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...libraries, large collections of biological independent variables, are becoming increasingly popular tools ...Viruses are the SUVs of molecular biology – they can deliver materials to specific destinations, haul...explored as the delivery mechanism for increasingly popular nucleic acid-based vaccines. These vectors provide...advantages over traditional vaccines, including 1) cellular responses in addition to antibody response 2) ...

...colleague who preferred detailed instructions and regular check-ins, which helped them stay on track and ...resource: connecting with mentors who have made similar transitions can provide you with insights, lessons...mentoring a junior colleague. Guiding an intern, in particular, served as a great "baby project," offering a...strengths. By encouraging alignment on goals and clarifying roles, I eased the tension and improved collaboration... his PhD and a couple of postdocs focusing on molecular biology and metabolism. He now guides fellow PhDs...

...interchangeable. General envelope plasmids, such as VSV-G (Vesicular Stomatitis Virus glycoprotein), however, may be...Packaging using 293T cells This method is very similar to lentiviral packaging methods. The three components...constructs. Why do many γ-retroviral plasmids have similar names? The nomenclature of the plasmid backbone...Type Element Purpose Envelope VSV-G Vesicular stomatitis virus G glycoprotein; Broad tropism...Viral Vector Elements The Retroviral Life Cycle Popular Retroviral Vectors and Their Use in Research More...

...is an app that helps you find internships and scholarships in different subject areas!    2. Resume...size, legibility, and more). Tips on drafting a stellar resume and cover letter can be found on the Addgene...through. The vast majority of programs tend to ask similar questions, thus devoting some time to writing a... for them, the likelier you will end up with a stellar letter of support.        5. Application submission...number of years as you apply for internships, scholarships, fellowships, and entry-level positions post...

... You can also find a list of popular sequencing primers in our Molecular Biology Reference. Sometimes ...sequencing or the other techniques mentioned above. In molecular biology, cloning a gene into your plasmid is often...plasmid basics in our Plasmids 101 series Find more molecular biology protocols and tips Resources on Addgene.org... Learn more about Sanger sequencing Read the Molecular Biology Reference ...

... Information Resources. ILAR Journal 46:258–268. https://doi.org/10.1093/ilar.46.3.258  Mould AP, Askari...for your experiment Monoclonal antibodies are particularly useful when you want to ensure that the antibody... the antibody, meaning that the antibodies are largely identical between lots.  Disadvantages of monoclonal..., Wong J, Koh XY, Eng H-Y, Lane D, Lefranc M-P, Clark M, Dübel S (2018) When monoclonal antibodies are...

...to studying membranes, organelles, and other subcellular compartments is via proteomics--the study of ... norovirus. While this assay is specific to a particular strain of norovirus, it can be tailored for the...segment The human proteome contains a breadth of modular domains that facilitate protein-protein interactions... and mediate biochemical processes. One of the largest and best characterized of these is the Src homology... sequencing, revealing that they bind to a much larger subset of protein motifs than previously thought...

...themselves using computational approaches to analyze large data sets (myself included) and we are faced with... lost in the wide word of informatics. I am a molecular biologist who began coding for my thesis project...will keep you from repeating your work or making similar mistakes in the future. Stay organized Set up...for those that are just starting out, and helps clarify the role of the code. # This is a comment in python...student at University of Pennsylvania. She is particularly interested in science communication and epigenetics...

... will provide you with an overview of general molecular biology knowledge and techniques. If you are interested...our screening overview blog post.) *Note: Two Popular Vectors for Blue White Screening Available at Addgene...of this gene, disrupting the lethality. This is similar to α-peptide DNA disruption in the blue-white screen...may contain genes that allow for the use of a particular substrate in defined medium, without which the...Resources on the Addgene Website Choose the Best Molecular Cloning Technique for You Browse Addgene's curated...

...basics of molecular biology and cloning, since they have reasonable introductions to lots of molecular biology...quick flash back to 2004. “Shrek 2” was the most popular movie in theaters, the Razr was the hottest phone...biology,” — Reddit commentor Yay4sean, in r/molecularbiology.   Oh, to be young again! Addgenie Marcy...ready-to-use viral vectors from some of our most popular lentiviral and AAV vectors, as well as developing...

