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We narrowed to 902 results for: Nes

Showing: 461 - 480 of 902 results

  1. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    ...Easy to host guests remotely. Easy access from smartphones with low data requirements.  No visuals. Variety... audience interpretation. Infographics Low Combines both words and images; especially useful for conveying... to be staffed. Mixed Media Medium to high Combines pros of multiple channels/balance out cons. Flexible...in multiple ways from the same experience.  Can combines cons of multiple medias. Switching from one media...

  2. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    ...recurring journal clubs on Immunology and Microbial Pathogenesis that occurred once a week for several months...of the discussed topic, and level of general busy-ness will most likely the primary drivers of frequency...attendance slumps below a certain level or interest wanes off such that attendees are not actively participating... with any recurring meeting, a journal club’s usefulness should be continually evaluated and assessed ...

  3. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    ...Transgenic mouse lines expressing the 3x FLAG‐dC as9 protein for enCh IP analysis." Genes to cells 23.4 ...technology using recombinant CRISPR ribonucleoproteins." Genes to Cells 21.4 (2016): 370-377. PubMed PMID: 26848818...interactions between genomic regions by enCh IP‐Seq." Genes to Cells22.6 (2017): 506-520. PubMed PMID: 28474362...

  4. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ... and Cas13 systems in a range of mammalian cell lines. However, in those same comparisons, the AbuGoot...using Cas7-11. This included tests done in cell lines known to be more sensitive to toxicity from RNA ...activity. They now had a system that could integrate genes up to 36 kb in length, with an integration rate ...primary cells, and can be easily programmed for new genes. In comparison with other large insert techniques...

  5. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type
    Blog Post
    ...inspiration  I look back at the meeting with such fondness, knowing that, as a 38 year-old with many years... that only appear once) in our analysis. Many pipelines toss these singletons out, and there is healthy...Finding mentors for the meeting Just like analysis pipelines for DNA that group similar sequences together ...bacteria of different evolutionary lineages share genes that can improve chances of survival for an individual...

  6. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...with the development of methodologies to deliver genes to the CNS have recently made it much easier to ..., and the long-term expression of AAV delivered genes. AAV serotypes have been extensively studied in ...experiments with tag-specific antibodies. The most famous ones are influenza hemagglutinin (HA), myelocytomatosis...been used to visualize “thorny excrescence” (TE) spines in the Hippocampal CA3 pyramidal neurons which ...

  7. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    ...ice.synthego.com. We rigorously evaluated the effectiveness of the ICE tool by analyzing thousands of edits...performed over multiple experiments and comparing the robustness, accuracy, and speed with existing tools. For..., ICE is configured for Cas9 fromStreptococcus pyogenes (SpCas9). ICE Score - The editing efficiency (...green (edited sample) and orange (control sample) lines should be close together before the cut site. A ...

  8. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    ...become popular. There are only so many methods and machines one team can learn so linking together expert ...external connection is to make sure you keep open lines of communication with colleagues who move on to ...conference attendance, and I scout for potential business partners. I am glad that I was able to build experience...hallmark for jobs focusing on: Science Policy, Business Development, Administration and Intellectual Property...

  9. How to Write a Scientific Review Article

    Type
    Blog Post
    ... you have these rules (word limit, formatting guidelines, etc.) you have some criteria to shape the document...To find reviews, I just searched online and found ones that I thought “looked good” by no definitive criteria...This could be in the form of lists, streams of consciousness, or anything else. Like I mentioned, I also ...T-cell development, physiology and malignancy." Genes & cancer 6.5-6 (2015): 184. PubMed PMID: 26124919...

  10. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ...Thus, contour lines that are close together show a high density of cells, while contour lines that are spread...can also be presented as contour plots. Contour lines, which you may have seen on elevation maps, are ... of cells (Figure 7).  Figure 7: Contour lines in flow data from Jhunjhunwala et al., 2015. Used...

  12. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    ...to interfere with the function of resident ER chaperones and may impact cellular function or possibly ... blue or cyan and yellow moxFPs. The inherent brightness and spectral characteristics of the moxFPs are...negative bacteria periplasm. Efforts to tag endogenous genes for proteins in these compartments using CRISPR ...tagging to detect proteins expressed from cloned genes: deletion mapping functional domains of Drosophila...

  14. Ligation Independent Cloning

    Type
    Protocol
    ... and may provide counter-selection for negative clones as in the example shown here. The manufacturer ...

  15. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    ...demonstrate a POI’s absence. It also helps confirm that lanes are loaded evenly. Created with BioRender.com. ...blot. So how can you accurately compare different lanes? In relative quantification, the POI bands are normalized...blue) to infer or calculate things about earlier ones. In practice, multiple sources of error (red) influence...detection range of the assay is between the two dotted lines. Created with BioRender.com. To see why this...

  16. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    ...   Some phage display libraries screen antibody genes from B cells of immunized animals, but it's possible...screening scFv generated from the B cell antibody genes from diseased or vaccinated humans. How do you produce...cytoplasm. expression in the presence of molecular chaperones that help scFvs fold properly in the cytoplasm...also be fused to cellular toxins, radioisotopes, cytokines, and enzymes for cancer, autoimmune, and/or inflammatory...

  17. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    ...typical PCR machines, the authors use affordable, field-ready, Bluetooth-enabled PCR machines that can even...approximately 1-2 hours Equipment needed: Mini PCR machines by DIY-Bio, P51 cardboard fluorescence visualizer...mentioned, likely ~1-2 hours Equipment needed: PCR machines/Heat blocks, Lateral flow strips, Agarose gel ...

  18. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ...s group created Spinach2 using site-directed mutagenesis of Spinach. In doing so, they identified the ... intracellularly due to a lower dependency on magnesium and higher thermal stability, making it a potentially...and were presented in the study to support the uniqueness of the photostability of the Corn-DFHO complex...biotin. Two of these variants exhibit increased brightness compared to Mango, and all are photostable and...

  19. March for Science

    Type
    Blog Post
    ...functions. Experiments come next, testing the correctness of the hypothesis. The results of experiments...organisms (GMOs), the absence of a link between vaccines and autism, and other hypotheses mount yet policies...some non-political issues are split along party lines (18). Feeling like an apple will fall up instead...related to the following subjects: Climate change Vaccines GMOs Transgender issues Reproductive health Immigration...

  20. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    ...powerful site-specific knock-in tool that can be harnessed by researchers for many applications. Your favorite... chromosomal aberrations. Pfeiffer P, et al. Mutagenesis (2000). PubMed. Multiple Pathways of Recombination... Increased Efficiency of CRISPR/Cas-mediated Transgenesis (2018). Pubmed. The Road Less Traveled: Strategies... Increased Efficiency of CRISPR/Cas-mediated Transgenesis (2018). Pubmed. CRISPR References: Genome ...
Showing: 461 - 480 of 902 results