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Showing: 441 - 460 of 976 results

  1. Tips for Technical Support Calls

    Type
    Blog Post
    ... are your best bet to figure out how to use a particular reagent.  We also have links to later papers ...look! This stuff works!” These papers can be a particularly good resource if you are considering using a...serious science questions, consider using Google Scholar, which searches scientific articles, patents, and.... Addgene keeps a Document of Gratitude from particularly enthusiastic customers who have enjoyed their...combatitive. Not every question is answerable, particularly when it comes to technical questions. BUT, I...

  2. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

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    Blog Post
    ...CRISPR variety (see slides from my talk below). Largely organized by Dmitry Samarsky, and a very welcoming...Szostak’s lab continues its work on the origin of cellular life. His lab has long hypothesized that life ...Nikolaus Rajewsky from the Max Delbruck Center for Molecular Medicine presented another unexpected finding ...regulatory RNA field. The Rajewsky lab is studying circular RNAs (who knew…I had barely heard of these). These...abundant in neuronal synapses. shRNA silencing of one circular RNA they have identified resulted in a cell migration...

  3. Gaining Leadership Skills Volunteering at a Professional Organization

    Type
    Blog Post
    ...Management skills”. The NPA states that Postdoctoral scholars should have the skills and techniques needed to... how to adapt to different situations and, in particular, to different styles of leadership. As the Chair...Committee would change format depending on the particular style and requirements of the new Board Members... more independently and some others would need regular re-direction and alignment with the overall mission...organization to develop these leadership skills in particular because every year it organized a “Committee ...

  4. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    ...after receiving her degree she was faced with a similar conundrum – she had the skills, training, and motivation...our ideas got pretty good. We started doing extracurricular research on some of them to see how feasible...in my lab notebook from time to time. I wrote in large letters “Going for it!” We kept our day jobs and...company, and that’s what we want it to do. It’s exhilarating to have that sort of freedom. It can also be...and the ocean. After receiving her bachelors in Molecular Biophysics and Biochemistry from the Illinois ...

  5. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    ..., there may be particular poster you are interested in viewing which has drawn a large crowd, making it...don’t fully understand the question, ask for clarification. Having them reframe or elaborate their thought...posters you are interested in viewing. For most larger conferences, poster titles and abstracts are available...permitting, you can go back to the most interesting and popular posters at the end of the session. Get in contact...

  6. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    ...the conference you are attending on the larger side? At large conferences, chances are that several talks... the other person seemed confused, bored, or particularly excited about? If you tried tweaking your pitch... contact information if your conversation is particularly fruitful. After the conference Bring back what...LinkedIn or Twitter, for instance. If you’ve had a particularly meaningful conversation, and you have their ...

  7. Pooled Library Amplifications

    Type
    Blog Post
    ... prone to recombination, while a library with a large backbone might be more difficult to transform.  ... per transformation may vary depending on the particular type of cells you are using, as well as other...generally needs to be determined experimentally. For larger libraries that require hundreds of nanograms of...full-size plasmids (or for smaller plasmids to overtake larger plasmids, if the library varies significantly in...looking at protocols used for libraries that are similar in size and composition as examples. Table 1 provides...

  8. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...pathways and represent the largest family of human drug targets. To enable large-scale profiling of many ...then used single-molecule FRET to study the intramolecular dynamics of the proteins in solution and when...by endogenous GPCR. Example applications include large-scale pharmacological profiling or interrogation...conditions, revealing context-dependent responses and molecular patterns of selectivity. With 10 plasmids available...

  9. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

    Type
    Blog Post
    ...also possess similar morphologies, growth properties, and express stem cell markers similar to ESCs [11...lentiviral infections respectively [9, 10]. These stellar works led to a Nobel Prize for Dr. Yamanaka in ...11].  While iPSCs and ESCs have similar functional utility, iPSCs bypass the transplantation compatibility...2. Jaenisch, R. and R. Young, Stem cells, the molecular circuitry of pluripotency and nuclear reprogramming...

  10. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    ...Medicine. She is particularly interested in science communication and fundamental molecular biology.      ... it. About three or four years into my PhD in Molecular Biology, I realized I wanted a career in science...experience taught me. The article submitted was hilarious, full of interesting information, and received...researchers. It is an excellent way to meet people with similar interests! Especially as someone interested in ...

  11. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ...the same cassette is sometimes advantageous, particularly when only a portion of the plasmid is packaged...discovered - many from viruses, but also some from cellular mRNAs. What they all have in common is the ability...have some disadvantages. These elements are quite large (500-600 bp) and may take up precious space in viral... are short (about 20 amino acids) and produce equimolar levels of mulitple genes from the same mRNA. The...posts or browse Addgene's Plasmid Guide for more molecular biology and cloning information. ...

