We narrowed to 759 results for: rest
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  TypeBlog Post...understanding how, when, and where TFs work is of broad interest to the scientific community. Recently, the Zhang... The TF map is a valuable resource for anyone interested in cell differentiation programs. The Zhang lab...
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With Our New Viral Service, We're Taking CRISPR Further!
TypeBlog Post...You can also enhance expression of your gene of interest with a variety of Cas9 activators available in...model that reflects the underlying biology you're interested in studying. You need to make sure your assay... - 
  
Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish
TypeBlog Post... Professor in Microbiology, and her group are interested in understanding the mechanisms behind Mycobacterium... Julio Ernesto Valdivia Silva is a master of interesting combinations: biomedicine and astrobiology. During... - 
  
"Build Your Plasmid": The Game - Play to Teach
TypeBlog Post...governed how this game was played. These rules restricted which cards could be played when and, depending...founder and consultant at FRESCI particularly interested in advanced in vitro models technology. Follow... - 
  
Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity
TypeBlog Post...RNA (gRNA) used to target the genomic locus of interest. As these off-target effects can be genome-wide...can allow for targeting of your genomic loci of interest that may not be accessible by wild-type Cas9, ... - 
  
New and Upcoming Viral Vectors - May 2020
TypeBlog Post...tissue types, recombinases allow you to further restrict gene expression to areas in which the recombinase...expression patterns, thus allowing you to further restrict gene activation to cells expressing both Cre and... - 
  
A Deep Dive into BioBuilder
TypeBlog Post...partnership with high school teachers to boost interest and understanding around the engineering of biology...by emailing [email protected]. Of particular interest this fall: The BioBuilderClub BioBuilderClub ... - 
  
A Practical Approach to Choosing the B(right)est Fluorescent Protein
TypeBlog Post...open-reading frame and separated from the protein of interest by a 2A self-cleaving peptide (Goedhart et al,...correlation between the fluorescent protein of interest and the reference protein (Goedhart et al, 2011... - 
  
Early Career Researcher Toolbox: Social Media for Scientists
TypeBlog Post...and then unfollow those who do not capture your interest during that week.” You can also find Dr. Knoepfler...consistently engage with others about your topic(s) of interest. Hopefully through these links, I’ve: 1) convinced... - 
  
A Novice's Perspective on Neuroscience - SfN2017
TypeBlog Post...about the various posters I visited. If you’re interested in neuroscience but are afraid you don’t have...virus for you. Neuroscientists are particularly interested in using AAVs because they provide a highly versatile... - 
  
CRISPR 101: Non-Homologous End Joining
TypeBlog Post...the intent is to make a knockout in your gene of interest, as it is prone to generating indel errors. Indel...bloggers! David Wyatt is a graduate student interested in determining how the structure of broken ends... - 
  
Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins
TypeBlog Post... approach to simultaneously clone two genes-of-interest (GOI) into two independent expression cassettes...pUC57-L3L2) that offer the advantage of using restriction digest cloning to introduce the GOIs into the... - 
  
The Breast Cancer Microenvironment: A Tumor’s Backstage Team
TypeBlog Post...considered "natural tumor suppressors." A growing interest in the cancer microenvironment Ever since Stephen...Viva" in Aveiro, Portugal. She is particularly interested in science journalism and writing about biomedical... - 
  
Human Germline Editing Using CRISPR
TypeBlog Post...s start with the paper itself: Ma et al. were interested in correcting an autosomal dominant mutation ... vs repair from the maternal MYBPC3 allele. Interestingly, they found that the embryos underwent repair... - 
  
Lighting Up Cell Signaling with Photoswitchable Kinases
TypeBlog Post...growth factors or serum to stimulate a pathway of interest, but there can be a lot of static since other ...inserting pdDronpa domains into loops of a protein of interest. If you plan to try this, it’s important to check... - 
  
Choosing the B(right)est Fluorescent Protein: Aggregation Tendency
TypeBlog Post...assay with your chosen FP in your cell type of interest before starting your experiments. Selecting a...this fluorescent protein to tag your protein of interest will be problem-free. In figure 1 we provide an... - 
  
Summer SciComm Series: Cognitive Load
TypeBlog Post...every project, time, attention, and/or length restraints mean you will have to pick and choose what concepts...feedback in a variety of ways, like having them restate or summarize the concepts you’re covering. Don'... - 
  
Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics
TypeBlog Post... allows synthetic biologists to use Type IIS restriction sites to easily assemble multiple DNA fragments...dimerize, bringing each terminus of Cre together to restore Cre activity. As soon as red light or PCB is no... - 
  
Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools
TypeBlog Post...along with an antibody targeting the protein of interest led to rapid, targeted degradation that is dependent...independently control expression of your genes of interest. These transcription factors consist of the GAL4... - 
  
New Tool for Lineage Tracing: The ClonTracer Library
TypeBlog Post...When we developed this tool, there were so many interesting questions that we could apply [it to].” Using...Pharmacology at Novartis Oncology and is particularly interested in applying what we have learned about tumor ...