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We narrowed to 1,145 results for: Gene

Showing: 461 - 480 of 1145 results

  1. The SGC Stockholm Collection

    Type
    Collection
    ...Genomics Consortium Plasmids - Stockholm Plasmid Gene/Insert Vector Type Mutations Tags Industry PI...

  2. New Educational Resource: The Addgene Videos Page

    Type
    Blog Post
    .... Along with our role as a plasmid repository, Addgene aims to be a vital educational resource for scientists...mind, we are very excited to debut our brand new Addgene Videos page! Videos are a powerful tool for communicating...or to answer any questions you might have about Addgene. The page is divided into three main sections: ... real Addgenies who have a variety of roles at Addgene.  You’ll hear all about their career paths, future...feature screen-capture footage to help you navigate Addgene’s vast online resources. Our most popular How-To...stage of the selection and checkout process on Addgene’s website: We’ll be continuously updating this...comments section below! Additional Resources on the Addgene Blog 5 Science Rap Videos and Their Creator: Tom...

  3. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    ...their mission. Addgene is fortunate in that we are able to fulfill our mission by generating revenue through... is a bit misleading. Nonprofits can generate revenue (as Addgene does through materials distribution)... at Addgene If you’re ever curious what specifically drives the decision making process at Addgene, just...in the community Addgene fosters a culture of respect, collaboration, and fun Addgene believes there is... on the Addgene Blog Learn about Our Collaboration with Fellow Nonprofit Seeding Labs Addgene Supports...to Our Interview with Addgene Board Member, Connie Cepko Resources on Addgene.org Learn More About Our...Our Mission Meet the Addgene Leadership Team Get the Latest News from Addgene ...

  4. Addgene is Expanding Our Viral Vector Service!

    Type
    Blog Post
    ...Subscribe to the Addgene newsletter for updates on all things Addgene; or subscribe to the Addgene blog for updates...request an AAV composed of an AAV plasmid in the Addgene repository and their choice of five different serotypes...comes to ordering high-quality materials from the Addgene repository. Read on to learn more.  What will be... be available?  Over 3000 AAV plasmids in the Addgene repository will be available for Packaged on Request...request. Request details can be accessed within your Addgene account. Preps will be available for request in...Once submitted, the request will be reviewed by Addgene for legal approvals and technical compatibility...Cart” button will appear with the request in your Addgene account.  Orders do not need to be placed immediately...

  5. How Do I Place an Addgene Order Using a PO Number?

    Type
    Blog Post
    ... is easy. Ordering genes on plasmids online is a little trickier -- but at Addgene, we try to make the...here at Addgene. If you’ve called our customer service line or sent an email to help@addgene.org, you’ve...seller. However, Addgene is a bit different from other vendors. As a nonprofit, Addgene does not own the... bigger question: How does a customer place an Addgene order using a PO number? Why do I have to order...distribute it on behalf of depositing institutions. Addgene cannot fulfill orders based on PO documents that...information that is entered at checkout is used to generate the legal agreement (MTA), which streamlines the... with the recipient organization can create an Addgene account and place an order: a purchasing agent,...

  6. Meet Aavery, Blugene's New Virus Friend!

    Type
    Blog Post
    ...Welcome to Addgene, Aavery!       Original post: In December, we introduced you to Blugene’s new virus...March 6, 2020. After months of input and polling, Blugene's new virus friend finally has a name! We thank ...we’re excited to see your thoughts. Our mascot Blugene can’t wait either. Acknowledgements We would like...

  7. Help Us Name Blugene’s New Virus Friend

    Type
    Blog Post
    ...on the Addgene blog Learn more about Blugene's make-over Get a how-to guide on the new Addgene search... this form or the hashtag #BlugeneVirusFriend on Twitter. As with Blugene’s name, we’d like to give their...receive some newly designed Addgene swag. Have fun! Suggest a name for Blugene's new virus friend here Additional...eight years now has a new friend to join them. Blugene would like you to meet...  Hmmm, they need a name... we need your help in finding a good name for Blugene’s virus friend.  During the month of January,...

