We narrowed to 997 results for: lars;

... Laboratory of Molecular Biology. Plasmid cloning is an essential part of any molecular biology project...the MRC Laboratory of Molecular Biology. He is currently investigating the molecular biology of synaptic...for plasmid cloning Find our molecular biology reference here Find molecular biology protocols here  ...current cloning techniques involve the assembly of a circular plasmid in vitro, before transforming it into ...transformed into E. coli and plasmid assembly (re-circularization) occurs in vivo. We use XL-10 Gold E. coli,...Javier García-Nafría from the MRC Laboratory of Molecular Biology. Jake Watson is a postdoctoral researcher...investigator scientist at the MRC Laboratory of Molecular Biology. He is interested in neuronal signal transduction...

...cells. They bind to proteins on the surface of extracellular pathogens such as parasites or microbes, or ...(Cruse et al., 2004). Because proteins are much larger than this, it may contain many epitopes that antibodies...antibodies are less likely to cross-react with other, similar proteins due to recognizing a single epitope of...monoclonal antibodies here. Recombinant antibodies Similar to monoclonal antibodies, recombinant antibodies...proteins. The proteins are separated based on molecular weight by SDS-PAGE, and are subsequently transferred...internal control for Western blots, shows that a similar concentration of cell lysate was added to each ...   Enzyme-linked immunosorbent assay (ELISA) Similarly, ELISA is used to detect the presence of a single...

...guest blogger Pooran Dewari, a postdoc in Steve Pollard’s lab at the MRC Centre for Regenerative Medicine...workhorses of the cell – never work alone in the cellular milieu. It is, therefore, critical to understand...biochemical tasks in the cell. One of the most popular approaches to study protein interactions is the...spectrometry to identify the interacting proteins. Similarly, in ChIP-seq assays, chromatin fragments that ...ambitious projects that require epitope tagging of a large cohort of genes (e.g. tagging genes representing...success of the epitope tagging experiment depends largely on how efficiently these components are delivered... TAG-IN bioinformatics tool.A recent article by Clarke et al shows that crRNAs binding to the template...

...fingertips the tools to probe cellular dynamics and function with extraordinary molecular detail.   References...offer scientists a simple yet powerful way to tag cellular proteins and investigate protein localization,...fluorescence when illuminated with blue light (1). Similar to PA-GFP, scientists later developed red fluorescent...photoactivatable fluorescent protein for your experiment Similar to performing experiments with standard FPs, before... chimeric protein will oligomerize, disrupting cellular function and localization, or leading to protein...to optimize your activation protocol for your particular set up. Applications of photoactivatable fluorescent...live imaging of proteins, organelles, and cells. Similar to FPs, PA-FPs allow for non-invasive labelling...

... dcm: Similarly, strains with this mutation are unable to methylate cytosine within a particular sequence...blog, we reviewed the salient features of several popular strains of E. coli for DNA propagation. While great...recombinant protein expression and some of the most popular commercial strains designed to avoid them. Why...products may be toxic to the host when expressed, particularly those that are insoluble, act on DNA, or are...is completely deleted (designated lon or Δlon) similary reduce proteolysis of the expressed proteins. ...coli T5 promoter (found on vectors such as pQE or similar), inducible under IPTG (lac repressor on the pREP4...from these strains is not recommended as these auxillary plasmids may be isolated during the prepping process...

...about $10 US dollars worth of glass powder pruchased from a pottery supply house.  These large, cheap aliquots...about energy conservation even before it was a popular topic in the media.  At one point I started to ... purifying cut plasmid DNA from agar slices.  Molecular biologists used this procedure daily before the...of techniques like PCR and DNA synthesis. Most molecular biologists bought the pricey stuff in 50 ul aliquots...science project started when I realized I was pouring large agarose gels for DNA visualization or isolation,...amount of agarose, I could pour a very thin gel on a large glass slide using only surface tension.  It's a ...despite the fact that my lab mates thought this was hilarious.  Birth of the baby gel box Finally, taking conservation...

...As an example, say you are considering either a cellular screen or a selection. A selection is a process...all known genes are assayed for their role in a particular capacity. Drug screens, on the other hand, are...suppressor screens These types of screens are very similar to forward and reverse screens, except that they.... Another example is using a mouse model of a particular disease as the input population. For these experiments... which are worth considering. RNAi screens are similar to CRISPR, but instead of knocking out the genes...thus not assayable with CRISPR. Another method, popularized in yeast, are temperature sensitive screens. ...temperature to exacerbate a mutant phenotype, very similar to enhancer/suppressor screens. Selections Selections...

... using the kit to target a large number of genes involved in cardiovascular and metabolic diseases. We...Regenerative Biology is to understand the basis for cardiovascular and metabolic human diseases. They do that ... Addgene: How do you use this kit to target a particular DNA sequence? Musunuru: The kit includes 832 ...been implicated in disease or, in cases where particular variants or mutations have been reported in people...of the genome editing process. The first really popular tool was the zinc finger nucleases. Unfortunately... much more reliable. If you design them for a particular sequence, there is a much better chance they ...power here is that you could potentially sequence a large number of individuals and find several mutations...

...readout for subcellular fractionations, a procedure that isolates proteins from different cellular compartments...protein levels relative to standards, detect the cellular location of proteins, and can be a readout for...addition to the samples, a protein standard of known molecular weight in a separate lane can help confirm the...migration resistance and improve separation. For large proteins, reduce the percentage of acrylamide. To... commonly used, PVDF tends to be slightly more popular due to its durability and high binding capacity... and typically takes less than an hour. However large proteins typically do not transfer as well using...could contain unintended cross-reactive proteins. Similarly, if you vary the experimental conditions from ...

...results. As biological researchers deal with larger and larger datasets and use computer based analysis techniques...the description to watch any sections you’re particularly interested in, but I’d recommend watching the...Reproducibility Project: Cancer Biology have produced similarly low rates of reproducibility. Failed attempts ...money with estimates reaching in the billions of dollars (Freedman et al 2015). Finally, as Pamela Hines... characterised antibodies against all human extracellular proteins with community-based validation. They...modified analyses on published data if you need to. Similarly, FigShare also makes it easier to share data through...on many different levels - it’s hard to process large amounts of information and, from a SciComm perspective...