We narrowed to 512 results for: Trac;

... every piece of work you touch, and be a more attractive candidate for that ideal job. How can you identify...

... knob domain, and hexon), as well as other intracellularly-exposed proteins and downstream cellular events...

...the stress of selection you can clip a leaf and extract genomic DNA for genotyping. This won’t hurt your...

...500 bp to 1 kb range, however, shorter homology tracts have recently been used with some success (Yu et...

... quickly – modifying the length a polyhistidine tract does not greatly alter the size of the tag.   Table...

...functions in the hope of developing drugs against intractable diseases. Currently, a number of labs are using...

...screening with CRISPR and Lentivirus Read about the ClonTracr Library Resources on Addgene.org Addgene's most...

...data for our DNA-based reagents. Not only do we extract as much information as possible from depositing...

...strengths.    . Steps of an IP: 1) Protein extracts from cells or tissues contain a complex mix of...

... plasmids for multicopy integration. (B) Anhydrotetracycline (aTc) induces expression transposases leading...

...When choosing a Cas9, remember to check that your tracrRNA and crRNA (or synthetic gRNA) are derived from...

...people within similar fields (ASM Microbe has eight tracks), something we didn’t have enough people to do ...

...Addgenies, development includes everything from tracking plasmids from the moment a deposit kit is sent...

...Confirmation Pick 2–3 positive colonies, grow them, and extract plasmids. Confirm the correct guide insertion by...

...the terminating nucleotide and records it in a “trace” file to determine the sequence. Figure 1...

...Glossary Here’s a quick refresher if you ever lose track of which acronym is which. Acronym Full name ...

...with Cas9 on an integrative plasmid containing tetracycline repressor TetR and resistance selection marker...

...being clear about what was expected, and keeping track of speakers. During your graduate or postdoctoral...

...., Pons, J. A., & Muro, M. (2018). In vitro intracellular IFNγ, IL-17 and IL-10 producing T cells correlates...

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