We narrowed to 643 results for: ATO

1. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

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   ..., the stem-loop structure of transcriptional terminators. Another potential issue is sequence similarity...

2. Viral Vectors 101: Gamma-Retroviral Packaging Systems

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   ...Woodchuck hepatitis virus post‐transcriptional regulatory element; sequence that stimulates the expression...

3. Sharing is Caring: But How to Distribute Open Hardware?

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   ... in science brings stronger networking across laboratory and disciplinary boundaries and can allow for...

4. Plasmids 101: Screens vs. Selections

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   ...selected growth conditions. For screens, there is no obligatory growth advantage – that is, you are not specifically...

5. A Practical Approach to Choosing the B(right)est Fluorescent Protein

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   ...been used in yeast by Lee et al (2013) and in nematodes by El Mouridi et al (2017) and Heppert et al (...

6. Multiplex Genome Editing with CRISPR-Cpf1

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   ...multiplexing vectors are larger because they require regulatory sequences to allow for expression of multiple...

7. Top Requested Lentivirus and AAV of 2016

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   ..., Koda S, Ye C, Rogan SC, Adams AC, Cusher DS, Maratos-Flier E, Roth BL, Lowell BB. Rapid, reversible ...

8. Behind the scenes: Addgene’s new search engine and more

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   ...of other websites, we built our new search engine atop Elasticsearch, a powerful open-source search engine...

9. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

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   ...microRNAs (miRNAs) are recognized as important regulators of post-transcriptional gene expression across...

10. Antibodies 101: Monoclonal Antibodies

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    ...the antigen-binding sites of the antibody - the paratopes - may change and impact the specificity and avidity...

11. Communicating Your Science With Help From ComSciCon

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    ...in all STEM fields become excellent science communicators. To empower graduate students to become ambassadors...

12. Why and how to start studying chromatin (with Addgene's help)

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    ...Chromosome Organization. These aren’t the only investigators in the chromatin field who have deposited with...

13. Building and Validating Recombinant Antibodies: A Case Study

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    ...secondary antibodies are commonly used in research laboratories to detect the different subclasses, but there...

14. How to Make Friends and Meet People at a Scientific Conference

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    Blog Post

    ...You never know when you will meet a potential collaborator Some of these people you meet will review your...

15. Dos & Don'ts When Publishing a Scientific Manuscript

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    ...communities. The ARRIVE guidelines for the reporting of laboratory animals during in vivo research and the CARE ...

16. Bacterial Expression Systems

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    ...Two-Hybrid System Various Plasmids placO with lambda-C1 operator Beta-galactosidase Ann Hochschild Bacterial two-hybrid...

17. Addgene: The First Twenty Years

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    ...amazing years in front of us. So in our last celebratory moment, we wanted to walk through some of our...

18. Antibodies 101: Fab Fragments

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    ...secondary antibodies you already have in your refrigerator will not recognize a Fab fragment. In order ...

19. Antibodies 101: Conjugation

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    ...way they are, the research antibodies in your refrigerator often need a little help to be useful. Mainly...

20. Viral Vectors 101: An Introduction to AAV

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    ...make AAV ideal for human therapies and in vivo laboratory experiments ranging from transient gene expression...