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We narrowed to 838 results for: tat

Showing: 441 - 460 of 838 results

  1. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    ...your messaging and your expectations of your audience, and their expectations of you, consistent and clear... racial/ethnic background, patient or caregiver status, geographic location, sense of humor, media consumed...

  3. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    ... Nucleic acid based machines Recombinase-based State Machines Enable Order-dependent Logic in vivo Nathaniel...received by a cell. These so-called recombinase-based state machines could potentially be used to report upon...this post to learn more about recombinase-based state machines and how they function. Adapting Toehold...

  4. Mentoring for Scientists: I Have a Mentor, What Now?

    Type
    Blog Post
    ...journal will help a Mentee track progress, adjust expectations, and record milestones in reaching goals. The...that I try to exercise every morning, but the temptation to skip the gym is strong. There are a few women...with regular accountability checks. Focus on implementation of your goals and respect the time your Mentor...

  5. DAP arrays for multiplex gene editing

    Type
    Blog Post
    ...Now with the DAP array, it is possible to use the state-of-art base editor, prime editor, or any CRISPR-...CRISPR-based gene editors, for correcting mutations or installing functional genes to study and potentially...).   Fig. 2: Comparison of previous representative dual-gRNA architectures with DAP array ...

  6. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

    Type
    Blog Post
    ...  The iodine-modifications also shift MG-2I’s excitation wavelength to 666 nm, which is within the near-infrared...possible to do things like in vivo imaging.   Excitation with NIR light also produces less non-specific... non-specific ROS when exposed to YFP and RFP excitation wavelengths, which suggests that it could be ...

  8. Science Career Options

    Type
    Blog Post
    ...Manager Patent Lawyer Science Market Research Statistician   Attention to detail Patience, accuracy ...Computer and writing skills Writing a thesis or dissertation demonstrates that you have advanced writing ...including: Science Reporter Technical Writer Statistician Bioinformatician Science Writer Grant Writer...

  9. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...harnessed for stable delivery of various genes, mutations, or treatments into cells, and has been widely...possible development of cancer. Pseudotyping Tropism dictates which types of host cells the gamma-retroviral...

  10. Technical Design of a Western Blot

    Type
    Blog Post
    ...than indirect ones and can be used in highly quantitative assays. On the other hand, they are not as sensitive...stability Limited protein size range Tris-acetate 40–500 kDa Tris-tricine 150 V, 1–3 hours ...detection. Film also needs to be digitized for quantitative work — and with the advent of online lab notebooks...high-abundance single protein controls in semi-quantitative immunoblotting. Journal of Neuroscience Methods...

  11. 15 Hot Plasmids from 2017

    Type
    Blog Post
    .... While certain cell types, such as principal excitatory cells and glia, have been successfully targeted...researcher’s locus of interest. NHEJ- or HDR-induced mutations in ATP1A1 can render cells resistant to the small...improves editing efficiency. Importantly, the point mutations created by Agudelo et al. prevent ouabain binding...DNA molecular weight markers The Tan lab at Penn State - set out to “rule” Molecular Biology. Under the...

  12. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    ...this one mutated methionine was not enough to prevent alternative initiation, so they mutated the methionines...the target site (the same sequence you’d like to mutate in the genome) and an out-of-frame H2B (histone...

  14. Why and how to start studying chromatin (with Addgene's help)

    Type
    Blog Post
    ...megabase away, or looking at the histone methylation status at a double-strand break that needs repairing. ...start sharing your plasmids through Addgene, don’t hesitate to start the process! Addgene depositors who study...linked below are partially curated from the presentations and posters I saw at the 2018 Keystone Chromosome...

  15. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    ...promoters, which can be switched from an OFF to an ON state, and how you might use these in your research. When...negative control. Positive inducible In the OFF state, the promoter is inactive because the activator ...transcription.     Negative inducible In the OFF state, the promoter is inactive because a bound repressor...

  16. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    ...to neuroscience prior to this conference was a rotation in Qiao Zhou’s lab in graduate school where I ...neuroscience and you don’t have a background in statistics and programming, you’d be wise to start working...and brain monitors. Walking around the poster presentation area, I honed in on projects using biological...

  17. Hot Plasmids - May 2022

    Type
    Blog Post
    ...long sequences with reduced risks of unwanted mutations in mammalian cells. Long sequences traditionally...traditionally have higher risks of off-target mutations. These plasmids contain catalytically inactive dCas9 and...challenge due to multiple factors, including limitations of number of flourescent proteins that can be...

  18. Antibodies 101: ChIP

    Type
    Blog Post
    ...Chromatin immunoprecipitation (ChIP) is an extremely useful technique that provides insight into protein...to counteract the effects of increased sample agitation; a shorter sonication time could be an opportunity...resources on Addgene’s blog Antibodies 101: Immunoprecipitation  Antibodies 101: Polyclonal Antibodies Antibodies...

  19. Hot Plasmids - February 2022

    Type
    Blog Post
    ...Cas12a for multiplexed gene perturbation The Penn State Protein Ladder Engineered miniature CRISPR-Cas system...2021. doi: 10.1038/s41587-020-0600-6. The Penn State Protein Ladder  by: Rachel Leeson This simple, inexpensive...expression and purification. Figure 3: The Penn State Ladder run on SDS-Page gels. A: individually expressed...

  20. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    ... efficient. Pro-Tip Make sure to reverse the orientation of the crRNA’s direct repeats when expressing...preventing packaging of the virus.  Reversing the orientation of the direct repeat protects the (+) stranded...cells, but notice how Zetsche et al reverses the orientation of the promoter driving expression of the crRNA...
Showing: 441 - 460 of 838 results