We narrowed to 1,100 results for: Ide
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TypeBlog Post... from IPI Simon Moe, from Promega, who wrote a guide to selecting fluorescent dyes and ligands. Illuminating...Controls Priyamvada Prathima, who wrote a helpful guide to understanding your flow data. Flow Cytometry...
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Celebrating the 2024 Nobel Prize in Chemistry
TypeBlog Post...awarded the Nobel Prize in Chemistry last week alongside Demis Hassabis and John Jumper! The Baker Lab ...like high resolution Ras sensors and crosslinked peptides proposed as a scaffold for a new generation of... -
Antibody Data Repositories and Search Engines
TypeBlog Post...share validation and experimental data to help you decide if the antibody is a good fit for your experiment...improving antibody quality throughout research, consider checking out the Only Good Antibodies community... -
New Podcast Segment: Hot Plasmids
TypeBlog Post...We’re breaking into more audio and video on the Addgene Blog and Addgene website. As we push forward ... a Graduate Lab and Project Check Out 5 Science Videos from Tom McFadden Resources at Addgene.org Sign... -
PEI Calculator for Planning AAV Packaging Transfections
TypeBlog Post...you plan PEI transfections for AAV packaging, considering using this PEI Calculator. AAV packaging typically...Additional Resources on the Addgene Blog Important Considerations When Using AAVs AAVs Are Great Tools for Gene... -
New Viral Vectors - Spring 2025
TypeBlog Post...the diverse needs of our community and support a wide range of experimental designs. Here are nine great... vectors? Browse our in-stock viral vectors or consider our AAV Packaged on Request service, which offers... -
Congratulations, Deck The Lab winners!
TypeBlog Post... their entire floor, and put together a festive video to showcase it all! View this post...symmetry. 🎶Walking in a Winter Wonderlab🎶 We decided to #DeckTheLab with virus snowflakes from @CVRHutchinson... -
Ghanaian Researchers to Receive Plasmid Awards from Addgene and Seeding Labs
TypeBlog Post... Addgene has been working with Seeding Labs to provide plasmid grants to researchers in developing countries...We are excited to discover what new and creative ideas applicants will propose for plasmids from the Addgene... -
Addgene Stands with the Scientific Community
TypeBlog Post...free educational resources: Our eBooks, videos, science guides, and protocols remain freely accessible... -
Synthetic Biology, Artificial Chromosomes, iGEM & More - Interview with Tom Ellis
TypeBlog Post...Benoit Giquel. Traveling from our offices just outside of London, it was a short journey for Benoit to...production. Chapter 5: The future of Synthetic Biology. Wide adoption of synthetic biology techniques in biology... -
Addgene's Website Update
TypeBlog Post...search bar will change to an easy-to-use depositing guide, with links to our most helpful depositing articles...choices informed by Web Content Accessibility Guidelines (WCAG). But don’t take our word for it — check... -
2022 and 2023: Reflections and Looking Forward
TypeBlog Post...commitment to top notch quality service and support. Alongside our material offerings, in 2022 we also rolled...website saw 2.2 million visitors from 231 countries. Guides and protocols were our most popular content, while... -
A Conference By Postdocs For Postdocs: Future of Research
TypeBlog Post...opportunities for career development and networking. Aside from the importance of the topics to be discussed...academic administrators who are finding ways to provide new types of career preparedness training for their... -
Keeping Up With CRISPR/Cas9
TypeBlog Post...quoted as saying when the news first broke. "Considering that many human diseases are caused by genetic... and mainstream the news on CRISPR has gotten, consider these headlines in The New York Times and Forbes... -
Measuring Kinase Activity at the Single-Cell Level with Kinase Translocation Reporters (KTRs)
TypeBlog Post...design and difficult to multiplex. The Covert Lab provides an alternative tool with their Kinase Translocation...nuclear translocation rates are another variable to consider when designing KTR experiments. Third, KTRs are... -
Developing Transferable Skills During Science Training
TypeBlog Post... who started the BALSA group, a nonprofit that provides short-term consulting services. Initiative and...?" Form Your Own Peer Mentoring Group: A How-To Guide for Scientists Download our eBook: Mentoring for... -
The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years
TypeBlog Post... life sciences. In this post, we'll give you an inside look at the Top 10 plasmid technologies distributed...Plasmids Shipped! - By 2010, Addgene had hit our stride. We shipped our 100,000th plasmid. A variety of... -
What's New in CRISPR - December 2019
TypeBlog Post...this system was optimized for Burkholderia, they identified 67 genera of Proteobacteria that this toolkit...sheet Resources on Addgene.org Read our CRISPR guide Find CRISPR plasmids by function Find recently deposited... -
Six Spooky Science Stories and Halloween at Addgene
TypeBlog Post...wall of green algae (Soo et al., 2015). There, it divides into two cells. At the same time, it sucks out ...Spinochordodes tellinii‘s larvae makes its home inside grasshoppers and crickets. When it’s ready to transform... -
IDP and your PI: A Roadmap for Career Planning and Personal Development
