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  1. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

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    Blog Post
    ...activators The simplest of the dCas9 activators consists of dCas9 fused to a single transcriptional activation...Mediator complex, generally the strongest activator, consists of a dCas9-VP64 fusion and a modified gRNA that...

  2. Deciphering the Mysteries of Behavior with Viral Vectors

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    Blog Post
    ... (BDNF). BDNF is a small protein involved in neurogenesis, neuronal survival, and synaptic plasticity....Groves, J. O. "Is it time to reassess the BDNF hypothesis of depression?." Molecular psychiatry 12.12 (...

  3. Designing Your Chalk Talk for the Academic Job Interview

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    Blog Post
    ...not need to be grant aims. Each aim could be the basis of a single grant. Either way, think realistically...tools? A novel dataset? Unique expertise? A novel hypothesis? Part of the motivation for doing the research...

  5. Plasmids 101: Codon usage bias

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    Blog Post
    ...alternative tRNAs Codon optimization With low cost DNA synthesis, one of the primary ways researchers solve the...created codon optimization algorithms and DNA synthesis companies such as IDT and GenScript host online...

  6. Transferable Skills Guide: Creativity

    Type
    Blog Post
    ... well received, understand that people can be resistant to change. Ask your peers or your superiors to...to explain their rationale when they’re resistant to new ideas. If their answer is “we’ve always done ...

  7. AAV Titers: Where Do They Come From And What Do They Mean?

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    Blog Post
    ...image shows that the vast majority of the vectors consist of full particles (white arrowhead) relative to...Droplet Digital PCR versus qPCR for gene expression analysis with low abundant targets: from variable nonsense...

  8. CRISPR Activation: A Practical Guide

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    Blog Post
    ...be made easily and cheaply using oligo library synthesis. Prior to Cas9 activators, similar tools were ...function (Pan et al., 2018). cDNA libraries, which consist of plasmids that over-express coding sequences ...

  9. Tips for CRISPR Gene Editing in Mice

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    Blog Post
    ...content.      References 1. Cho, Seung Woo, et al. "Analysis of off-target effects of CRISPR/Cas-derived RNA-guided... et al. "Feasibility for a large scale mouse mutagenesis by injecting CRISPR/Cas plasmid into zygotes....

  10. The Challenges of Cell Culture

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    Blog Post
    ...provenance (this is the history of the cell line consisting of records of the origin and propagation of the...Transition Is Required for Acquisition of Anoikis Resistance and Metastatic Potential in Adenoid Cystic Carcinoma...

  12. An Integrin Antibody Toolkit from IPI

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    Blog Post
    ... transmitted through the integrin, where it is resisted by the ligand embedded in the extracellular environment...development, extended-open integrins enable tissue morphogenesis, organ development, and the shaping of the extracellular...

  14. Adeno-associated Viruses (AAVs) for Genome Editing

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    Blog Post
    ...modification of interest, ordering them from a gene synthesis company such as IDT or Genscript, and cloning ...using adeno-associated virus (AAV)." Chromosomal Mutagenesis. Humana Press, New York, NY, 2015. 75-103. PubMed...

  15. Hot Biosensors 2022: Year-End Roundup

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    Blog Post
    ... et al. 2022). Lactate is a key metabolite in glycolysis, so this sensor will be a valuable tool to study...lifetime imaging. B) LiLac responses are highly resistant to pH changes. Image adapted from Koveal et al...

  16. Five Popular Model Organisms

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    Blog Post
    ... set of mutations that made cancer cells more resistant to chemotherapy. You can find these plasmids at.... Thus yeast can be used to study the molecular basis of human diseases that are caused by a specific ...

  17. How to Negotiate a Successful Lab Start Up Budget

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    Blog Post
    ... steps: 1. Define your timeline Start with your thesis in mind. Imagine the kind of data your dream discovery...consultant and coach in Boston, MA. He specializes in assisting research scientists create productive and manageable...

  19. Viral Vectors 101: AAV Variables That Matter

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    Blog Post
    ...Aschauer, D. F., Kreuz, S., & Rumpel, S. (2013). Analysis of Transduction Efficiency, Tropism and Axonal...Hosokawa, Y., & Konishi, H. (2014). A comparative analysis of constitutive promoters located in adeno-associated...

  20. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ...generate more signal from low abundance targets. Analysis/Presentation Once you’ve captured your images,...imaging approach for deep phenotyping and spatial analysis of cells in complex tissues. Proceedings of the...
Showing: 441 - 460 of 729 results