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We narrowed to 850 results for: met

Showing: 441 - 460 of 850 results

  1. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type
    Blog Post
    ...exported as a picture. I believe that showing something different from time to time breaks up the monologue...monologue a little and gives participants something else to focus on. Use collaborative cloud apps It is... Take brain breaks Meetings can get very long sometimes, and interaction fades away the longer we are ...

  2. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    ... et al 2016) revealed some of the biophysical parameters that can influence the HDR/NHEJ decision. Our...or double stranded donor DNA with other design parameters. Example single stranded donor DNA and a presumptive...catalytically active and inactive CRISPR-Cas9 using asymmetric donor DNA." Nature biotechnology (2016). PubMed...

  3. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    ...probe and build new systems. Such goals require methods to directly activate and inactivate biological ...that prints a chemical image, as well as a genetic method for the computational problem of edge detection...identified in bacteria, and with similar optimization methods, many of these systems could be made suitable for...

  4. Why and how to start studying chromatin (with Addgene's help)

    Type
    Blog Post
    ...gene a megabase away, or looking at the histone methylation status at a double-strand break that needs repairing...guest bloggers have written posts describing new methods and tools for studying chromatin and the epigenome... and Editing the Epigenome CUT&RUN: An Improved Method for Studying Protein-DNA Interactions CRISPRainbow...

  5. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

    Type
    Blog Post
    ...characterized orthologs from “Anaerobic digester metagenome” (AdmCas13d) and Ruminococcus flavefaciens (nicknamed...87% mCherry protein knockdown measured by flow cytometry, respectively. Cas13d CRISPR array processing ... al. compared CasRx to multiple RNA regulating methods: small hairpin RNA interference, dCas9-mediated...

  6. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    ... a look at a few screening methods. Blue-white screen A widely used method of screening is the blue-white...sequencing primers in our Molecular Biology Reference. Sometimes common  primers can't provide all of the information...

  7. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    ...variety of cloning techniques and troubleshooting methodologies drew me to learn more about the process that...immunofluorescence-immunocytochemistry (IF-ICC.) This method was chosen because cells transfected with a plasmid...available from each plasmid webpage. Importantly, the methods developed by the Trimmer Lab for construction and...

  8. Hot Plasmids - February 2022

    Type
    Blog Post
    ...Clouse While many CRISPR-based genetic screening methods have primarily used Cas9 to knock out genes, researchers... screening toolkit by optimizing a Cas12a-based method for screens. Cas12a’s ability to perform multiplex...in parallel. Lastly, this Cas12a-based screening method was used to create a genome-wide library capable...

  9. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    ... which non-canonical amino acids (ncAAs, also sometimes called unnatural amino acids) are placed in a .... And, naturally, rarely are the optimization parameters the same for all three components, meaning that...Griffith, M. C., & Schultz, P. G. (1989). A General Method for Site-specific Incorporation of Unnatural Amino...

  10. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    ...understand how the brain works. There are two methods of neuronal tracing (Fig. 1): one to visualize ...catecholamine pathways from the spinal cord (Wang, 2018, Metcalfe, 2022) and substantia nigra (Tervo, 2016). Discrepancies...neurons retrogradely transduced by the vector. One method for minimizing the impact of this phenomenon is...

  12. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

    Type
    Blog Post
    ...transgenic flies to study your favorite gene (YFG).  Sometimes, you want to use the Gal4/UAS system, but the ... depending on your interests. You can look at something as basic as survival. For those interested in ...immunofluorescent images like in this paper (2). Metabolic assays such as Seahorse can be employed to look...

  13. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type
    Blog Post
    ...both the biological system as well as the imaging method, we recommend basing your selection on a small-...is calculated by multiplying EC by QY (EC*QY, sometimes normalized to the value of EGFP). The higher the...practical brightness takes all application specific parameters into account, including the specs of your microscope...

  15. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

    Type
    Blog Post
    ...transcription factors, starting with an established method called ChIP-seq. ChIP-seq relies on antibodies ... hundreds of transcription factors. We wanted something that would allow automation. This design cuts ...pressure on many other labs that may be doing something similar. There may be other variants of this that...

  16. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    ...subgroup of researchers in your area of expertise? Sometimes, the funders of your research may stipulate that...models, and software tools in the Materials and methods section of their manuscript to make resources easier...information you provide on data, materials, and methods, the more valuable your paper will be to the scientific...

  17. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    ...to understand the basis for cardiovascular and metabolic human diseases. They do that by studying patients...number of genes involved in cardiovascular and metabolic diseases. We are knocking out genes that have ...to examine the effect of a gene on cholesterol metabolism. The TALEN kit is quite useful in generating ...

  18. Antibodies 101: Fab Fragments

    Type
    Blog Post
    ...super-resolution microscopy. Cell Rep Methods 2(10):100301. https://doi.org/10.1016/j.crmeth.2022.100301  Additional...affinity reagents, and for good reason. However, sometimes you want a tool that is a little more compact ...

  20. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    ...diversity and flexibility of diabodies. Methods. 10.1016/j.ymeth.2018.09.005 Muyldermans S. (2013). Nanobodies...including proteins, peptides, amino acids, drugs, metal ions, and cells. They can be used either with or...
Showing: 441 - 460 of 850 results