...the past several years, it seems that the more polarized (negative) the action potential of a cell, the...differentiated and quiescent it is, and the more depolarized (positive) the cell is, the more embryonic, stem...cation channel) light-activated channels to hyperpolarize tumor cells, which resulted in tumor regression...activation, it stays open for a longer duration. Hyperpolarizing chloride channel SwiChr++ - A channelrhodopsin...and gap junction protein-mediated cues control polarity during planarian regeneration." Developmental ...

...organizations with similar ideas who were also thinking about reproducibility and shared similar desires to take...talented and motivated groups and individuals with similar ideas to actually do something about it. Building...workshop. These workshops illustrate the open and modular nature of the workshop content: different parts...results, and tracking material downloads. This was particularly helpful for securing funding to support this...

...academic lab, a similar situation could arise if a lab veteran who worked with a particular sample suddenly... lab management software aren’t just limited to large volume facilities; it can be useful in academic ...you sad),  someone has put in a request for a particular plasmid and now it’s time for our lab technicians...this, it is important to write loosely coupled, modular code. Use tried-and-true tools We use Python, Django...

...these mutants are not toxic to HEK cells and that similar mutations can improve the specificity of Cas9 derived...from S. aureus. This means you can likely apply similar rational mutations to your Cas9 homolog of choice...SpCas9-HF1 Kleinstiver et al and the Joung Lab similarly thought that if they could weaken sequence independent...found in eSpCas9, the effect on specificity was similar; SpCas9-HF1 generated fewer off-target cuts when...variant displays comparable on-target activity and similar or decreased off-target activity across the sites...

...as T-DDOG and Cre-DOG, respectively, repurpose popular GFP reporter lines for more sophisticated experimental...and CFP can activate certain T-DDOG constructs similarly to GFP, but commonly used red fluorescent proteins...activating gene expression. Although Cre isn’t a modular protein, Tang et. al were able to create a split...to many types of proteins. For proteins with a modular structure, constructing split variants should be... also possible to create split versions of non-modular proteins like Cre. Could Cas9-DOG be developed ...

...perform more modern techniques used in genetics or molecular biology because the experiments are either not...templates which they can easily generate off of a circular plasmid via PCR. Typically, to make these constructs...complementary DNA (cDNA), and then use PCR and molecular cloning to insert the cDNA into the desired plasmid...those who are interested in performing their own molecular cloning and investigating genes not in this collection...vector backbone. The empty vector PLL4, which is similar to PR-T4P, is available from Addgene. If you use...

...the bait at a particular point in time, i.e. when the bait is interacting with a particular protein partner...specifically identify proteins that interact with a particular protein  complex as opposed to an individual ...partner or when the bait is part of a particular protein complex. Not performed in a native cell context...bait proteins stop interacting. This feature is particularly useful for in-depth characterization of dynamic...

...reproducibility of experiments. Inbred mice are particularly useful for understanding a specific effect related...that alters or eliminates the expression of a particular gene. Targeting vectors used to create knockout... a way to track successfully engineered cells. Similar to transgenic mice, knockout mice are engineered...off-target effects on regions of the genome with similar homology to the site of interest or create mosaic...recombination of floxed sites in the cell can occur. Similar systems include tetracycline or doxycycline mediated...

... the digital music revolution hit. I predict a similar revolution for antibodies. Recombinant antibody...that may not work well in animals because of similarities to host proteins. After the antibody of interest...long-term stability, consistency between batches, and molecular definition of recombinant antibodies are essential...US alone (Bradbury & Plückthun, 2015a). With molecularly defined antibodies that do not change over time..., Wong J, Koh XY, Eng H-Y, Lane D, Lefranc M-P, Clark M, Dübel S (2018) When monoclonal antibodies are...

...friendly, to own the process entirely remains a large undertaking. Priority number one was to ensure that... There are regions of some plasmids that are particularly difficult to sequence and assemble, including...regions that are GC rich. Since NGS assembles a larger sequence from many smaller sequences, regions with...some plasmids will not result in one complete, circular assembly. Most of the time, these plasmids are...Viral preps and pooled libraries In addition to regular plasmid sequencing, many of you will be familiar...