We narrowed to 563 results for: pac

1. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

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   Blog Post

   ...These scaffolds are stretches of non-coding RNA interspaced with linkers that can fold into a secondary structure...

2. Advice for Moving Into Sales After Your Science Postdoc

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   Blog Post

   ...business of western blots and cloning, I decided to pack it in. I had just started month 8 of my 3 year Post-Doctoral...

3. Mentoring for Scientists: I Have a Mentor, What Now?

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   Blog Post

   ...Choosing a Good Mentor", and more! Check Out Angela Depace's Talk on "Precision and Plasticity in Mentoring...

4. Using CRISPR/Cas9 to Edit Disease Out of the Genome

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   Blog Post

   ... difficult to use larger enzymes. To reduce the impact of off-target mutations, scientists are using sgRNA...

5. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

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   Blog Post

   ...beyond. These new iGluSnFR3 variants come in AAV packaged virus aliquots being deposited at Addgene. Other...

6. Virtual Science Conference Coming Up? Three Addgenies Share Their Experience

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   Blog Post

   ... an on-site conference, you really have to make space for it,” says Bachle. “On the flip side, you can...

7. 25 Work From Home Tips for Scientists

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   Blog Post

   ... our mindfulness group is sticking with their Headspace practice even while working remotely. Social distancing...

8. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

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   Blog Post

   ...type of antibodies from an Andean mammal called alpaca could neutralize the SARS-CoV-2 virus. But while...

9. Plasmids 101: NGS Quality Control for Pooled Libraries

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   Blog Post

   ...commonly uses a custom script derived from the count_spacers.py file from Feng Zhang’s laboratory. There are...

10. Pushing the Limits of DNA Assembly

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    Blog Post

    ...placed. With other techniques, when mutations are spaced farther apart, it becomes hard to accurately execute...

11. Visualizing Translation at the Single Molecule Level

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    Blog Post

    ...s important to verify that these changes do not impact translation rate, as the authors of these papers...

12. Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

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    Blog Post

    ... systems in Drosophila: A synthesis of time and space, Trends Genet. 20, 384–391 (2004). PubMed PMID: ...

13. Addgene Depositors Get More Citations

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    Blog Post

    ...0.75   125.3 In figure 2, you can see the full impact from using these weighted numbers. Note: this does...

14. Four Factors that Differentiate the Stem Cell Field

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    Blog Post

    ...biology where questions of ethics can have profound impacts on the development of applications with the potential...

15. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

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    Blog Post

    ... Targeting three dCas9 molecules to a precisely spaced region allowed sequence replacement rates of approximately...

16. How to Lead a Great Meeting

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    Blog Post

    ... include every person who could potentially be impacted by a project. Instead, invite the experts on the...

17. Advice for Scientists Starting a Lab

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    Blog Post

    ... environment? Tom Ellis was offered a start-up package that included a postdoc and a PhD student in his...

18. Hot Plasmids - May 2022

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    Blog Post

    ...believes that this system could potentially be packaged into an AAV system. dCas9-SSAP will make the editing...

19. Antibodies 101: Isotypes

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    Blog Post

    ...structure, which means each IgM molecule has a binding capacity or valency of 10 antigens. An extra constant domain...

20. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

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    Blog Post

    ...violet-excited GFP variant Vex. To understand the impact of this gene editing tool on the immune system,...