...proteins included in different CGBE tools allow the researcher to direct the repair of the abasic site. But ... originally being developed for A → G edits, researchers tried inhibiting MPG/AAG to improve editing efficiency...transversion editors were developed, though, researchers at HuidaGene Therapeutics guessed that fusing...existing CGBEs. This distinct approach means researchers can now select the best tool for their application...

...adaptation for use in mammalian cells in 2012-2013. Researchers are now using this system in ever more creative...Hopefully this post will help get your in vivo research up and running as soon as possible! Selecting ...it’s time to use them in mouse embryos. When researchers first started using CRISPR/Cas9 for genome editing...RNA-guided endonucleases and nickases." Genome research 24.1 (2014): 132-141. PubMed PMID: 24253446. PubMed...

... A recent survey of PhDs found that many researchers feel that they lack formal training in a variety...out to fill this gap by both highlighting that researchers do learn MANY transferable skills while working... variety of ways to manage my time. As a busy researcher, you’ve probably already started coming up with...hope you’ll find this post useful. After a bit of research and thinking about my own processes, I’ve broken...

... contributed by Jacob Lazarus, a postdoctoral researcher at Harvard. There’s an astounding number of ways...attending physician. He performs post-doctoral research in the lab of Matthew Waldor at Harvard. Now that...sliding at upstream standby sites." Nucleic acids research42.4 (2013): 2646-2659. PubMed PMID: 24234441. PubMed... unmodified Enterobacteriaceae." Nucleic acids research 43.13 (2015): e83-e83. PubMed PMID: 25800749. ...

...products that resulted from their research, “aha” moments, and future research directions. Don’t worry if your...this file over the years and a talk about your research or work will write itself when the time comes....ask about how many people use plasmids in their research and then see how many of them have requested from...

...through the use of invasive methods, limiting researchers to accessible, spatially constrained regions.... it’s always helpful to start with a literature search to see what others have done, you’ll need to experimentally...precursor. Considering systemic capsids for your research? While systemic capsids mostly function like your...depicting the phylogenetic relationship of common research species. Among these species, Old World Monkeys...to the nonhuman primate nervous system. Current Research in Neurobiology, 4, 100086. https://doi.org/10.1016... central nervous system. Nature Cardiovascular Research, 1(4), Article 4. https://doi.org/10.1038/s44161...

...increasing affordability and accessibility, more researchers have been utilizing full plasmid sequencing services...sequencing that Addgene uses.) However, when some researchers received the sequencing results for their plasmids...numbers (Williams, Carnes, and Hodgson, 2009). More research is ongoing to determine plasmid features that ...monomeric plasmid compared to DH5α. However, more research is needed to determine which recA- strains are...

...the complexity of the system, researchers have used split GAL4 architecture or combined the system with ... ON state, and how you might use these in your research. Today, we’ll look at repressible promoters, which...bind the promoter and repress pADH1 activity. Researchers have engineered pADH1 variants that are less ...

... lab website, publication records, LinkedIn, ResearchGate or Twitter. Be ready for the interview with ...with the rise of movements such as the Future of Research Symposium and #MeTooSTEM, academic bullies are...first place. This is another reason to do your research and choose wisely. From the Tenure She Wrote blog... to consider less expensive, portable areas of research (transgenic mice—out, zebra fish—in) Team attitude...

...effectiveness and interpretation of RNAi. Fortunately, researchers studying plants and other organisms lacking this...concatenated. hpRNA has been extensively used in plant research and many different constructs are available to...and is a popular RNAi trigger in the mammalian research community. Fortunately, many tools for shRNA in...fluorescence readout of RNAi activity. As a plant researcher, the traditional hpRNA and visual screening method...

...how and where you pursue it. It can manifest via research and data gathering, regulatory efforts, policy...fellowships have a strong focus on early career researchers who have decided to transition out of academia... host “Hill Days” where they train and bring researchers to Congress to advocate for increased science...incentivizing the real-world impacts of health services research. She is particularly interested in driving scientific...

...processes that are crucial in the world of biological research, medical therapy, and even drug development (Grimm...stable, and versatile imaging tools in biological research. This does make finding the right dye for your...complicated. We hope this post helps you in your search for the perfect way to light up your science. Simon...Methods (Vol. 19, Issue 2, pp. 149–158). Nature Research. https://doi.org/10.1038/s41592-021-01338-6 Grimm...

... novel aminoacyl tRNA synthetase/tRNA pair, a researcher can lure the ribosome into inserting a fluorophore-tagged...beyond the FRET range of the donor (i.e. >100 Å). Researchers have developed an extensive array of chemical...currently an Assistant Professor in the Anesthesia Research department at Brigham and Women’s Hospital in ... of proteins in living cells." Cell and tissue research 360.1 (2015): 179-194. PubMed PMID: 25743694. ...

...reagents and data, we’re also publishing research. Addgene’s research team also investigates new strategies...vector jokes and comics. Other new resources for researchers using viral vectors In addition to “going viral...

... an important animal model for research, particularly genetics research, for decades. Its short life cycle...filmed at Addgene:     If you're working in fly research, I hope you find this new technology as helpful...

...publication where you described them. Search for your article as you would search for it in PubMed and choose the... share plasmids. To achieve this goal, we will archive any plasmids you've deposited with us and distribute... your plasmids are online and available to the research community. This article was originally written...

...technology is so useful it forever enhances biological research and discovery. CRISPR is a great example (the ...infect many different types of cells. This means researchers who use VSV-G can deliver their genes of interest...autophagy, CRISPR, and so much more (find the full, searchable list here). Tips for using pMD2.G At Addgene,...

...kept Addgene at the forefront of life sciences research for the past decade. We’ve also learned along ...Summaries of the latest technologies and breakthrough research. Insights about how to navigate through all ...graduate student, post-doctoral fellow, lab manager, research technician, or Principal Investigator. How-to...

...plasmids? Do you spend a lot of time in PubMed searching for research articles? Well then, my scientist friend...Filters” page, select the category “LinkOut” and search for “Addgene”. Then check the “Link Icon” box next...

...AAV9 Biosensors Patriarchi New viral service pAAV_hSyn1_nLightR AAV9 Biosensors Patriarchi New viral service...we've made to our viral vectors repository since March.  Addgene is now offering an AAV2 CAG-driven tTA...