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We narrowed to 770 results for: Mos;

Showing: 461 - 480 of 770 results

  4. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    ...similar homology to the site of interest or create mosaic animals where not all cells in the blastocyst were...small molecule that regulates Cre expression. The most common drug used to induce the expression of Cre...

  5. A Look at Addgene's QC Process

    Type
    Blog Post
    ...prone to error — both human and biological. Even the most high-fidelity polymerases can make mistakes and ...and highly repetitive sequences and/or features. Most of these regions are identified during the QC process...

  6. Design Tips for Prime Editing

    Type
    Blog Post
    ...Primer binding sites with 40–60% G/C content are most likely to be successful, although sequences with...which helps users select pegRNA designs that are most likely to drive efficient editing. This is not the...

  8. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    .... As the depositing scientist, you will have the most involvement with Step 1, and, while data entry can...every so often and this ensures you are using our most up-to-date version! We hope the deposit spreadsheet...

  9. Hot Plasmids February 2024

    Type
    Blog Post
    ...in young macaques and vascular bias in mature marmosets. CAP-Mac also robustly expressed fluorescent reporters...(amino acid sequence DYKDDDDK) may be one of the most versatile. Whether you plan to purify or image a...

  10. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ...depending on what cell type it is expressed in. The most common target for chemogenetic manipulation are ...have been derived from muscarinic receptors. The most popular agonist for muscarinic receptor derived ...

  11. Engaging with science and society at pgEd

    Type
    Blog Post
    ...well as schools, community events, churches or mosques. On occasion, we also get interviewed for print...fields of research, what often concerns them the most about research is its implications for their lives...

  12. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ...://pubmed.ncbi.nlm.nih.gov/32675376/ mGold, the most photostable yellow fluorescent protein to date By... as bright to mVenus, the parent YFP, and is the most photostable YFP to date. As such, mGold is expected...

  13. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    ...some engineered terminators come close (>95%). For most purposes, however, any common terminator will suffice...Addgene (1). Prokaryotic polyadenylation Although mostly thought of as a eukaryotic-specific process, prokaryotes...

  14. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    ...the target cell’s genome. Physical titer The two most common assays for physical titer are direct p24 ...choice can also impact transgene expression. The most accurate method of lentiviral titration uses qPCR...

  16. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    ...DNA polymerase from the thermophilic bacterium, Thermos aquaticus (Taq). Taq polymerase is thus a heat ...presence, size, and relative quantity. End point PCR is most commonly used in molecular cloning, sequencing, ...

  20. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    ...One of the most powerful strategies to investigate a gene's function is to inactivate, or "knockout",...of the targeted gene will be removed from the chromosome and and replaced by the resistance gene.The gene...
Showing: 461 - 480 of 770 results