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We narrowed to 813 results for: cell

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Showing: 691 - 720 of 813 results

  6. Retrograde AAV viral preps

    Type
    Collection
    ... of Cre, and expresses NLS-EGFP in Cre-positive cells. Control Brandon Harvey 137164 pAAV-nEF-Con/Fon/...

  7. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    ...Guide RNA and Target DNA. Cell, 156(5), 935–949. https://doi.org/10.1016/j.cell.2014.02.001 Osuka, S., Isomura...efficiency and are important for robust activity in cells (Jiang & Doudna, 2017). Within an interior cavity...it, so many researchers think the enzyme needs cellular recycling factors to release its handiwork (Sternberg...

  9. Summer SciComm: Preprints

    Type
    Blog Post
    ...Lacy was also employed by the American Society for Cell Biology in a position working with preprints in ...

  10. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    ...expression in knock-out or knock-down down tissues and cell lines; orthogonal strategies that compare antibody... project aimed at understanding how a critical cellular pathway is regulated. You’ve read all the background...

  14. The Pleiades Promoter Project

    Type
    Collection
    ...therapies for brain disorders by enabling region- and cell-specific gene-delivery in the mouse brain through...

  15. Optogenetics AAV Preps

    Type
    Collection
    ...Optogenetics AAV Optogenetic tools can be used to control cell activity in living tissue with light. Optogenetics...

  16. Addgene: The First Twenty Years

    Type
    Blog Post
    ...Facebook and Gmail, were launched. And Melina Fan, a cell biology graduate student, was frustrated by the ...to receive the Massachusetts Nonprofit Network Excellence Award for our electronic MTA system. We also ...

  17. Antibodies 101: Fab Fragments

    Type
    Blog Post
    ...fragments for multiplexed super-resolution microscopy. Cell Rep Methods 2(10):100301. https://doi.org/10.1016...

  18. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    ...protein of interest in a complicated solution like cell lysate. But they can be a lot of work — and what...better than a nitrocellulose membrane. If you have already run your blot using nitrocellulose, you may decide...instructions for the membrane you are using. Dry nitrocellulose membranes are brittle, so proceed with caution...

  22. To Each HIS Own

    Type
    Blog Post
    ...This can be pretty complicated given that a single cell has tens of thousands of proteins functioning in...

  25. Lab Automation at Addgene

    Type
    Blog Post
    ...our set up. The cooling block is used to keep our cells from warming up too much and dying.  Also, we added...

  26. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    ...Precise and efficient scarless genome editing in stem cells using CORRECT. Nat Protoc 12, 329–354 (2017). DOI...

  27. 6 Tips for Grant Writing

    Type
    Blog Post
    ...Cambridge University where he studied mechanisms of cell division. Recently Seán got the entrepreneurial ...research (either because of expertise, equipment, or excellence of the institution). Likewise, if you’re staying...
Showing: 691 - 720 of 813 results