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...open-reading frame and separated from the protein of interest by a 2A self-cleaving peptide (Goedhart et al,...correlation between the fluorescent protein of interest and the reference protein (Goedhart et al, 2011...

...RNA (gRNA) used to target the genomic locus of interest. As these off-target effects can be genome-wide...can allow for targeting of your genomic loci of interest that may not be accessible by wild-type Cas9, ...

...tissue types, recombinases allow you to further restrict gene expression to areas in which the recombinase...expression patterns, thus allowing you to further restrict gene activation to cells expressing both Cre and...

...and then unfollow those who do not capture your interest during that week.” You can also find Dr. Knoepfler...consistently engage with others about your topic(s) of interest. Hopefully through these links, I’ve: 1) convinced...

...about the various posters I visited. If you’re interested in neuroscience but are afraid you don’t have...virus for you. Neuroscientists are particularly interested in using AAVs because they provide a highly versatile...

...the intent is to make a knockout in your gene of interest, as it is prone to generating indel errors. Indel...bloggers!   David Wyatt is a graduate student interested in determining how the structure of broken ends...

...along with an antibody targeting the protein of interest led to rapid, targeted degradation that is dependent...independently control expression of your genes of interest. These transcription factors consist of the GAL4...

...s start with the paper itself: Ma et al. were interested in correcting an autosomal dominant mutation ... vs repair from the maternal MYBPC3 allele. Interestingly, they found that the embryos underwent repair...

...growth factors or serum to stimulate a pathway of interest, but there can be a lot of static since other ...inserting pdDronpa domains into loops of a protein of interest. If you plan to try this, it’s important to check...

...assay with your chosen FP in your cell type of interest before starting your experiments.  Selecting a...this fluorescent protein to tag your protein of interest will be problem-free. In figure 1 we provide an...

... approach to simultaneously clone two genes-of-interest (GOI) into two independent expression cassettes...pUC57-L3L2) that offer the advantage of using restriction digest cloning to introduce the GOIs into the...

...considered "natural tumor suppressors." A growing interest in the cancer microenvironment Ever since Stephen...Viva" in Aveiro, Portugal. She is particularly interested in science journalism and writing about biomedical...

...every project, time, attention, and/or length restraints mean you will have to pick and choose what concepts...feedback in a variety of ways, like having them restate or summarize the concepts you’re covering. Don'...

... allows synthetic biologists to use Type IIS restriction sites to easily assemble multiple DNA fragments...dimerize, bringing each terminus of Cre together to restore Cre activity. As soon as red light or PCB is no...

...When we developed this tool, there were so many interesting questions that we could apply [it to].” Using...Pharmacology at Novartis Oncology and is particularly interested in applying what we have learned about tumor ...

...the G receptor. In neurons, the G receptor is restricted to presynaptic nerve terminals (Lafon 2005). ...applications Use of G deleted RABV in conjunction with restricted TVA and G protein expression is a powerful development...

...plasmids are also used to teach students about restriction mapping and western blotting. Students who participate...genetics at Boston College. She is particularly interested in strategies to incorporate research into undergraduate...

... CRY2 and CIB1 domains to separate proteins of interest, a researcher can cause them to interact by stimulation...reversible oligomerization when fused to a protein of interest. Fusion protein oligomerization happens within...

...SpCas9. gRNA A gRNA targeting a DNA sequence of interest is expressed in 293T cells and loaded into Nanoblades...transduction for most cell types they tested. Interested in using Nanoblades? Plasmids are available from...

...particles are delivered into the brain region of interest. AAV or lentivirus?  Both AAV and lentiviral vectors...however, the spreading of vector to the area of interest may be very limited with this method, especially...