Multiplex Genome Editing with CRISPR-Cpf1
Type
Blog Post
...copy of the DNA sequence, which is a suitable substrate for Cpf1. This means Cpf1 can bind and cleave ...
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A Novice's Perspective on Neuroscience - SfN2017
TypeBlog Post...Neuroscience Research Neuroscience covers many subfields When I first began looking into the details of... -
Viral Vectors 101: The AAV Retrograde Serotype
TypeBlog Post... spinal cord (Wang, 2018, Metcalfe, 2022) and substantia nigra (Tervo, 2016). Discrepancies in transduction... -
Viral Vectors 101: Viruses as Biological Tools
TypeBlog Post...feature makes them an attractive tool for human subject delivery, as immunogenicity was previously a large... -
Sharing is Caring: But How to Distribute Open Hardware?
TypeBlog Post...assembly, the feasibility of manufacturing, and subsequent assembly can be difficult to overcome. Universities... -
Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors
TypeBlog Post...about Addgene in our "Inside Addgene" blog series Subscribe to the Addgene blog Resources on Addgene.org ... -
15 Years of Addgene: The Top 15 Plasmids
TypeBlog Post...in the original publication but its name was subsequently changed to clarify that it does not contain ... -
How to Keep a Lab Notebook for Bioinformatic Analyses
TypeBlog Post...experiment 1. Use camelCase: In camelCase, each subsequent word in a file name after the first word begins... -
Antibodies 101: Fab Fragments
TypeBlog Post...flexibility of and demand for Fab fragments and subsequently Fab fragments intended for use as primary antibodies... -
It’s Bioelectric! An Exciting Interdisciplinary Field of Research
TypeBlog Post...during regeneration, the worm creates two heads. Subsequent cutting results in a two-headed worm, despite... -
Troubleshooting Your Plasmid Cloning Experiment
TypeBlog Post...hopefully obtain your coveted plasmid with no substantial delays. Design, design, design Before diving ... -
Plasmid Tools for Microbiome Studies
TypeBlog Post...to stop the production of new particles. With subsequent cell divisions, the fraction of cells containing... -
Supporting Rare Disease Research with the 2020 BeHEARD Awards
TypeBlog Post...these parts, the GNB1 gene, which codes for G𝛃1 subunits of G protein complexes, lead to a rare type of... -
Antibodies 101: Selecting the Right Antibody
TypeBlog Post...antibody that is directly conjugated to a detection substrate, such as a fluorophore, or a conjugated secondary... -
What's New in CRISPR - November 2021
TypeBlog Post...read more content related to CRISPR technology, subscribe to our CRISPR blog content. In this post we ... -
Lighting Up Cell Signaling with Photoswitchable Kinases
TypeBlog Post...few such methods exist and they only work for a subset of kinases. The Problem: There is no common method... -
RANbodies: Reporter Nanobody Fusions
TypeBlog Post...detection RANbody is detected enzymatically with HRP substrate More sensitive than detecting antigen with a primary... -
Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute
TypeBlog Post...Twitter @sejkeown. If you enjoyed this series, subscribe to the Career Channel of the blog so you don’t... -
Antibodies 101: Conjugation
TypeBlog Post... (HRP) and Alkaline Phosphatase (AP). Various substrates for each of these enzymes allow you to detect... -
Antibodies 101: Polyclonal Antibodies
TypeBlog Post...protein G each have different affinities for IgG subtypes from different animals so it’s important to choose...