We narrowed to 775 results for: mos

...during tissue collection and homogenization. The most widely known of these products is TRIzol® (also ...researchers can do to combat this issue. First and foremost, be mindful of your extractions- if you need really...

...The shredder What sets Cas3 apart from Cas9 and almost all other Cas enzymes is its method of DNA cleavage...abilities   Choosing a Cas While Cas9 may be the most adaptable Cas currently available, the other Cas...

...sharing your science - which can drive away even the most enthusiastic of learners.  What contributes to cognitive...audience to take on, just to understand you at the most basic of levels.  Concepts This can be obvious, ...

... a number of research and clinical applications. Most of the time, when someone speaks about an affinity...thing to go when downsizing an affinity reagent, most of the alternative reagents have reduced immunogenicity...

...was successfully used in conditions that are the most similar to yours. Take a look at the publications... You now know that application validation is the most important factor in antibody selection, but what...

... is further processed to isolate the antibodies. Most commonly, antibodies of the IgG isotype are used...When to stay away from polyclonal antibodies The most prevalent issue with polyclonal antibodies is their...

...it is unpredictable which fluorescent protein is most photostable for each of these conditions. On top...power used for live-cell imaging. To conclude, the most photostable fluorescent protein can be identified...

...conjugate class than fluorophores are enzymes. The most common enzyme conjugates are Horseradish Peroxidase...be achieved through several different mechanisms. Most take advantage of chemical groups intrinsically ...

...dry lab scientists (or vice versa), then you have almost certainly experienced some of the hurdles I needed...needed to overcome.  I eventually pinpointed and (mostly) solved two main problems with Addgene’s cross ...

...relationships (also known as “networking”), one of the most common questions I receive is “Do I have to be on...to you (unless they’ve chosen to hide it). While most people use LinkedIn messaging, not everyone likes...

...isolate proteins, this blog will focus on one of the most common, purification based on the polyhistidine ...containing two or more adjacent histidines are present in most production systems and can bind and co-elute with...

... 145,000 more quality-adjusted life years. While most of the results were positive, the analysis did find...et al. provide a table indicating the sex of the most commonly used cell lines appearing in American Journal...

...big promise is orthogonality. You can create an almost limitless number of DNA binding proteins with Cas9...genetic parts for complexity. We have characterized almost 600 terminators. That gives us enough hardware ...

...://pubmed.ncbi.nlm.nih.gov/32675376/ mGold, the most photostable yellow fluorescent protein to date By... as bright to mVenus, the parent YFP, and is the most photostable YFP to date. As such, mGold is expected...

...traditionally introduced in Caenorhabditis elegans on extrachromosomal arrays can lead to artifacts due to overexpression...flow cytometry. Cells grown in high IL-3 showed mostly blue fluorescence, indicating a quick cell cycle...

..., Konermann et al., 2015, Chavez et al., 2015). Most popular methods for CRISPR activation CRISPR activation...activation While SAM, SunTag, and VPR have become the most popular methods for CRISPR activation, research ...

...While Streptococcus pyogenes Cas9 (SpyCas9) is the most used and well studied CRISPR system, N. meningitidis...work? Anti-CRISPRs proteins are highly diverse but most block CRISPR activity one of three ways: Inhibit...

... because I truly enjoy the field I am in, the atmosphere, and the people.” My absolute favorite response...Operations Specialist. She said, “I’ve found the most purpose at Addgene being myself. I like being with...

... the targeting scope of adenine base editors The most commonly used S. pyogenes Cas9 requires the target...ABE7.10 with ABE8.20-m reducing protein expression the most in primary human T cells. While editing single genes...

... strands in the canonical beta-barrel structure. Most frequently, the proteins are split between strand...Hu Green sfGFP Split super-folder GFP, our most-requested GFP(1–10) and GFP(11) Versatile protein...