Visualizing Protein Turnover In Situ
Type
Blog Post
...Use FRET to study protein-protein interactions
Deposit plasmids with Addgene!
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We narrowed to 660 results for: EPO
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Choosing the B(right)est Fluorescent Protein: Aggregation Tendency
TypeBlog Post...research. But be aware that the fluorescent proteins reported in publications are only characterized under a... -
CRISPRainbow and Genome Visualization
TypeBlog Post...technique was developed in the lab of Addgene depositor Stanley Qi and used in Chen et al. to track telomere... -
Gendered Innovations: Why Does Sex of the Cell Matter?
TypeBlog Post...of Research on Women’s Health, emphasizes that reporting the sex of cells and tissues—when that information... -
Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening
TypeBlog Post... for West-Nile-virus-induced cell death." Cell reports 12.4 (2015): 673-683. PubMed PMID: 26190106. PubMed... -
Career Coaching for Scientists: Why and Where Do I Find One?
TypeBlog Post...Thesis (who has also blogged for us) offers a free report on her website called “Secrets to Success in Graduate... -
CRISPR Plasmids - Tagging
TypeCollection...The Eric Mendenhall and Richard Myers labs have deposited plasmids for a CRISPR-based system to add a tag...homology arms and selection marker. The first deposited plasmids in this CRISPR-Cas tagging system were... Tagging System Recently, Yannick Doyon's lab deposited a plasmid which introduces an N- or C- terminal...PITCh Tagging System The Takashi Yamamoto lab has deposited the PITCh system plasmids for CRISPR-based knock-in...microhomologous sequences and gRNA target sites. The first deposited PITCh plasmids were tested by fusing EGFP-2A-PuroR...with the following article: Natsume, et al. Cell Reports 2016 Förstemann Drosophila Cell Tagging System ... -
Design Tips for Prime Editing
TypeBlog Post...that meant rereading many of the original papers reporting various prime editing tools. These papers are ... -
Antibodies 101: Stripping and Reprobing Western Blots
TypeBlog Post...your western blot several times — some researchers report up to ten cycles! Just remember that a bit of sample... -
High Complexity Golden Gate Assembly with NEB’s DAD
TypeBlog Post... to start is the 24-piece lac operon assembly deposited by NEB – and don’t forget to ask DAD for some ... -
Antibodies 101: Choosing the Right Isotype
TypeBlog Post... reactive ones. And make sure to check for any reported cross-reactivity when planning your experiment... -
Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors
TypeBlog Post...ways how scientists have used Addgene’s plasmid repository and viral service for their behavioral studies... -
Mouse Modeling, Part 1: Genetically Engineered Mice
TypeBlog Post...vectors used to create knockout mice include a reporter gene, which replaces the targeted gene and provides... -
Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation
TypeBlog Post...sigh. The problem is that many of these studies report correlations without clearly testing whether a ... -
A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells
TypeBlog Post...selection of guideRNAs. Of particular note, ABE8s reported in this work did not cause genome-wide, guide-... -
Plasmids 101: Modular Cloning
TypeBlog Post...As a plasmid repository, we are no strangers to cloning. If you’re reading this, then chances are you... -
5 Tips for Troubleshooting Viral Transductions
TypeBlog Post... and the type of virus, there have been varied reports of titer losses from 5% to 50% per freeze thaw ... -
Plasmids 101: Terminators and PolyA signals
TypeBlog Post...characterized a set of prokaryotic terminators and deposited several with Addgene (1). Prokaryotic polyadenylation... -
The Materials Science of Optogenetics Experiments
TypeBlog Post...wirelessly-controlled optogenetic implants have been reported by several groups [4, 5, 6]. These have the obvious... -
How-to: 5 Steps to a Great Panel Discussion
TypeBlog Post...published on LinkedIn. Follow Addgene on LinkedIn for repository news and updates. Panel discussions are becoming...