28:112-126.e6....

  17. We’re Going Viral This Week!

    Type
    Blog Post
    ...we’ll be at SfN2019. If you’re attending SfN2019, stop by booth #2163 to say hi and learn more about Addgene...
Showing: 441 - 460 of 718 results