We narrowed to 810 results for: mal
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TypeBlog Post...5], Sendai virus [6], plasmid electroporation (episomal) [7, 8] and mRNA transfection [9]. Many of the...differentiation.” Specific cell media, supplements, bioactive small molecules, and growth factors are used to control... While natural transdifferentiation is rare in mammals, an example is observed in the pancreas when excess...
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How to Deposit Your Plasmids with Addgene
TypeBlog Post...lentiviral vector to be used in mammalian cells, check Lentiviral and Mammalian Expression). This information...differing in the gene/insert. Pooled libraries are normally supplied as liquid DNA with all of the plasmids... -
The 12 Days of CRISPR: 2021
TypeBlog Post...gave to me: a miniature CRISPR-Cas system for mammalian use. On the second day of CRISPR, Addgene gave...Addgene gave to me: MUCICAT to perform multicopy chromosomal integration. On the tenth day of CRISPR, Addgene... -
8 Great Depositor Research Stories
TypeBlog Post...editing and multiplex prime editing (requiring minimal payloads!) Uncovering Ancestral Bias in CRISPR ...Miles on everything from AAV prep to targeting to animal models. It was one of our favorite posts from 2022... -
Top Requested Plasmid of 2017 - pMD2.G
TypeBlog Post...which are used to deliver Cas9 and other genes to mammalian cells. For this reason, the top requested plasmid...envelope protein under the control of the powerful mammalian CMV promoter. Envelope proteins are a key component... -
Addgene Protocols Go Viral: AAV Purification and Titration
TypeBlog Post...started with tissue culture The cultivation of mammalian cells in the lab, or tissue culture as it is commonly...called, is a critical tool for many scientists. Mammalian cells provide scientists with the means to study... -
Interview with Bart Newland, Addgene’s Newest Board Member
TypeBlog Post...the transition now to being general counsel of a small start-up. For the last 20 years, I was a patent ...very different jobs. Being general counsel at a small start-up is very interesting. It’s very varied. ... -
Inntags: Innovative Protein Epitope Tagging
TypeBlog Post...References 1. Georgieva, Maya V., et al. "Inntags: small self-structured epitopes for innocuous protein tagging... high correlation for protein localization in mammalian cells." Nature methods 10.4 (2013): 315-323. Pubmed... -
Editor's Choice, September 2016
TypeBlog Post...progressiva (a.k.a. FOP), that, while affecting a small number of people, deserves our attention and has...Plasmid Formats Better Dyeing Through Chemistry & Small Molecule Fluorophores Addgene's Cancer Collection... -
Management for Scientists: Seeking Feedback
TypeBlog Post...opinions in meetings, via email, at formal communications events or at informal gatherings, like at lunch. Many... -
Six Spooky Science Stories and Halloween at Addgene
TypeBlog Post...there until they mature and changes sex from male to female. Then, it detaches from the gills and latches... -
Seeing Red: Simple GFP Photoconversion
TypeBlog Post...has been shown to work in plant, Drosophila and mammalian cells, and it may find wide use in biological ...efficiently photoconverted in plant, Drosophila, and mammalian cells. Photoconverted GFP is compatible with other... -
Science as Art
TypeBlog Post... is has been featured in Time magazine . Other smaller competitions are now taking place and gathering...moonlighting as artists can compete in the Nikon Small World Photomicrography Competition - a 40 year old... -
Tag Your Favorite Yeast Genes with Ease
TypeBlog Post... 50 base pairs of sequence homologous to the chromosomal site of insertion. The linear PCR product is ...Based on their findings, the authors recommend optimal FP combinations for use in yeast imaging, categorized... -
CRISPR 101: Drug discovery and CRISPR
TypeBlog Post.... – CRISPR can be used to generate cell line or animal models which have the precise disease genetics.... drug selectively kills the wildtype line with minimal to no toxicity in the knockout line, it is likely... -
Plasmids 101: Gene, enhancer, and promoter trapping
TypeBlog Post... reporter gene flanked by a minimal promoter which can integrate proximal to an endogenous gene to turn... -
Chemogenetics vs. Optogenetics: Which Method Should I Choose?
TypeBlog Post...the same in both cases, but activation depends on small molecule activation (chemogenetics) or light (optogenetics.... TL;DR If precise spatiotemporal control of a small subset of neurons is necessary, use optogenetics... -
Fostering communication in remote collaborations
TypeBlog Post...and improves overall communication. Maintain informal communication In between recurring meetings, you...this casual communication reflects the type of informal communication you’d likely have with members of... -
Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics
TypeBlog Post...downloadable in a small file size with a Biorender watermark, which is usually ok for informal settings like... -
Cellosaurus: A Cell Line Information Database
TypeBlog Post... of interest. You can filter CLASTR results by minimal number of markers and similarity score match. This...allele search preferences to help you identify proximal lines you care about. If you have cell lines without...