We narrowed to 617 results for: TIL

1. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

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   Blog Post

   ...repair that normally accompanies Cas9 cutting. We still don’t know the mechanism underlying dCas9 editing...

2. Synthetic Photobiology: Optogenetics for E. coli

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   Blog Post

   ...condensed the system to two streamlined plasmids. The utility of these TCSs was limited by a few factors. These...

3. Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

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   Blog Post

   ...samples of similar size. Run the gel at 80-150V until you have good separation between your bands. Stopping...

4. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

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   Blog Post

   ... also created. The depositors demonstrated the utility of light-mediated suppression of synaptic transmission...

5. Luminescent Imaging with Nano-lanterns

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   Blog Post

   ...based on Nano-lantern, showing the technique’s versatility and adaptability to many different contexts. ...

6. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

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   Blog Post

   ... many tricks up their sleeves and most patients still don’t benefit from this approach. One central bottleneck...

7. A Quick Guide to a Career in Software Product Management

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   Blog Post

   ...they may need to help improve their work. Then, I distill and translate that valuable information back to...

8. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

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   Blog Post

   ... of a T7 RNA polymerase and cannot be expressed until the base editor corrects early stop codons within...

9. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

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   Blog Post

   ... can be difficult to replicate. Unclear methods Until someone attempts to replicate the results of another...

10. Screening for Successful Genome Editing with Digital PCR

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    Blog Post

    ...although digital PCR is a great screening tool, it still requires target DNA sequencing to confirm the exact...

11. Hot Plasmids - May 2022

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    Blog Post

    ...culture and whole organisms (see our 2019 overview). Until recently, the approach has been hampered by the ...

12. AAV Q&A with Tim Miles

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    Blog Post

    ...questions via text! (well, maybe not all of them. We still haven’t gotten to the bottom of why hot dogs are...

13. Plasmids 101: Inducible Promoters

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    Blog Post

    ... inducible tetracycline ON (Tet-On) system, a versatile tool developed for use in prokaryotes and eukaryotes...

14. A Novice's Perspective on Neuroscience - SfN2017

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    Blog Post

    ...interested in using AAVs because they provide a highly versatile means of delivering genes to many different cell...

15. Targeted Mutagenesis with EvolvR

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    Blog Post

    ...loci in bacteria. Its modular nature provides versatility in defining the mutational rate and editing window...

16. 15 Years of Addgene: The Top 15 Plasmids

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    Blog Post

    ... Their long-lasting popularity illustrates the utility these tools have in many different areas of biology...

17. Early Career Researcher Toolbox: Social Media for Scientists

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    Blog Post

    ...using them to my professional advantage. It wasn’t until I wrote a blog post about a research paper that ...

18. From Lab Bench to Leadership: Transitioning into Management as a PhD

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    Blog Post

    ... "do it yourself" can result in burnout and underutilized team potential. I often thought that performing...

19. Flow Cytometry Readouts: Yes, No, and Everything in Between

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    Blog Post

    ...that shifts to the left. When using MFI, you can still continue gating populations of interest with normal...

20. Viral Vectors 101: Gamma-Retroviral Packaging Systems

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    Blog Post

    ...using helper-free packaging cell lines This method utilizes a packaging cell line that stably expresses Gag-Pol...