Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 846 results for: tat

Showing: 441 - 460 of 846 results

  2. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    ...Historically, glutathione-S-transferase (GST) fusion proteins have been immobilized on a reduced glutathione matrix...protein-ligand interaction approaches that of GST-glutathione. Biotin-based immobilization for kinetic analysis...

  3. Plasmids 101: Origin of Replication

    Type
    Blog Post
    ...negative regulation and can be manipulated with mutations in the replicon. For example, the pMB1 ori maintains...per cell, while pUC – which differs by only two mutations – will produce as many as 700 copies per cell.... numbers since the bacteria have just reached stationary phase, but the cells have not started to die ...

  4. 5 Uncommon Ways to Gain Career Skills During an Uncommon Time

    Type
    Blog Post
    ...calls, how about trying your hand at a video presentation? Most video platforms make it easy to record...get their input as well. Giving a great video presentation is going to be a useful skill for the foreseeable... Science: Where to Search for Funding The Ohio State University; Graduate Student Funding Opportunities...

  5. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    ... Nucleic acid based machines Recombinase-based State Machines Enable Order-dependent Logic in vivo Nathaniel...received by a cell. These so-called recombinase-based state machines could potentially be used to report upon...this post to learn more about recombinase-based state machines and how they function. Adapting Toehold...

  6. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    ...your messaging and your expectations of your audience, and their expectations of you, consistent and clear... racial/ethnic background, patient or caregiver status, geographic location, sense of humor, media consumed...

  7. Mentoring for Scientists: I Have a Mentor, What Now?

    Type
    Blog Post
    ...journal will help a Mentee track progress, adjust expectations, and record milestones in reaching goals. The...that I try to exercise every morning, but the temptation to skip the gym is strong. There are a few women...with regular accountability checks. Focus on implementation of your goals and respect the time your Mentor...

  8. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

    Type
    Blog Post
    ...  The iodine-modifications also shift MG-2I’s excitation wavelength to 666 nm, which is within the near-infrared...possible to do things like in vivo imaging.   Excitation with NIR light also produces less non-specific... non-specific ROS when exposed to YFP and RFP excitation wavelengths, which suggests that it could be ...

  10. Science Career Options

    Type
    Blog Post
    ...Manager Patent Lawyer Science Market Research Statistician   Attention to detail Patience, accuracy ...Computer and writing skills Writing a thesis or dissertation demonstrates that you have advanced writing ...including: Science Reporter Technical Writer Statistician Bioinformatician Science Writer Grant Writer...

  11. DAP arrays for multiplex gene editing

    Type
    Blog Post
    ...Now with the DAP array, it is possible to use the state-of-art base editor, prime editor, or any CRISPR-...CRISPR-based gene editors, for correcting mutations or installing functional genes to study and potentially...).   Fig. 2: Comparison of previous representative dual-gRNA architectures with DAP array ...

  12. Technical Design of a Western Blot

    Type
    Blog Post
    ...than indirect ones and can be used in highly quantitative assays. On the other hand, they are not as sensitive...stability Limited protein size range Tris-acetate 40–500 kDa Tris-tricine 150 V, 1–3 hours ...detection. Film also needs to be digitized for quantitative work — and with the advent of online lab notebooks...high-abundance single protein controls in semi-quantitative immunoblotting. Journal of Neuroscience Methods...

  13. 15 Hot Plasmids from 2017

    Type
    Blog Post
    .... While certain cell types, such as principal excitatory cells and glia, have been successfully targeted...researcher’s locus of interest. NHEJ- or HDR-induced mutations in ATP1A1 can render cells resistant to the small...improves editing efficiency. Importantly, the point mutations created by Agudelo et al. prevent ouabain binding...DNA molecular weight markers The Tan lab at Penn State - set out to “rule” Molecular Biology. Under the...

  14. Fluorescent Proteins 101: Luciferases

    Type
    Blog Post
    ...proteins. On the other hand, they don’t require an excitation light source (usually a laser in fluorescence...to a nearby fluorophore, which emits light. No excitation illumination needed — just the luminescent substrate... proteins to be visualized simultaneously. Complementation systems for examining protein-protein interactions...

  15. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    ...there is a classification system, CasID, to help facilitate direct comparison of different enzymes. CasPEDIA... their sequence (NGG, etc.) as well as their orientation relative to the gRNA (5’ or 3’). In some cases...would be helpful to know for viral packaging limitations. Maybe you want to read literature on the less...

  16. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    ... efficient. Pro-Tip Make sure to reverse the orientation of the crRNA’s direct repeats when expressing...preventing packaging of the virus.  Reversing the orientation of the direct repeat protects the (+) stranded...cells, but notice how Zetsche et al reverses the orientation of the promoter driving expression of the crRNA...

  17. Antibodies 101: ChIP

    Type
    Blog Post
    ...Chromatin immunoprecipitation (ChIP) is an extremely useful technique that provides insight into protein...to counteract the effects of increased sample agitation; a shorter sonication time could be an opportunity...resources on Addgene’s blog Antibodies 101: Immunoprecipitation  Antibodies 101: Polyclonal Antibodies Antibodies...

  18. Hot Plasmids - February 2022

    Type
    Blog Post
    ...Cas12a for multiplexed gene perturbation The Penn State Protein Ladder Engineered miniature CRISPR-Cas system...2021. doi: 10.1038/s41587-020-0600-6. The Penn State Protein Ladder  by: Rachel Leeson This simple, inexpensive...expression and purification. Figure 3: The Penn State Ladder run on SDS-Page gels. A: individually expressed...

  19. Why and how to start studying chromatin (with Addgene's help)

    Type
    Blog Post
    ...megabase away, or looking at the histone methylation status at a double-strand break that needs repairing. ...start sharing your plasmids through Addgene, don’t hesitate to start the process! Addgene depositors who study...linked below are partially curated from the presentations and posters I saw at the 2018 Keystone Chromosome...

  20. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    ...this one mutated methionine was not enough to prevent alternative initiation, so they mutated the methionines...the target site (the same sequence you’d like to mutate in the genome) and an out-of-frame H2B (histone...
Showing: 441 - 460 of 846 results