Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 938 results for: mpl

Showing: 441 - 460 of 938 results

  1. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

    Type
    Blog Post
    ...coordination to more complex situations such as learning and memory or emotional states, for example anxiety or ...and cause variability in the testing group. For example, if an experimenter picks up a mouse from the home...kHz, produced from fluorescent light bulbs, for example) that are outside the humans’ audible spectrum.... have preferences for specific cell types. For example, AAV1 and AAV9 serotypes have strong neuronal tropism...

  2. How to Prepare for an Industry Interview

    Type
    Blog Post
    ...task, action, and results. While this method seems simple, I found it hard to remember to describe all four.... This is a chance to highlight your prior accomplishments and demonstrate your science communication ...interview. Expect to be tired once the interview is complete Industry interviews are long! Mine lasted 4.5 ...interviewer and then thank them once the interview is complete. Remember, your interviewers took time out of ...a question. Maybe you ask for questions after completing each section of your talk. Some video conferencing...

  3. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    ... the mutation or replacing the gene. A notable example is the treatment of Severe Combined Immune Deficiency...normally functioning T cell repertoire (1). In this example, it was possible to transduce the T-cells outside...based delivery techniques may hold the key. In the simplest form, translating gene therapies from conceptual...already have ways to prevent AAV infection. For example, prevalence of neutralizing antibodies against ...: 14566358.  2. Hirsch, M. L., et al. "Self-complementary AAV mediates gene targeting and enhances endonuclease...

  4. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    ... don't do anything. Viruses are complicated and they perform complex biological tasks, both of which ...virus-based experiment at one point or another. After amplifying a viral vector, collecting virus, and transducing...replication. To avoid genomic rearrangements, try amplifying the viral vector with bacteria designed to minimize...vector using the harvested viral solution as a PCR template. Regardless of the type of virus you’re working...

  5. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    ...and look at them under a microscope, fractionate samples to isolate particular organelles and their contents...fluorescent proteins have usurped old methods or complemented them in ways that make them much easier. A special...localized to a specific region of the cell. For example, if the ratio of red/green fluorescence falls within...protein or protein fusion. This one protein method simplifies cloning and makes your experimental setup less...in gene expression. In a developing embryo, for example, a researcher could determine whether or not newly...

  6. PhD Applications After COVID

    Type
    Blog Post
    ...know that this process only became even more complicated with COVID-19 and standards for hybrid, virtual... tends to go something like this:   However, as simply as it seems laid out above, there are many places...where the PhD application cycle becomes much more complex. “How do I reach out to potential mentors?” “Where...very valid questions, and often make an already complicated process even more difficult. I’ve learned that...potential programs and universities stressful or simply not feasible. Through the virtual systems created...

  7. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    ...when studying them since their complete knockdown is lethal. Incomplete or titratable knockdown, however...the tools scientists can use to study them. For example, gene knockdown with CRISPR interference (CRISPRi...pathogenic strains and environmental isolates. For example, Peters and colleagues used Mobile-CRISPRi to knockdown...if that gene is a target of an antibiotic. For example, let’s take a look at the essential gene folA which...

  8. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ...many aaRSs exist within a multi-tRNA synthetase complex, the team investigated whether the generated antibodies...antibodies were able to immunoprecipitate the entire complex in three different cell lines. The Gräslund lab...jbc.RA120.01289     Novel protein cage provides simple enzyme protection in yeast bioproduction pathways... a reaction pathway, the MPyV system provides a simple, efficient method for protecting a single enzyme...SaCas9. A new yeast inducible CRISPRi library - Ampl133 targets S. cerevisiae genes with 6-12 guides per...

  9. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    ...remove endonucleases (see figure at right for an example). Look out for carbohydrates Certain strains, including...the cell lysis process. To ensure that lysis is complete, use a reagent like Qiagen’s LyseBlue, which uses... uses color to show you if lysis is incomplete. Protein expression strains are not good for cloning Bacterial...yields. Why would volume make a difference? In the simplest of terms, more volume = more cells = more plasmid...the material used for inoculation. To further complicate matters, plasmid copy numbers are often ambiguous...

  10. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    ... plasmid page is nested in. You can see in the example above that the bread crumbs trail starts in the...publication. Addgene makes this easy by providing a template citation for you to use in your Materials and ... Use the Addgene catalog number to easily find template citations for Addgene plasmids you have used in...  These sequences are provided in FASTA format (example) and can be analyzed using Addgene’s Analyze Sequence...Price of course! The price of some items vary, for example, plasmid kits are avaliable for less than than ...

