We narrowed to 738 results for: Sis

... a hygromycin resistance gene as the plant-selectable marker and a kanamycin resistance gene for bacterial...

...however, the protein integration host factor (IHF) assists with the process: it bends the DNA to redistribute...with a beneficial function (usually antibiotic resistance).  In the lab, low copy plasmids may result in...

... (BDNF). BDNF is a small protein involved in neurogenesis, neuronal survival, and synaptic plasticity....Groves, J. O. "Is it time to reassess the BDNF hypothesis of depression?." Molecular psychiatry 12.12 (...

...not need to be grant aims. Each aim could be the basis of a single grant. Either way, think realistically...tools? A novel dataset? Unique expertise? A novel hypothesis? Part of the motivation for doing the research...

...Susanna Stroik The Liu lab recently used phage-assisted continuous evolution and protein engineering to...  Doman, J.L., Pandey, S., et al. (2023). Phage-assisted evolution and protein engineering yield compact...

...screening, followed by sequencing and further data analysis. Based on Figure 31.5.2 from McDade et al., 2016...scripts provided by the depositing lab for data analysis.  I'm often asked whether this step can be skipped...

...the hippocampus. This technology has formed the basis of the Rett syndrome program being pursued at Vico...nature24058 Tillotson R, Bird A (2020) The Molecular Basis of MeCP2 Function in the Brain. Journal of Molecular...

...activators The simplest of the dCas9 activators consists of dCas9 fused to a single transcriptional activation...Mediator complex, generally the strongest activator, consists of a dCas9-VP64 fusion and a modified gRNA that...

... well received, understand that people can be resistant to change. Ask your peers or your superiors to...to explain their rationale when they’re resistant to new ideas. If their answer is “we’ve always done ...

...be made easily and cheaply using oligo library synthesis. Prior to Cas9 activators, similar tools were ...function (Pan et al., 2018). cDNA libraries, which consist of plasmids that over-express coding sequences ...

...content.      References 1. Cho, Seung Woo, et al. "Analysis of off-target effects of CRISPR/Cas-derived RNA-guided... et al. "Feasibility for a large scale mouse mutagenesis by injecting CRISPR/Cas plasmid into zygotes....

...provenance (this is the history of the cell line consisting of records of the origin and propagation of the...Transition Is Required for Acquisition of Anoikis Resistance and Metastatic Potential in Adenoid Cystic Carcinoma...

...occasionally beaten to press, as there is so much analysis possible. Most scientists are, however, privileged...requirements and likely will not do so (calling it a “sisyphean task” given the endless types of data). Rather...

...modification of interest, ordering them from a gene synthesis company such as IDT or Genscript, and cloning ...using adeno-associated virus (AAV)." Chromosomal Mutagenesis. Humana Press, New York, NY, 2015. 75-103. PubMed...

...Wilson, C., Doman, J. L., & Liu, D. R. (2019). Analysis and minimization of cellular RNA editing by DNA... E., Doudna, J. A., & Liu, D. R. (2020). Phage-assisted evolution of an adenine base editor with improved...

..., Y., & Peng, C. (2018). Overview of MicroRNA biogenesis, mechanisms of actions, and circulation. Frontiers...Ørom, U. a. V. (2023). Recent progress in miRNA biogenesis and decay. RNA Biology, 21(1), 36–43. https:/...

... set of mutations that made cancer cells more resistant to chemotherapy. You can find these plasmids at.... Thus yeast can be used to study the molecular basis of human diseases that are caused by a specific ...

...physical structure, separate chromosomes during mitosis, support transport of organelles and vesicles, ...the samurai sword called a katana. Opto-katanin consists of two separate blue light-sensitive systems, ...

... et al. 2022). Lactate is a key metabolite in glycolysis, so this sensor will be a valuable tool to study...lifetime imaging. B) LiLac responses are highly resistant to pH changes. Image adapted from Koveal et al...

... transmitted through the integrin, where it is resisted by the ligand embedded in the extracellular environment...development, extended-open integrins enable tissue morphogenesis, organ development, and the shaping of the extracellular...