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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 492 results for: ara-2

Showing: 441 - 460 of 492 results

  2. High Complexity Golden Gate Assembly with NEB’s DAD

    Type
    Blog Post
    ...rules are: (1) don’t use the same overhang twice; (2) avoid palindromes; (3) no overhangs with the same...highest-fidelity overhang set it can find for those parameters. For even more specificity, you can exclude specific...

  3. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ...systems utilize with type I-F Cascades. Figure 2: Cascade complex from CRISPR type I-E. Created with...been challenging. It can be difficult to ensure comparable expression of all components of Cascade for maximum...

  4. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ...that view (Callahan et al., 2022).     Fig. 2: Students in an MDH CURE replaced select residues ...Part I – guided inquiry – purification and characterization of a fusion protein: Histidine tag, malate...

  6. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    ...experience and to build upon your existing skills.  2. Find your niche Identify and develop an area of expertise...seekers to use online applications with significant character limits for the written portion of the grant application...

  8. Hot Plasmids - August 2020

    Type
    Blog Post
    ...based antiviral method to degrade RNA from SARS-CoV-2 sequences and live influenza A virus. It was demonstrated...stuck in inclusion bodies. Takayuki Iwaki, Kimiko Hara, and Kazuo Umemura have described a simple method...

  9. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ...rate of 10-55% - the PASTE system.     Fig. 2: Schematic of programmable gene insertion with PASTE...and Discovery As part of their quest to find, characterize, and develop new CRISPR systems and technologies...

  10. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ...two visible blue or magenta chromoproteins (Figure 2) (Liljeruhm et al., 2018). This system is a nice confirmation...tested their expression under both rhamnose- and arabinose-inducible promoters. Tight control of toxin expression...escape). The rhamnose induction system is well-characterized and quite tight (Giacalone et al., 2006). The...though Khetrapal et al. also demonstrated that the arabinose promoter worked too. Rhamnose induction relies...References Espah Borujeni, Amin, Anirudh S. Channarasappa, and Howard M. Salis. "Translation rate is controlled...

  11. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ... iRFP713, % Oligomeric state Photo-stabilityc, t1/2, s pKa Brightness in HeLa cells vs. iRFP713, % d ...and multicolor NIR imaging.  Small miRFPnano A separate class of NIR FPs, named miRFPnanos, were derived...developed based on mIFP6. Learn more about the characteristics, performance, and applications of near-infrared... found to be a dimer3,15. ND-not determined in parallel assays with other FPs.   We hope that this post...

  12. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ...can still record the signal separately for each target.   Figure 2: Examples of simultaneous multiplex...or formalin-fixed, paraffin-embedded tissue sections, methanol- or paraformaldehyde-fixed cell samples...samples, or other preparations. Sequential IF For a sequential IF experiment (Figure 1A), you’ll block, add...different color fluorophore that you can image separately on your microscope. Abcam's sample protocol is...different antibodies, blocking, washing, and sample preparation steps, in order to ensure your antibodies are...Ilangovan, V., Lo, H., Olson, M., Mohamed, S. Y., Sarabipour, S., Varma, A., Walavalkar, K., Wissink, E. M...

  13. Five Popular Model Organisms

    Type
    Blog Post
    ...variety of environmental conditions, and double every 2 hours. Yeast are also the first eukaryotic genome ...as Parkinson’s disease (PD). PD is primarily characterized by protein misfolding that leads to a build-...23594743. PubMed Central PMCID: PMC3703927. Jennings, Barbara H. "Drosophila–a versatile model in biology & medicine...

  14. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    ...become a valuable asset yourself.     Fig 2: A diagram of the Thomas-Kilmann Conflict Model, courtesy...favorite books, Michael Ende’s Momo, the title character endears herself to the nearby village (and to ...or how completely different teams are tackling comparable conflicts. Is this level of domain knowledge ...

  16. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    ... Watch Joanne give her "Not" Networking 101 Talk 2. Tailor every talk to the occasion Don’t just plan...come up with clear and concise answers. This preparation will help you avoid giving only yes or no answers...

  17. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    ...PubMed PMID: 3459152. PubMed Central PMCID: PMC323600. 2. Chester, Nicholas, and Daniel R. Marshak. "Dimethyl...small difference in size, the fragments should be separated on a high percentage agarose gel (~3%). Note that...

  18. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ... from damage (Abe & Lim, 2024).    Figure 2: A) When proteins (blue) interact with the air-water...RvLEAMshort (1:6 molar ratio) with 10 minutes of glutaraldehyde crosslinking: representative micrograph (C)...allowed the authors to determine structures at comparable or higher resolution than using more complex ...buffer additive MgCl2 and the crosslinking agent glutaraldehyde. Adding RvLEAMshort to the sample before plunge...

  19. AAV Purification by Iodixanol Gradient Ultracentrifugation

    Type
    Protocol
    ...Poloxamer 188 NaCl MgCl 2 KCl Centrifugal filter units (MWCO 100 kDa) Reagent Preparation 1 M NaCl/PBS-MK buffer...Add 5 mL of Buffer B and 2 mL of 5 M NaCl to 43 mL PBS Procedure Preparation and loading of the iodixanol...purification. Workflow Timeline Day 1: Purify Day 2: Buffer exchange and concentration Note: Both steps...buffer Dissolve 5.84 g of NaCl, 26.3 mg of MgCl 2 and 14.91 mg of KCl in 1× PBS in a final volume of 100...at 4 °C. 1X PBS-MK buffer Dissolve 26.3 mg of MgCl 2 , and 14.91 mg of KCl in 1× PBS in a final volume ...need more time, you can alternatively centrifuge for 2 h at 200,000 x g at 18 °C. Carefully take the QuickSeal...interface of the 60% and 40% gradient (see Figure 2) with an 18 ga needle. Place the first microcentrifuge...

  20. AAV ddPCR Titration

    Type
    Protocol
    ... 95 10 2 1 Denaturation 95 0.5 2 50 Annealing/Extension 60 1 2 50 Signal Stabilization 98 10 2 1 Hold ...should decrease by a factor of 2 across the dilutions. In the example below, 2-fold serial dilutions of a ... considered biosafety level 1 but may require BSL-2 handling depending on the insert. Please ensure that...single channel pipette 1–10 µL multichannel pipette 2–50 µL multichannel pipette 20–200 µL multichannel ...20X): 5 µL in 95 µL 1X PCR buffer (1:20) Dilution 2 (20X): 5 µL in 95 µL 1X PCR buffer (1:400) Dilution... DG8 cartridge into the cartridge holder. Using a 2–50 µL multichannel pipet, load 20 µL of the reaction...Hold 4 ∞ 2 1 After PCR is complete, transfer the plate to the Droplet Reader. Open the QuantaSoft software...
Showing: 441 - 460 of 492 results