We narrowed to 990 results for: Abo

...results, and subsequent conclusions. Being vigilant about these errors is an important part of maintaining...

... to devise an experiment that can tell you more about its function.  Resources and references Resources...

...completely inert, but others can cause diseases. Nervous about handling viruses? That’s okay! In this article we...

...In a direct approach, that is simple - but what about when you need to use an indirect (primary and secondary...

... plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here.  Here's...

...drawback of this approach is that it can be quite labor intensive. Target cells are transduced with serial...

...synaptic events By Brian O’Neill The Lin Lab and collaborators recently created ASAP5, a faster and more responsive...

...dehydration (Howard & Harvey, 2017). You can learn more about common AAV applications by reading our Viral Vectors...

... al., 2023). We trained our models in close collaboration with the Krauthammer lab (University of Zurich...

...with Addgene by year. (C) Cumulative number of laboratories that have deposited CRISPR plasmids by year....

...especially essential. If you want to learn more about how viruses can be used for chemigenetics, biosensors...

...acid, suggesting that MPyV system can improve metabolic pathway yields by encapsulating a single enzyme...

... Fluorescent Protein 101 blog series Learn more about FRET Find protocols and tips for making plasmid ...

...Additional Resources on the Addgene Blog Learn More About Fluorescent Protein Aggregation Catch Up On Your...

...protein (GFP) (6).   The Dodt and Frank Bradke laboratories later developed the 3DISCO (3-dimensional imaging...

...flow-through solutions and use clean tubes? How about spin times, were these long enough?  There are, ...

...validated, it’s ready for use! You can learn more about common antibody applications in our Antibodies 101...

...this introduction gives a sense of what AdVs are about – and we hope that it inspires further use of existing...

...applications and there’s just not much to be done about that.  Second, expansion introduces distortions....

...dependencies associated with each ancestry group. Since about 90% of the cell lines used in DepMap CRISPR screens...

...asked why they don’t share, many express concern about sharing negative data they cannot explain, perhaps...

..., 2020), to multi-copy genomic integration of metabolic cassettes (Zhang et al. 2020); however, the technology...

...Microbiology, National Emerging Infectious Disease Laboratories.  She is interested in genome replication and...

...University of Tokyo, Osaka University). Can you speak about your different experiences in research labs. What...

...Collection and the BRAIN Armamentarium Collection, a collaborative effort that the Allen Institute contributes ...

...precise and accessible genome engineering. More elaborate protocols are available in our earlier publications...

...sequencing was considered the gold standard until about 2008 when a new, higher-throughput sequencing-by-synthesis...

...conjugate antibodies themselves, the process can be laborious and expensive. In some cases, conjugation methods...

...plasmids may have high copy numbers and reduced metabolic load (Meyer 2009). Figure 1: Idealized ...

...section of the Addgene antibody page, and learn more about the whole-brain reference atlas efforts, including...

...ordering a pre-made library, you don’t need to worry about amplifying and producing lentivirus. But pre-made...

...you enjoy playing with our genes. Have fun!    About the authors: Marjorie Buss is a PhD student studying...

.... Eugene Koonin and Kira Makarova, longtime collaborators of Omar and Jonathan and experts in CRISPR evolution...

... also toxic at high concentrations as described above and is inactivated by the complement system in serum...

...therapy. In 2014, RSRT brought together a strong collaboration between two gene therapy labs (Brian Kaspar ...

... Addgene.org Find All Available AAV Learn More about Virus Production Find Viral Vector Protocols ...

... & Corey, D. R. (2021). Reexamining assumptions about miRNA-guided gene silencing. Nucleic Acids Research...

...transcriptional silencing (Nuñez et al., 2021). Now, in a collaboration with the Vallabh/Minikel Lab, the Weissman Lab...

...limitations is the size of GFP: ~240 amino acids or about 28 kDa. GFP and its homologues have a beta-barrel...

... Addgene. In addition to the plasmids described above, Addgene carries several useful CRISPR toolkits ...