We narrowed to 643 results for: ATO

1. Why and how to start studying chromatin (with Addgene's help)

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   ...Chromosome Organization. These aren’t the only investigators in the chromatin field who have deposited with...

2. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

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   ..., the stem-loop structure of transcriptional terminators. Another potential issue is sequence similarity...

3. Viral Vectors 101: Gamma-Retroviral Packaging Systems

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   ...Woodchuck hepatitis virus post‐transcriptional regulatory element; sequence that stimulates the expression...

4. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

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   ...microRNAs (miRNAs) are recognized as important regulators of post-transcriptional gene expression across...

5. Building and Validating Recombinant Antibodies: A Case Study

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   ...secondary antibodies are commonly used in research laboratories to detect the different subclasses, but there...

6. Sharing is Caring: But How to Distribute Open Hardware?

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   ... in science brings stronger networking across laboratory and disciplinary boundaries and can allow for...

7. Plasmids 101: Screens vs. Selections

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   ...selected growth conditions. For screens, there is no obligatory growth advantage – that is, you are not specifically...

8. From Lab Bench to Leadership: Transitioning into Management as a PhD

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   ...and leveraging tools like the Myers-Briggs Type Indicator or DISC assessments can help you navigate team...

9. Behind the scenes: Addgene’s new search engine and more

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   ...of other websites, we built our new search engine atop Elasticsearch, a powerful open-source search engine...

10. Antibodies 101: Monoclonal Antibodies

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    ...the antigen-binding sites of the antibody - the paratopes - may change and impact the specificity and avidity...

11. A Practical Approach to Choosing the B(right)est Fluorescent Protein

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    ...been used in yeast by Lee et al (2013) and in nematodes by El Mouridi et al (2017) and Heppert et al (...

12. Multiplex Genome Editing with CRISPR-Cpf1

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    ...multiplexing vectors are larger because they require regulatory sequences to allow for expression of multiple...

13. Top Requested Lentivirus and AAV of 2016

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    ..., Koda S, Ye C, Rogan SC, Adams AC, Cusher DS, Maratos-Flier E, Roth BL, Lowell BB. Rapid, reversible ...

14. How to Make Friends and Meet People at a Scientific Conference

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    ...You never know when you will meet a potential collaborator Some of these people you meet will review your...

15. Dos & Don'ts When Publishing a Scientific Manuscript

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    ...communities. The ARRIVE guidelines for the reporting of laboratory animals during in vivo research and the CARE ...

16. Bacterial Expression Systems

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    ...Two-Hybrid System Various Plasmids placO with lambda-C1 operator Beta-galactosidase Ann Hochschild Bacterial two-hybrid...

17. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

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    ...of IPTG. Because RepA is a plasmid replication initiator protein, they could alter copy number of the plasmid...

18. RaPID Detection of RNA-protein Interactions

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    ...one to study RNAs expressed from their native regulatory elements. Controls for RaPID experiments RaPID...

19. dTAG - You're it!

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    ...dTAG-47 Weintraub et al. YY1 Is a Structural Regulator of Enhancer-Promoter Loops. Cell. December 14 ...

20. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

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    ...the Hell lab designed their super-resolution pH indicators (SRpHi) biosensors to meet all of them. These...