Plasmids 101: Modular Cloning
Type
Blog Post
...transcriptional units.
Individual parts are defined DNA sequences with specific functions. Most individual parts...
We narrowed to 538 results for: sequence
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Viral Vectors 101: An Introduction to AAV
TypeBlog Post... except ITRs (inverted terminal repeats – the sequences that flank the deliverable DNA) are removed, resulting... -
Developing Lab Management Software for Biology
TypeBlog Post...namespace collisions you may encounter: vector, sequence, insert, trait, factor, expression, etc.There ... -
To Each HIS Own
TypeBlog Post... affinity chromatography against an amino acid sequence, or tag, on the protein. While a variety of methods... -
Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards
TypeBlog Post... outcome are then photoconverted, sorted, and sequenced to identify the mutants for further enrichment... -
Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options
TypeBlog Post...or chemogenetic receptor, through an IRES or 2A sequence. In this case, the proteins are not fused. The... -
GPCRs: How Do They Work and How Do We Study Them?
TypeBlog Post...protein-coupled receptor genes from the human genome sequence. FEBS Letters 520:97–101 . https://doi.org/10.1016... -
Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors
TypeBlog Post...is to knockdown a gene with RNAi. Here, a short sequence targeting the gene is cloned into a viral shRNA... -
Adeno Associated Virus (AAV) for Cell and Gene Therapy
TypeBlog Post... incurable. Rapid advances in next generation sequencing technologies have allowed scientists to quickly... -
Plasmids 101: The Wide World of Natural Plasmids
TypeBlog Post...classifications are still being created as whole-genome sequencing becomes more relevant (Garcillán-Barcia et al... -
Fluorescent Proteins 101: Introduction to FRET
TypeBlog Post...are often intramolecular probes, as the linker sequence between the donor and acceptor is sensitive to... -
Tips for Technical Support Calls
TypeBlog Post...technique or experiment, but a picture of a gel or a sequencing trace file can go a long way in helping us understand... -
Corralling and Cornering the Goldilocks Antibody
TypeBlog Post...relatively new technology that takes advantage of DNA sequencing to turn mAb clones into expression constructs... -
Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation
TypeBlog Post...interest. Having the CpG directly inside the gRNA sequence is a great guarantee that DNMT1 will be blocked...proteins and result in unclear transcriptional consequences (Sprujit 2013). Enzymatic domains tethered to... -
Plasmids 101: Optimizing Plasmid Yields
TypeBlog Post...more than enough DNA to verify your plasmid via sequencing or restriction digest. Chances are, you’ll even... -
Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015
TypeBlog Post...tRNAs much thought. High throughput transcriptome sequencing methods including work done by the ENCODE project... -
15 Hot Plasmids from 2017
TypeBlog Post...develop five new base editors with distinct PAM sequences, expanding the number of available target sites...Knockdown library. Although B. subtilis has a fully sequenced genome, many applications in biotech, and is considered... -
Technical Design of a Western Blot
TypeBlog Post...variation. Confirmation of sequence can be done from relatively cheap plasmid sequencing. While rAbs are monoclonal... -
Open Enzyme Collection
TypeCollection...to amplification, enzymes for cutting protein sequences to remove unwanted parts, and DNA-binding proteins... -
Hot Plasmids February 2024
TypeBlog Post...tags we know and love, the FLAG tag (amino acid sequence DYKDDDDK) may be one of the most versatile. Whether...