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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 22 results for: Mos;

Showing: 1 - 20 of 22 results

  1. CRISPR Library Amplification

    Type
    Protocol
    ...than four Maxipreps worth. Count colonies on the most dilute Petri dish. Total colony yield = count x ...in library. Example: At least 10 colonies on the most diluted plate for 1000x coverage of a library of... are present. If colonies are not present on the most dilute plate, count the second plate (ie. the 1:...tend to report lower values than Nanodrop, although most protocols have been designed with Nanodrop as the...

  2. Protocol - How to Ligate Plasmid DNA

    Type
    Protocol
    ...been generated by restriction enzyme digestion . Most restriction enzymes digest DNA asymmetrically across...size of each and their concentration. However, for most standard cloning (where the insert is smaller than... + Background due to vector re-circularization - most useful for phosphatase treated vector Insert or ...

  3. Using a Light Microscope Protocol

    Type
    Protocol
    ...so, for this protocol, we'll focus on one of the most common types that you're likely to encounter: the...properties of light to detect small objects. Two of the most important properties of a microscope are magnification... will see several pieces of information, but the most important (for now) is the magnification power, ...

  4. Protocol - Over-Agar Antibiotic Plating

    Type
    Protocol
    ...gently spread over the surface until the liquid is mostly absorbed (there is a very small visible volume ...gently spread over the surface until the liquid is mostly absorbed. The spreading of cells can be done in...

  5. Protocol - How to Purify DNA from an Agarose Gel

    Type
    Protocol
    ...following amendments: Notes: Gel purification is most efficient with lower % agarose gels, so you will...will want to isolate the DNA from the gel. This is most commonly done with a commercial gel purification...

  6. Protocol - How to Perform a Diagnostic Digest

    Type
    Protocol
    ...they recognize, restriction digests have become the most widely used method scientists employ to selectively...cut the plasmid into 3-8 pieces such that all (or most) fragments are small enough to be accurately sized...

  7. Centrifugation

    Type
    Protocol
    ...labels for the core parts that would be found on almost any centrifuge. Types and Parts There are several...techniques. Regardless of the type of centrifuge you use, most will have essentially the same components. The photo...

  8. Gibson Assembly Protocol

    Type
    Protocol
    ...Cloning? No need for specific restriction sites. Join almost any two fragments regardless of sequence. No scar...contiguous DNA fragment. Pro-Tip Add Extreme Thermostable Single-Stranded DNA-Binding protein (ET SSB)...

  9. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ...contains the plasmid DNA separated from bacterial chromosomes. Collect the supernatant into a new tube by pipetting.... This is included in the resuspension buffer of most kits. Reference Page Top Index...

  10. AAV Titration by qPCR Using SYBR Green Technology

    Type
    Protocol
    ...green are the ones loaded onto the qPCR plate for most samples. If sample is expected to have a titer <...have some small amount of background signal that is most evident during initial PCR cycles. This background...

  11. Pouring LB Agar Plates

    Type
    Protocol
    ...Although our recommended temperature should kill most potential contaminants, certain types of spores ... agar will remain liquid at this temperature but most antibiotics will not break down at this temperature...

  12. Kit Free RNA Extraction

    Type
    Protocol
    ...is. While simple homogenization is effective for most mammalian tissues; more hardy tissues such as bone...

  13. DNA Quantification

    Type
    Protocol
    ...different wavelengths of light to varying degrees and most have a specific wavelength that they maximally absorb...

  15. Water Bath Protocol

    Type
    Protocol
    ...and room temperature. This general protocol suits almost any water bath. Video Watch the video for tips ...
Showing: 1 - 20 of 22 results