TypeBlog Post...Science Careers’ myIDP tool. Using myIDP, you can identify suitable careers based on your current interests...anxiety of graduate and postdoc training. On the other side, the PI has an opportunity to check in with lab ... -
Tag Your Favorite Yeast Genes with Ease
TypeBlog Post... own unique protein fusions. This collection is ideal for tandem affinity purification of protein complexes... describe a modular collection of plasmids for a wide variety of genome modifications in S. Pombe, including... -
New Norepinephrine Indicators: nLightG and nLightR
TypeBlog Post...antagonist. This is important because alpha2-AR can provide inhibitory feedback in response to norepinephrine...Optogenetics Plasmids Visit the Fluorescent Protein Guide Pages ... -
pSiM24: Simplifying Plant Genetic Engineering
TypeBlog Post...respectively, making it ideal for large gene transfer. The M24 promoter provides constitutive, high-level... -
6 Steps to Submitting a Resume That Gets Seen
TypeBlog Post...dramatically increase the chances of being seriously considered for an open position. Check out Joanne's Reddit...network your way to a hiring manager. Learn to confidently describe your strengths in your resume and don't... -
Future of Research Conference - Remarkable Opening Session
TypeBlog Post...Boston University last week. This fantastic city-wide effort was led by two amazing postdocs, Kristin ...about academia and alternate training needs to be provided to take advantage of all the possible science ... -
London Calling - Addgene’s New Europe Office in London
TypeBlog Post...way to share their plasmids. Now I am on the other side of the force and as an Addgenie I will do all my...scientific discovery or invention, and sharing those ideas with others. This love of interacting with people... -
An Addgene Summer Internship
TypeBlog Post...back from other summer programs. Amy took into consideration just how busy I was and ensured that my decision...concentration of a reagent during our HiFi assembly but decided to go ahead anyway. We were running on a time crunch... -
Hot Plasmids - October 2022
TypeBlog Post...from the labs of Mingshan Xue and John Spudich identifies a novel class of light-gated potassium channelrhodopsins...KCRs), from a fungus-like protist Hyphochytrium catenoides (Govorunova et al. 2022). The authors show that... -
SunTag and Fluorescent Imaging
TypeBlog Post...SunTag, they attempted single-molecule imaging deep inside the cell, in the nucleus and cytoplasm. Again, ...Protein Empy Backbones Visit Our Fluorescent Protein Guide Page Find Luciferase Plasmids ... -
What's the Best Way to Elute and Store Your Plasmid DNA?
TypeBlog Post...nuclease free water is a great option because it provides a great versatility on how to use your plasmid...Storing DNA: temperature and longevity You should consider a couple of more things when you elute your DNA... -
Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation
TypeBlog Post...the SPARC system, attP sites are located on either side of a stop cassette, upstream of an effector gene...Cell-type-specific and projection-specific brain-wide reconstruction of single neurons. Nature Methods... -
Viral Vectors 101: Parts of the AAV Packaging Plasmid
TypeBlog Post...occurring AAV serotypes that have thus far been identified: AAV1-11. Nine regions of variability in the ...type tropism. Introducing changes and/or short peptides sequences into these variable loops is one method... -
Sensing Neuronal Dopamine
TypeBlog Post...well-known reward chemical and neurotransmitter. But aside from DA’s famous role as the “happy hormone,” it...Addgene's viral service Find viral protocols and videos Find biosensor AAVs for your research ... -
Management for Scientists: What Makes a Good Manager Anyway?
TypeBlog Post...Intelligence or to hone delegation skills, for example. So aside from a focus on removing obstacles, what makes a... (this actually varies, a manager does best to identify the work style of each team member) Need challenging... -
New Viral Vectors - Winter 2025
TypeBlog Post...Bundling up for winter? The weather outside might be frightful, but browsing the 49 new preps available... vectors? Browse our in-stock viral vectors or consider our AAV Packaged on Request service, which offers... -
Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC
TypeBlog Post...primary antibodies can be expensive and may not provide enough signal to visualize low-abundance targets...your primary and secondary antibodies as normal. Ideally, your secondary will only bind to your primary ... -
What the HEK?
TypeBlog Post...aborted fetus. HEKs have an unusual ploidy and are considered hypotriploid: most chromosomes are represented...293 is known for reproducible results. It’s also widely available, which can make for a seamless transition... -
Antibodies 101: Epitope Availability
TypeBlog Post...available for binding change as well. If this change hides the epitope that the antibodies used to recognize...bind to the epitopes that are available on the outside of the cell. The same protein may have different... -
A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light
TypeBlog Post... Di Ventura on Twitter, you might have seen the video of her singing about the paper recently published... transcription from the PBAD promoter. “AraC is widely used in synthetic biology, biotechnology, and almost... -
CRISPR Challenges: Standardization and Homology Directed Repair
TypeBlog Post...CRISPOR, a tool to help you design, pick, and close guides for Cas9 and Cpf1. This tool supports over 250 ...Mechanisms of precise genome editing using oligonucleotide donors". Kan, Yinan, Brian Ruis, Taylor Takasugi...