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We narrowed to 655 results for: EPO

Showing: 231 - 240 of 655 results
  1. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    ...the sequencing results of the plasmids in our repository with known reference sequences, such as full ...plasmid sequences provided by the laboratories that deposit their plasmids with us or other entries in NCBI...NCBI’s numerous databases. As our repository has grown over the years (we now have over 60,000 plasmids!...
  2. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    ...research uses ancestry groups rather than self-reported race, it highlights the myriad issues researchers...designed a tool that could analyze CRISPR sgRNAs and report the likelihood of any given sgRNA having an ancestral...complicating the issue is the long and ugly history of reporting false essentialist links between biology and ancestry...
  3. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    ...pathway is ubiquitous, with successful recombination reported in all laboratory E. coli strains tested so far...use of this pathway as a cloning tool was first reported over 25 years ago (Douglas and Howard, 1991, Bubeck...exploiting bacterial in vivo assembly." Scientific reports 6 (2016): 27459. PubMed PMID: 27264908. PubMed ...
  4. MXS Chaining

    Type
    Blog Post
    ...produce complex fluorescent reporter constructs. These fluorescent reporters can be used to detect structure...protein sequences to develop diverse fluorescent reporters is a useful application of high-throughput cloning...
  5. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    ...vector with a reporter, such as a fluorescent protein, to visualize vector uptake. These reporters might be...t common for simple transfection experiments. Reporters Finally, in some cases a researcher may choose...
  6. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    ...collaborative team from the David Liu and Dali Li labs reported a similar tool shortly thereafter, using mouse...substrates. The HuidaGene Therapeutics team has deposited their editors, called gTBE and gCBE, with Addgene...Transversion Editors Read about four base editor reporters Resources on Addgene.org Browse CRISPR plasmids...
  7. Plasmids 101: Biotinylation

    Type
    Blog Post
    ...primary antibody respectively. Avidin bound to a reporter is then added to the reaction for easy detection... experiments. During detection, a biotinylated reporter enzyme that is pre-incubated with free avidin ...protein of interest leading to amplification of the reporter signal and easier detection. This strategy is ...
  8. Hot Plasmids: Fall 2025

    Type
    Blog Post
    ...plasmids, antibodies, viral preps, and more in the repository through our Hot Plasmids articles. Here's what...Grelet Lab developed MitoTRACER, a retroviral-based reporter system that permanently labels the cells receiving...) under CC BY license. With 143 plasmids deposited from this paper, a large menu of tool and control...
  9. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    ... Team is responsible for guiding new deposits into the repository, performing quality control, and providing...kit creation and quality control efforts with depositors; Gizela, the lab member who knows how the physical...
Showing: 231 - 240 of 655 results