  11. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    ...incubated together without forming large oligomeric complexes. Antibodies are also too large to be expressed...primary antibody-nanobody mix can be added to your sample, thus eliminating the secondary antibody incubation...two-step staining with antibodies. Nanobodies also simplify multicolor staining experiments because they don...568, or Alexa Fluor 647 before application to the sample. Staining with this multi-color staining workflow...imaging, rapid epitope-mapping and native protein complex isolation. eLife. PubMed PMID: 26633879 PubMed ...

  12. Transferable Skills Guide: Identifying Your Transferable Skills

    Type
    Blog Post
    ...transferable skills you possess because any potential employers, collaborators, or co-workers want to know that...socks, persevered through a nerve wracking commute complete with a disabled train, and now you’re sitting ...sitting in the lobby of (hopefully) your future employer. Your heart is pounding, you’re short of breath, and...my wheelhouse”, take a second to reflect. For example, say your boss approaches your team with a project...these responsibilities. If you are in charge of completing many meticulous protocols or tasks, you’ve probably...

  13. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    ...engineered DNA-binding molecule such as the CRISPR complex is expressed in cells to be analyzed, the Fujii...another "in vitro" form. In this form, the CRISPR complex, consisting of a recombinant dCas9 protein and ...interest with dCas9 and a targeting gRNA. One such example by the Xu lab is CRISPR affinity purification in...purification of a specific genomic region is another example of the many tools based on targeting of a genomic...genomic locus through a specific gRNA-dCas9 complex. When combined with next-generation sequencing, this ...

  14. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    ...editing, base editing, PACE, and DNA-templated synthesis are four examples of technologies pioneered in his...lockdown for weeks to months, pausing or terminating employment for a significant fraction of the workforce. ...between Jan. 1 and Mar. 1, 2020. Figure 2: A simple approximation of the effect of COVID-19 on data...effects fairly well. However, we observed one complication in the China data – there is a period of 1 month...

  15. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type
    Blog Post
    ...including depositing labs and institutions. For example, search for “Melina Fan” and you’ll be able to ...plasmids from a paper have been deposited? In this example we’re going to be looking for recombinant monoclonal...each filter is displayed in parentheses.  In this example I wanted to find a lentiviral plasmid to express... filters you do not need to start a new search. Simply click the ‘X’ next to any filter to clear it. ... plasmids that may be difficult to define for a simple search. These pages provide helpful information...

  16. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    ... the start, or if you can begin simply and build up to more complexity if needed. And since we live in...socioeconomic status and geographic location. For example, not all social media platforms are available or...expertise needed can increase exponentially with complexity. Podcasts Medium Easy for audience to engage...Written works Low Flexible. Low barrier to entry. Ample distribution channels both digitally and physically...

  17. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

    Type
    Blog Post
    ...experimental purposes. But what now? MoClo really simplifies vector construction by combining restriction ...process to produce a multigene construct. Figure exemplifies the extraction and use of a coding sequence of...four-base overhangs flanking each genetic part. (A) Example Level 0 assembly. BpiI recognition sites and four-base... Level 0 acceptor (bottom) for CDS1 parts. (B) Example Level 1 assembly. The choice between different ... MoClo user, but beginners or those performing complex assemblies should screen a minimum of 8–10 colonies...

  18. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...#211756 (left panel) or #211757 (middle panel). Samples were also immunoblotted against vinculin as a loading...10.1016/j.neuron.2023.03.009. PMID: 37015225.  Simple and highly specific targeting of microglia with...further enhanced by packaging the virus as self-complementary AAV (scAAV). However, scAAV vectors have limited...combination with miR124T targeting sequences, provides a simple yet valuable tool for microglia research and broader...AAV plasmids here! Serrano, C., et al. (2023). Simple and Highly Specific Targeting of Resident Microglia...

  19. Hot Plasmids - November 2023

    Type
    Blog Post
    ...were most efficient at complex edits, such as long or highly structured templates. Cas9 variants PE6e–g...PE6b was very efficient for short, unstructured templates and significantly reduced undesired edits (indels...also make CAR-T cell therapy more accessible by simplifying the production process and could even enable ...tet-responsive element (TRE) or the cFos promoter. For example, neurons treated with a phorbol ester activator...

  20. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    ... relevant bases within the editing window, for example all C’s will edit to T’s. Thus, it’s important ...modifiers available. This system utilizes an RNA template containing the desired edit adjoined to the sgRNA...knocking out a particular gene isn’t feasible. For example, temporary repression or knockdown allow you to... target genes using dCas9-based activators The simplest of the dCas9 activators consists of dCas9 fused... proteins. The Synergistic Activation Mediator complex, generally the strongest activator, consists of...
Showing: 441 - 460 of 938 results