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  2. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ...has occurred. Although the original Y2H systems utilized the yeast Gal4 activator, bacterial LexA DBD and...bait and prey protein fusions to demonstrate the utility of the Y2H system, it is more common to use many...

  5. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    ... developed, though, researchers at HuidaGene Therapeutics guessed that fusing MPG to an ABE would allow...recognize T and/or C substrates. The HuidaGene Therapeutics team has deposited their editors, called gTBE...

  6. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    ... spatiotemporal control over its expression. Constitutive or inducible Cre recombinases can be introduced...method to dissect the individual and shared contributions of transcript isoforms to neuronal physiology...

  8. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ... processing in live cells. Indeed, groups have utilized dCas13 fusion proteins for imaging, tracking, ...global RNA expression, cell stress, and RNA size distribution. When comparing LwaCas13a to shRNA, they found...

  10. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    ...generation of base modifiers available. This system utilizes an RNA template containing the desired edit adjoined...like with base editing, alternative Cas9s can be utilized allowing for PAM flexibility. Fig. 2: Prime...

  12. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ...Cas9. Unfortunately, certain drawbacks limit the utility of these systems: St1Cas9 requires a very specific...  Wang D, Zhang F, Gao G (2020) CRISPR-Based Therapeutic Genome Editing: Strategies and In Vivo Delivery...

  14. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ...pathogen or virus detection), therapeutics and sensitive genotyping. The beauty of SHERLOCK systems is that...bottleneck. In the last few years, scientists have utilized CRISPR-Cas9 protein variants, Cas13, and Cas12a...

  15. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    ...molecules. Using a technique called systematic evolution of ligands by exponential enrichment (SELEX), ...Peixuan AND Haque, Farzin. RNA Nanotechnology and Therapeutics. Boca Raton: CRC Press, 2014. 5. Hayat, Akhtar...

  16. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    ...known functions has the potential to be used in therapeutics, as it can increase gene expression at certain..., Garnett MJ (2019) Prioritization of cancer therapeutic targets using CRISPR–Cas9 screens. Nature 568...

  17. Plasmids 101: Control Plasmids

    Type
    Blog Post
    .... An internal control may be a plasmid that constitutively expresses a reporter protein (e.g., GFP or ...many variables. However daunting designing and executing these steps may be, proper controls are a basic...

  18. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ... Used since the mid-90s, this technique has revolutionised the way we apprehend molecular complexes and...wavelength, to the concentration of fluorophore in solution) are two additional parameters that can affect...
Showing: 441 - 460 of 737 results