  12. Deep Dive: qPCR

    Type
    Blog Post
    ...actions to a single step. Scorpion chemistry in particular has been reported to work well in the Lightcycler...specifically aimed at determining the best options for a particular cell or tissue type (such as here, here, or here...be worth the initial effort of setting it up for larger or frequently analyzed sample types and sets.   ...analogous Wilcoxon tests have all been shown to give similar results, so you only need to pick your favorite...Real-Time PCR: Methods and Protocols, Methods in Molecular Biology, vol. 1160, DOI 10.1007/978-1-4939-0733... S; Huggett J. qPCR primer design revisited. Biomolecular Detection and Quantification. Volume 14. 2017...

  13. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

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    Blog Post
    ...tools for efficient and programmable insertions of large DNA cargos into bacterial genomes. This new capability...Type II systems (such as CRISPR-Cas9). Unlike in regular Type I CRISPR-Cas systems, however, Cascade from...V-K CRISPR-transposon systems function in a very similar manner to Type I-F, but instead employ a Cas12k...CRISPR-transposons excel at programmable insertions of even very large genetic cargo, at one or multiple desired genomic...systems have already been demonstrated, ranging from large genomic insertions and/or deletions, to multiplexed...

  14. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    ...and injection methods are best suited to their particular scientific question - even when others have already...to successfully replicate published works. Of particular importance to these efforts is the open sharing...Reports are reviewed by Addgene scientists for clarity and accuracy, and by the end of the year, those...Chemically and Functionally Distinct Vectors. Molecular Therapy - Methods and Clinical Development, 18...occurrence during vector purification and formulation. Molecular Therapy, 12(1), 171–178. https://doi.org/10.1016...

  15. Plasmids 101: Choosing an Antibiotic Resistance Gene

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    Blog Post
    ...other antibiotics listed below affect continuous cellular processes (e.g., protein synthesis), while amp...synthesis as their mode of action and have a very similar relationship as amp and carb (they are interchangeable...all bacteria used in research, it isn’t a widely popular selection agent. Pros: Stable (spectinomycin). .../1475-2859-11-56 Trastoy, M. O., Defais, M., & Larminat, F. (2005). Resistance to the antibiotic Zeocin...gei016I Resources on Addgene.org Addgene’s Basic Molecular Biology Protocols Over-Agar Antibiotic Plating...

  16. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type
    Blog Post
    ...enhancer it targets is a “pillar of function” for our stem cells. Remove this pillar and the ESC dies. Order...knowledge of which stretches of the genome are pillars for stem cells, or metastasized tumor cells, or... a double helix. People are wrong. In reality, cellular DNA is a complex three-dimensional globule of ... 10 meters apart, are mere millimeters apart. Similarly, cells compact DNA in a way that brings linearly...

  17. Cpf1: A New Tool for CRISPR Genome Editing

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    Blog Post
    ...GC-content. After sequencing and searching for cellular RNAs important for Cpf1 function, they found that...found two that display robust cleavage activity similar to that of Cas9. These two nucleases, AsCpf1 and...respectively), both cleave in a staggered pattern similar to FnCpf1. Potential advantages of Cpf1 over Cas9.... Sticky-end mediated gene transfer would be particularly helpful for targeting non-dividing cells, which...et al. suggest that AsCpf1’s off-target rate is similar to that of high fidelity Cas9s eSpCas9 and SpCas9...

  18. CRISPR 101: Cytosine and Adenine Base Editors

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    Blog Post
    ... and DNA repair. Black arrows indicate natural cellular processes. BER = base excision repair. Note: hypoxanthine...nickase (Nishida et al., 2016). Target-AID acts similarly but not identically to BE3, modifying a 3-5 base...adenine deaminase TadA.   After seven rounds of molecular evolution, they obtained four adenine base editors...Cas9 (Grünewald et al., 2020). Another lab took a similar approach. Dali Li’s lab’s A&C-BEmax consists of...10.1038/s41587-020-0491-6 Grünewald, J., Zhou, R., Lareau, C. A., Garcia, S. P., Iyer, S., Miller, B. R.,...T. P., Raguram, A., Banskota, S., Newby, G. A., Tolar, J., Osborn, M. J., & Liu, D. R. (2023). Evolution...

  19. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    ...restriction enzyme cloning. Instead, you can choose a molecular cloning technique that will work well with a given...1990s, Gateway cloning technology has become very popular as a rapid and highly efficient way to move DNA...has ready-made entry clones available for many popular genes, including Hs.KRAS4a. Use our website to ...Addgene’s ready-made entry clones can be used with a large variety of plasmids. Speed The Gateway system enables...Diagnostic Restriction Digest Video Check Out Our Molecular Biology Reference Pages  ...

  20. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    ...complex approaches to scientific questions become popular. There are only so many methods and machines one...established partnership. If you’re willing to do similar things for your team or lab, you’re likely to be... talking to them at a seminars, or even set up regular meetings. Meeting people outside your organization...I find it useful to remind myself to check in regularly with collaborators for example on a holiday or...regard to reaching out to new people, checking in regularly, and maintaining useful notes that remind me about...
Showing: 441 - 460 of 976 results