We narrowed to 844 results for: Tat

... Nucleic acid based machines Recombinase-based State Machines Enable Order-dependent Logic in vivo Nathaniel...received by a cell. These so-called recombinase-based state machines could potentially be used to report upon...this post to learn more about recombinase-based state machines and how they function. Adapting Toehold...

...journal will help a Mentee track progress, adjust expectations, and record milestones in reaching goals. The...that I try to exercise every morning, but the temptation to skip the gym is strong. There are a few women...with regular accountability checks. Focus on implementation of your goals and respect the time your Mentor...

...Now with the DAP array, it is possible to use the state-of-art base editor, prime editor, or any CRISPR-...CRISPR-based gene editors, for correcting mutations or installing functional genes to study and potentially...).   Fig. 2: Comparison of previous representative dual-gRNA architectures with DAP array ...

...  The iodine-modifications also shift MG-2I’s excitation wavelength to 666 nm, which is within the near-infrared...possible to do things like in vivo imaging.   Excitation with NIR light also produces less non-specific... non-specific ROS when exposed to YFP and RFP excitation wavelengths, which suggests that it could be ...

... do with them. In some cases, there might be a mutation that disrupts the function of YFG. You could compare... compare this mutant fly to one lacking the mutation in the same genetic background. In other cases, YFG... knock down gene expression, rescue knock out mutations, and overexpress viral genes. Additionally, other...

...Manager Patent Lawyer Science Market Research Statistician   Attention to detail Patience, accuracy ...Computer and writing skills Writing a thesis or dissertation demonstrates that you have advanced writing ...including: Science Reporter Technical Writer Statistician Bioinformatician Science Writer Grant Writer...

...elements often determines the type of plasmid and dictates how it might be used in the lab. Regardless of... Addgene Resource(s) Cloning Plasmids Used to facilitate the cloning of DNA fragments. Cloning vectors... gene function as well as identify changes or mutations that may cause certain diseases. Sequencing DNA...

.... While certain cell types, such as principal excitatory cells and glia, have been successfully targeted...researcher’s locus of interest. NHEJ- or HDR-induced mutations in ATP1A1 can render cells resistant to the small...improves editing efficiency. Importantly, the point mutations created by Agudelo et al. prevent ouabain binding...DNA molecular weight markers The Tan lab at Penn State - set out to “rule” Molecular Biology. Under the...

...than indirect ones and can be used in highly quantitative assays. On the other hand, they are not as sensitive...stability Limited protein size range Tris-acetate 40–500 kDa Tris-tricine 150 V, 1–3 hours ...detection. Film also needs to be digitized for quantitative work — and with the advent of online lab notebooks...high-abundance single protein controls in semi-quantitative immunoblotting. Journal of Neuroscience Methods...

...this one mutated methionine was not enough to prevent alternative initiation, so they mutated the methionines...the target site (the same sequence you’d like to mutate in the genome) and an out-of-frame H2B (histone...

...long sequences with reduced risks of unwanted mutations in mammalian cells. Long sequences traditionally...traditionally have higher risks of off-target mutations. These plasmids contain catalytically inactive dCas9 and...challenge due to multiple factors, including limitations of number of flourescent proteins that can be...

...Chromatin immunoprecipitation (ChIP) is an extremely useful technique that provides insight into protein...to counteract the effects of increased sample agitation; a shorter sonication time could be an opportunity...resources on Addgene’s blog Antibodies 101: Immunoprecipitation  Antibodies 101: Polyclonal Antibodies Antibodies...

...Cas12a for multiplexed gene perturbation The Penn State Protein Ladder Engineered miniature CRISPR-Cas system...2021. doi: 10.1038/s41587-020-0600-6. The Penn State Protein Ladder  by: Rachel Leeson This simple, inexpensive...expression and purification. Figure 3: The Penn State Ladder run on SDS-Page gels. A: individually expressed...

...megabase away, or looking at the histone methylation status at a double-strand break that needs repairing. ...start sharing your plasmids through Addgene, don’t hesitate to start the process! Addgene depositors who study...linked below are partially curated from the presentations and posters I saw at the 2018 Keystone Chromosome...

...promoters, which can be switched from an OFF to an ON state, and how you might use these in your research. When...negative control. Positive inducible In the OFF state, the promoter is inactive because the activator ...transcription.     Negative inducible In the OFF state, the promoter is inactive because a bound repressor...

...to neuroscience prior to this conference was a rotation in Qiao Zhou’s lab in graduate school where I ...neuroscience and you don’t have a background in statistics and programming, you’d be wise to start working...and brain monitors. Walking around the poster presentation area, I honed in on projects using biological...

... efficient. Pro-Tip Make sure to reverse the orientation of the crRNA’s direct repeats when expressing...preventing packaging of the virus.  Reversing the orientation of the direct repeat protects the (+) stranded...cells, but notice how Zetsche et al reverses the orientation of the promoter driving expression of the crRNA...

...there is a classification system, CasID, to help facilitate direct comparison of different enzymes. CasPEDIA... their sequence (NGG, etc.) as well as their orientation relative to the gRNA (5’ or 3’). In some cases...would be helpful to know for viral packaging limitations. Maybe you want to read literature on the less...

...proteins. On the other hand, they don’t require an excitation light source (usually a laser in fluorescence...to a nearby fluorophore, which emits light. No excitation illumination needed — just the luminescent substrate... proteins to be visualized simultaneously. Complementation systems for examining protein-protein interactions...

...to overexpress endogenous RNAi pathways. These mutations however can interfere with other genetic pathways...enzymes that overcome both the speed and toxicity limitations of APEX2 and BioID. In the Ting Lab’s recent ...proteome contains a breadth of modular domains that facilitate protein-protein interactions and mediate biochemical...