We narrowed to 931 results for: TIM

...desired chimeric mice, you would breed them many times, hoping to produce a mouse with the desired edit...template with 55-100 bp homology on each side. For optimal repair, place the loxP sites precisely at the cut...

...important that the digest go until completion. The time required for complete digestion varies for different...speeds; after running the gel for some duration of time, these differently sized pieces will be in different...

...and vice versa? We draw a parallel between the timelines of the scientific advancements and the noteworthy...amino acid recognition sites had to be designed each time we wanted to target new genomic sites. The discovery...

...Class I TEs are considered replicative since every time they jump they make a copy of themselves. This increases...active in mammalian cells compared to the codon-optimized wild-type piggyBac transposase.  piggyBac plasmids...

...concentrate your lentiviral particles to get them to the optimum concentration to infect your cells of interest....References 1. Miller, A. DUSTY, and C. A. R. O. L. Buttimore. "Redesign of retrovirus packaging cell lines ...

...are the most efficient (i.e., happen most of the time). Figure 1 shows a graph from the BD® Spectrum Viewer...show efficient absorption at 650 nm, APC-Cy7's optimal excitation happens at higher wavelengths of about...

...This is not an extensive list Acceptor labeling: Sometimes it's okay to be wrong In contrast to FRET donors...PubMed PMID: 16908183. 10. Saraheimo, Satu, et al. "Time-Resolved FRET-Based Approach for Antibody Detection–A...

... a Western blot can be a bit daunting for first-timers. That's why we gave our written protocol a video...

...new sections, including our popular "Dimers and Multimers" post. It's also been re-organized and updated...

...techniques — but perhaps are a little limited on time — check out Addgene’s new Quick Tip series on YouTube...

...from producing your own vectors in the lab to optimizing AAVs in your experiment.      Our second edition...

...how you’re decorating your lab for the upcoming wintertime festivities! Help us spread some cheer by snapping...

...welcome to Depositor Week 2022! It’s that special time of year where we Addgenies use our social media ...

...how you’re decorating your lab for all upcoming wintertime festivities: Hanukkah, Christmas, ugly sweaters...

...person, we selected the finalist based on the first time of submission.)     We love the way the names...

...welcoming committee. However, the majority of the time was spent with Addgenies meeting each other's friends...

...the repository that have been shared over 140,000 times to over 75 countries. That’s a lot of CRISPR! As...

...conflict with our values. For this reason, our timeline has accelerated. We significantly reduced posting...

...Zinc Finger, TALE, CRISPR, and SynBio plasmids over time. You can see these data in the slide share. ...

... titer, serotype, injection site, and incubation time. Learn more about the Data Hub in this blog post...

... HQ and for some of us, that means spending more time with our pets. These new “co-workers” have made ...

...Ahem, ahem, drum roll please! It's time to announce the winners of our 2021 Deck the Lab Contest! Though...

...interested in biology, working in a lab for the first time, and thinking about the future. Many thanks to ...

...keep an eye on our blog and social media. In the meantime, thank you all so much for being part of our community...

... research labs, and then pivoted to writing full-time in mid-2020. As I left the ivory tower and walked...

...additional data including the subject’s age at the time of injection and how long the scientist waited after...

...will be removed before your account is charged.  Timelines  We have offered VSV and SARS-COV-1 plasmids under...

...Videos can save researchers time by quickly relaying key points about an unfamiliar topic or lab process...

...sure to come by booth #851 and say hello! Just in time for this meeting, Addgene is launching a new resource...

...search many different antibody catalogs at the same time.  If you're looking for an antibody, especially...