We narrowed to 599 results for: arl

1. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

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   ...and quantitative analysis of epigenetic memory at nearly any locus. The FIRE-Cas9 plasmids can be found ...

2. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

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   ...than tracking generic autophagy markers. It’s particularly useful for comparing bulk autophagy vs. mitophagy...

3. Human Germline Editing Using CRISPR

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   ... appealing for heterozygous mutations, it will clearly be much more challenging for homozygous mutations...

4. Lighting Up Cell Signaling with Photoswitchable Kinases

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   ...versions of MEK1, MEK2, Raf1, and CDK5 all behave similarly to their endogenous counterparts. Additionally...

5. Seven Tips for Using LinkedIn as a Scientist

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   ...to send invitations. Finally, if you don’t know clearly why you are inviting someone to connect, perhaps...

6. Troubleshooting Your Plasmid Cloning Experiment

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   ...having difficulties in amplifying a template, particularly from a large genome, you can recheck your primer...

7. Giving gRNAs a Facelift - Synthetic and Beyond

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   ...et al., Frontiers in Genome Editing). This is particularly important in experiments in which viability ...

8. Antibodies 101: Conjugation

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   ...touched on fluorophores as antibody conjugates in an earlier post, where you can find some additional tips for...

9. What's New in CRISPR - November 2021

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   ...Building upon the PE2 and PE3 systems of their earlier work, they developed a system in which the cellular...

10. Antibodies 101: Polyclonal Antibodies

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    ...increased sensitivity, polyclonal antibodies are particularly useful for certain research applications. Many...

11. Developing Lab Management Software for Biology

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    ...things will trip everyone up at some point, decide early on what to do in a namespace collision. It may even...

12. Gendered Innovations: Why Does Sex of the Cell Matter?

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    ... important point, however, is that cost at this early stage of research is minimal compared to that of...

13. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

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    ...Sciences of the University of Hong Kong. He is particularly interested in developing and applying new technologies...

14. Addgene's Tips for Plasmid Quality Control

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    ...have a few tricks to simplify the process. First, nearly all of our Sanger sequencing is done from glycerol...

15. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

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    ...SpCas9-HF1 Kleinstiver et al and the Joung Lab similarly thought that if they could weaken sequence independent...

16. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

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    ...and CFP can activate certain T-DDOG constructs similarly to GFP, but commonly used red fluorescent proteins...

17. New Tool for Lineage Tracing: The ClonTracer Library

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    ...Vivo Pharmacology at Novartis Oncology and is particularly interested in applying what we have learned ...

18. 5 Steps to Writing Grant Proposals for Grad Students

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    ...that the goal and the impact of the project are clearly stated and align with the description of the RFA...

19. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

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    ...biology and genetics at Boston College. She is particularly interested in strategies to incorporate research...

20. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

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    ... to interact by stimulation with blue light.  Similarly, light can also be used to control homo-oligomerization...