  8. Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight

    Type
    Blog Post
    ...throughput studies of optogenetics.  Common opsins used in zebrafish Optogenetics functions through opsins....  Additional resources on the Addgene blog Find all our optogenetics blog posts Read blog posts about... viral vectors Subscribe to the Addgene blog Resources on Addgene.org Learn about our ready-to-use viral...Optogenetics, the use of light sensitive proteins (opsins) to manipulate cell activity, enables researchers...escape reflex. These traits, coupled with short generation times and high fecundity, make zebrafish ideal...are light sensitive proteins. Opsins used in optogenetic studies are not native to vertebrates and are...swimming  Researchers at the Isacoff lab applied optogenetics in zebrafish larvae to identify new neurons ...

  9. Addgene Protocols Go Viral: AAV Purification and Titration

    Type
    Blog Post
    ...content from Addgene, subscribe to Addgene’s YouTube Channel. Additional resources on the Addgene blog Learn...about Addgene's Lab Tips video series Resources on Addgene.org Find lab protocols on the Addgene website...Since rolling out our viral service in 2016, Addgene has shipped thousands of AAV and lentiviral preps... purity of all AAV viral lots. Aligning with Addgene’s mission to “accelerate research and discovery ...improve your experiment results! Subscribe to the Addgene YouTube page! AAV purification by iodixanol gradient...calculations on how to prepare a plasmid stock used to generate a standard curve, visit the AAV Titration by qPCR...website Watch Addgene's video collection ...

  10. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ... time or location can be critical when, say, the gene is essential during development and a knockout is...the Addgene blog Viral Vectors 101: Optogenetics Additional resources on Addgene.org Optogenetics Guide... The Chow Lab has also generated a variety of membrane-recruitment optogenetic tools for manipulating ...a full toolbox and workflow for generating new Rho GTPase optogenetic systems (Berlew et al., 2022). Stopping...LOV, CRY2-CIB, and other optogenetic modules? Or want to find more optogenetic tools in our collection?...collection? Check out our Optogenetics Guide or browse our collections of optogenetics plasmids – and keep an eye...Optogenetics gives you the power to control cells or organisms with the flip of a switch. You may be ...

  11. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    ...a second plasmid will be needed to introduce the gene of interest containing the chosen codon that will...limiting. It may be time, then, to look towards genetic code expansion (GCE): techniques in which non-canonical...essentially those needed to make site-directed mutagenesis, many researchers new to GCE aren’t able to successfully...added phosphoserine. A community resource Like Addgene, GCE4All is a community resource which depends ...researchers. One way of doing that is by using Addgene’s Blue Flame award to identify highly requested ...the plasmids and bacterial strains (available at Addgene for GCE work. Plus, keep an eye on our blog – we...., Griffith, M. C., & Schultz, P. G. (1989). A General Method for Site-specific Incorporation of Unnatural...

  12. Addgene’s First AAV Data Hub Challenge

    Type
    Blog Post
    ...We're going virtual for Addgene’s first AAV Data Hub Challenge, now taking place Dec. 6, 2022! Share ...Anyone can submit data from their CLOVER lab or Addgene AAV experiments to the Data Hub. Submit your data...travel award. All participants will receive an Addgene t-shirt.  Our judges, Drs. Connie Cepko, Ian Wickersham...

  13. Addgene's Top Blog Posts from 2020

    Type
    Blog Post
    ...to highlight some of the top articles from the Addgene blog this year. Spoiler alert: many of the top ...subset of the fluorescent protein family and they generate color! They've been adapted for many molecular...SARS-CoV-2, including many with plasmids deposited at Addgene! 2. DNA Purification Without a Kit by Beth Kenkel...

  14. Suggest A Plasmid You'd Like to Find at Addgene

    Type
    Blog Post
    ...experiment!” but were unable to find the plasmid at Addgene? Well, we’re here to help with our Suggest a Plasmid...Campaign. How to suggest a plasmid The scientists at Addgene work hard to seek out plasmids to add to our collections... potential depositors. 2. Tweet at us! Tweet @Addgene with #suggestaplasmid, the name of the plasmid ...

