We narrowed to 630 results for: TIL;

1. Synthetic Photobiology: Optogenetics for E. coli

   Type

   Blog Post

   ...condensed the system to two streamlined plasmids. The utility of these TCSs was limited by a few factors. These...

2. Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

   Type

   Blog Post

   ...samples of similar size. Run the gel at 80-150V until you have good separation between your bands. Stopping...

3. Hot Plasmids - May 2022

   Type

   Blog Post

   ...culture and whole organisms (see our 2019 overview). Until recently, the approach has been hampered by the ...

4. AAV Q&A with Tim Miles

   Type

   Blog Post

   ...questions via text! (well, maybe not all of them. We still haven’t gotten to the bottom of why hot dogs are...

5. From Lab Bench to Leadership: Transitioning into Management as a PhD

   Type

   Blog Post

   ... "do it yourself" can result in burnout and underutilized team potential. I often thought that performing...

6. Flow Cytometry Readouts: Yes, No, and Everything in Between

   Type

   Blog Post

   ...that shifts to the left. When using MFI, you can still continue gating populations of interest with normal...

7. Viral Vectors 101: Gamma-Retroviral Packaging Systems

   Type

   Blog Post

   ...using helper-free packaging cell lines This method utilizes a packaging cell line that stably expresses Gag-Pol...

8. A Quick Guide to a Career in Software Product Management

   Type

   Blog Post

   ...they may need to help improve their work. Then, I distill and translate that valuable information back to...

9. Clear and Accessible Writing

   Type

   Blog Post

   ...supernatant migrates through increasingly dense layers until the macromolecule density is equal to the buoyant...

10. CasPEDIA: A Functional Classification of Cas Enzymes

    Type

    Blog Post

    ... However, many situations could arise where you still need more information. If you are performing a viral...

11. Viral Vectors 101: Inverted Terminal Repeats

    Type

    Blog Post

    ... efficiency. Our practical knowledge of ITRs is still limited, but this area of research is important ...

12. Viral Vectors 101: Viral Vector Elements

    Type

    Blog Post

    ...into the host genome (more on this below), it’s still used to deliver gene expression constructs. Sandwiched...

13. Viral Vectors 101: Biosensors

    Type

    Blog Post

    ...you covered with viral resources and safety tips. Still worried? We make virus with some of our most popular...

14. Viral Vectors 101: Viral Applications

    Type

    Blog Post

    ...Additional Resources on the Addgene Blog AAV: A Versatile Viral tool for Gene Expression in Mammals Listen...

15. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

    Type

    Blog Post

    ...similar cleavage products even when guides were tiled across a target RNA, indicating that this enzyme...

16. Plasmids 101: Inducible Promoters

    Type

    Blog Post

    ... inducible tetracycline ON (Tet-On) system, a versatile tool developed for use in prokaryotes and eukaryotes...

17. A Novice's Perspective on Neuroscience - SfN2017

    Type

    Blog Post

    ...interested in using AAVs because they provide a highly versatile means of delivering genes to many different cell...

18. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

    Type

    Blog Post

    ... also created. The depositors demonstrated the utility of light-mediated suppression of synaptic transmission...

19. Targeted Mutagenesis with EvolvR

    Type

    Blog Post

    ...loci in bacteria. Its modular nature provides versatility in defining the mutational rate and editing window...

20. 15 Years of Addgene: The Top 15 Plasmids

    Type

    Blog Post

    ... Their long-lasting popularity illustrates the utility these tools have in many different areas of biology...