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  1. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    ... common event. What are exosome AAVs? In 2012, Casey Maguire and colleagues from Massachusetts General...28082074. PubMed Central PMCID: PMC5368844.  Maguire, Casey A., et al. "Microvesicle-associated AAV vector as...

  2. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    ...computer modeling and predictive algorithms to forecast the drug epitopes likely to be presented by T ...between 2D assays and in vivo animal models. In these cases, they are designed to mimic the human lymphatic ...

  3. Cultivating Community Science at BosLab

    Type
    Blog Post
    ...we all shared a passion for DIY Biology and, on occasion, would meet in the same space where BosLab exists...journal club discussion on Kevin Esvelt’s CRISPR/Cas9 gene drive paper was very popular. People kept asking...

  4. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    ... start of the CRISPR craze, but he says, “In the case of CRISPR, the hype was completely justified… it...the audio engineer and producer for the Addgene Podcast.       Resources at Addgene Find CRISPR resources...

  5. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    ...of having all the pages in a Curricula Vitae to showcase your publications, academic performances, and ...related to the job you are applying for, in which case talk about your research, as an understanding of...

  6. Finding and Joining Your Dream Lab

    Type
    Blog Post
    ...should be interesting; however, this should (in most cases) not be the driving factor. The following questions...head for a career in pharma or biotech, this is one case where the topic of your research can make your transition...

  7. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    ...up positive traits (i.e. the plasmid contains a cassette that will make cells resistant to a toxin), whereas... a negative trait (i.e. the plasmid contains a cassette that will make cells sensitive to a toxin). In...

  9. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    ...following exposure to a brief heat shock. In the case of Hsp70, the heat shock releases heat shock factor...depositors have developed heat shock-inducible Cre and Cas9 for easy genome engineering in species like C. elegans...

  10. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    ...photons or odorants, to elicit downstream signaling cascades.  So let’s break down the name "G-protein coupled...then induce or inhibit intracellular signaling cascades as a response to the extracellular stimuli. Ligand...

  11. Antibodies 101: ChIP

    Type
    Blog Post
    ...validate your antibody on crosslinked proteins. In the case of histone modifying proteins, which bind quite ...abundance transcription factor or cofactor. In such cases, the gentler enzymatic digestion can preserve more...

  12. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    ...during in vivo research and the CARE guidelines for case reports are two sets of guidelines frequently used...manuscript is accepted for publication and is, in most cases, blind (meaning you don’t know who the reviewers...

  13. All in a Twist: dsRNA

    Type
    Blog Post
    ...given threshold within the cell, as is often the case upon viral infection. Endogenously produced dsRNA...the Addgene blog CRISPR 101: RNA Editing with Cas13  RNA Interference in Plant Biology  ...

  14. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    ...intensity remains. The first issue is that, in several cases, the fluorescence intensity decay of a fluorophore... the excitation power (Grünwald et al, 2008) occasionally and certainly after a set-up has been changed...

  16. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    ...primary-conjugated) or indirect (secondary) methods. In those cases, remember that a secondary should be used when signal...you still have a number of options. If this is the case, you can narrow them down in a variety of ways. ...

  17. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    ...oligonucleotides specifying a gRNA target sequence into a Cas9-containing plasmid such as pX330. This cloning strategy...are using the Golden Gate method to create CRISPR/Cas9 constructs, assemble standard plasmids parts in ...

  19. A Look at Addgene's QC Process

    Type
    Blog Post
    ... process, following sequencing and assembly. In cases where the QC team suspects an assembly issue, they...and be made available for distribution. In these cases, the QC team leaves a note on the plasmid page to...

  20. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    ... Let’s get started! Before you begin In the best case, you know ahead of time that you want to probe your... to lose by trying to strip and reprobe. In this case, we recommend using mild stripping solution (see...
Showing: 461 - 480 of 711 results