  15. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    ...and genetics. It can be used to characterize the function of certain regions in a promoter or gene, as...experiments. When REPLACR-mutagenesis goes head-to-head with Gibson assembly and GeneArt cloning, the results...REPLACR-mutagenesis could save busy scientists a lot of time. Let us know how REPLACR-mutagenesis works ...your hands!     REPLACR-Mutagenesis Megaprimer PCR Gibson Assembly GeneArt Cloning Types of Mutations...Comparison of REPLACR-mutagenesis with other common methods for site directed mutagenesis   References 1. ...al. (2016). “REPLACR-mutagenesis: a one-step method for site-directed mutagenesis by recombineering.” ...Site-directed mutagenesis (SDM) is one of the key tools researchers use to prove causation in molecular...

  16. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    ... Benjie Chen co-founded Addgene in 2004. They worked tirelessly to get Addgene from a concept to an up... culture here at Addgene has remained paramount. Candidates who interview at Addgene frequently ask the...working at Addgene?” Most Addgenies reply with an enthusiastic, “Our company culture! Addgene is the best...ideas and company. The Addgene summer picnic This past weekend was the annual Addgene Summer Picnic - a time... it was another successful event! Addgene company culture At Addgene we organically developed the philosophy... happened naturally when Addgene was a family affair back in 2004. As Addgene has grown, it has taken ...Our story today starts with a bit of Addgene history... As you may be aware, Melina Fan, her brother ...

  17. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    ... be done with RNAi in terms of determining what genes are involved in biological process X, you can do...help guide and promote Addgene in its mission to make research easier. Addgene is excited to announce ...use in genetic screens and has contributed much of his knowledge to the CRISPR resources at Addgene. His... is what got him involved with Addgene - he says he’s “known of Addgene basically since the beginning ...ethic that Addgene has behind it is something that very much resonates with me.” About Addgene’s role in...Doench says, Addgene is “a central resource for what is worth having. The work that [Addgene’s] outreach ...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.       Resources...

  18. Addgene’s Top 10 Blog Posts from 2014

    Type
    Blog Post
    ...Joanne Kamens Plasmids 101: Antibiotic Resistance Genes By Marcy Patrick Plasmids 101: Yeast Vectors ...community, so email us at blog@addgene.org if you are interested. Addgene's Most Popular Posts of 2014 ...year in science – as seen through the lens of Addgene’s blog and plasmid repository. Our blog is just ... Competent Cells” has been extremely popular, generating many additional questions. We’re happy to see...

  19. Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering

    Type
    Blog Post
    ...of the optogenetic tools that are still missing. Optogenetic tools for neuroengineering Addgene: For those...brain through optogenetics research Addgene: What are some of the big problems that optogenetic tools can ...science.' Looking for Optogenetics Plasmids? Plasmids for Optogenetics Research (Addgene) Plasmids from Ed...populations of neurons at once. Addgene spoke to Boyden about this latest optogenetics advance, the big questions...brief history of optogenetics? How long has this idea been around? Boyden: Optogenetics is a toolset that...two-color pair. We'll be sending it to Addgene in a matter of days. Addgene: Can you tell us a little more about..., or other conditions. Addgene: You've contributed various plasmids to Addgene over the years, many of...

  20. Quick, Versatile Plant Transgenesis with GreenGate Plasmids

    Type
    Blog Post
    ...promoters, N-terminal tags, coding sequences of the gene of interest, C-terminal tags, plant terminators ...The complete GreenGate toolkit, now available at Addgene, also gives the option to stack several expression...rounds, dramatically reducing the time required to generate multi-construct transgenic plants. In the most...motivations for making GreenGate available through Addgene,” Lohmann said. “We strongly believe the free sharing...Versatile and Efficient Cloning System for Plant Transgenesis." PLoS ONE. 8(12): e83043 (20 December 2013)...
Showing: 461 - 480 of 1145 results