We narrowed to 855 results for: Met;

...combination of these methods provides unique opportunities to develop computational methods to better understand...UAS system for Caenorhabditis elegans." Nature methods 14.2 (2017): 145. PubMed PMID: 27992408. PubMed...

... this blog post that highlights five different methods to use CRISPR to detect SARS-CoV-2. Other protocols...Andrea Pauli and Julius Brennecke. They developed a method that uses loop-mediated isothermal amplification... after reverse transcription (RT-LAMP) and a colorimetric readout that can be completed in just 45 minutes...

...minute! Fun fact! Electroporation is one popular method scientists use to promote transformation, but it...offer resistance to toxic substances like heavy metals or pesticides, enabling their hosts to survive ...inducible expression systems that allow tuning of metabolic pathways. Fertility (F) plasmids: microbial sharing...

...relies on Gibson and other non-Golden Gate cloning methods. MoClo’s organization schema MoClo can be broken...which a precursor molecule is made, modified (sometimes many times), and isolated afterwards. To engineer...imagine, there is an array of distinct goals within metabolic engineering, synthetic product production, and...

...or poor cellular entry with other transfection methods. The Junwei Shi Lab addressed this problem by optimizing...-relevant lines such as CAR-T cells. Plus, this method is fast! These editing frequencies can be achieved...fusion and membrane-targeting applications. Nature Methods, 1–9. https://doi.org/10.1038/s41592-023-02085-...

...controls, safety systems that are built into how something functions, were designed for this purpose. One...understand what safety precautions to take, and if something does happen, ensure you can receive appropriate... before you start an experiment, just in case something does happen.   Finally, the best way to be safe...

... week): The AdEasyTM system is the most popular method for creating adenoviral vector constructs. It consists...required if rAdV is to be used in vivo. The standard method for purification of rAdV uses cesium chloride (...looking at absorbance at 260nm using UV/Vis spectrophotometry. An absorbance of at 260nm corresponds to...

...concentrating a viral stock. Different titering methods can reveal differences in physical versus functional...the result of successful viral transduction. This method may not work if the virus does not have the proper... tropism to infect the packaging cells. Another method of ensuring viral production is to perform PCR ...

...many cases fluorescent proteins have usurped old methods or complemented them in ways that make them much... now become hallmarks of ideal FP timers: 1. Ratiometric determination of expression time The ratio of...FP-timer protein or protein fusion. This one protein method simplifies cloning and makes your experimental ...

...after a few washes, binding is detected using a colorimetric reaction. After the ELISA step, the narrowed-down...remarkable 12 working days. While the lab used this method for COVID-19 research, this protocol holds promise...System for Characterization of Enzyme Variants. In: Methods in Molecular Biology. Humana Press, pp 133–148....

...decision a little easier if an industry postdoc is something that you’re considering. Like anything, there ...can’t afford to spend too much time working on something extremely risky or exploratory. Getting a job ...specific web sites for postdoc listings, so the method of posting varies between companies. Like most ...

...secretion of lactic acid (a by-product of cellular metabolism that is toxic to the cells), and need to be split...3. Contamination, Ileana S., John P. S., Basic Methods, Human Stem Cell Manual (Second  Edition), 2012...Culture Cloning Mammalian Cells with the Agarose Method Plasmids 101: Mammalian Vectors Resources on Addgene.org...

...laboratory populations with that sequence2. Both methods are easy to implement and have been tested in yeast5...can't be performed with two or more confinement methods. Since molecular, ecological, and reproductive .... M. & Bier, E. The mutagenic chain reaction: A method for converting heterozygous to homozygous mutations...

...interest, scientists have instead developed novel methods to co-express your gene along with a reporter, ...the genes expressed from the same cassette is sometimes advantageous, particularly when only a portion...use the 2A peptides instead of an IRES when stoichiometrically equivalent levels of expression are required...

...animal sacrifice. Could there be an alternative method for immunodetection? Enter the Görlich lab and ...and extracted with common protein purification methods. Read on to learn more about nanobodies and how...isolating HCab antibodies from its serum, and meticulously screening, optimizing, and characterizing several...

...Molecular cloning requires some method of screening colonies for the presence of an insert. Traditionally...mini-prep the positive ones. Regardless of which method you choose, make sure to use the appropriate antibiotic...amplify a product, then you know there could be something wrong with the PCR setup and/or the primer design...

...and its host response. Nat Methods 13:868–874 . https://doi.org/10.1038/nmeth.3993  Cong L, Ran FA, Cox...next-generation sequencing). Using this sensitive method, they found that SaCas9 did not display higher ...

...Egr3, and Arc. Thus, CRISPRa represents a powerful method to dissect the individual and shared contributions...surface protein for which there are good flow cytometric antibodies, downstream of the TUJ1 coding sequence...these cell types are used. The lack of good flow cytometric antibodies for many CNS-resident cell types (...

...higher than traditional homologous recombination methods). By using mCherry for selection of sgRNA plasmid...Hung, D. (2024). A dual-plasmid CRISPR/Cas9-based method for rapid and efficient genetic disruption in Mycobacterium...kDa) and are compatible with common microscopy methods. Plus, the luminescence wavelength (460 nm) is ...

...story. Viral vectors are, after all, a delivery method. I would be remiss to talk about them without looking...for Adeno-associated viral vector production. Neuromethods, 195, 77–101. https://doi.org/10.1007/978-1-...2019). Adeno-Associated Virus Technologies and Methods for Targeted Neuronal Manipulation. Frontiers in...