We narrowed to 566 results for: PAC

1. Top Requested Lentivirus and AAV of 2016

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   Blog Post

   ...These viruses have been distributed in over 200 packages to more than 20 countries. With this initial success...

2. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

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   ...violet-excited GFP variant Vex. To understand the impact of this gene editing tool on the immune system,...

3. Why and how to start studying chromatin (with Addgene's help)

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   ...of a nucleus as “linear” is a misnomer. The DNA packed into our chromosomes is very much 3-dimensional...

4. Targeted Mutagenesis with EvolvR

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   ...example the Phage Assisted Continuous Evolution (PACE) method requires constant turbidity of the culture...

5. Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors

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   ... General Auditor at Tufts Health Plan. In that capacity he leads the Internal Audit function and is responsible...

6. Early Career Researcher Toolbox: Social Media for Scientists

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   ...ve: 1) convinced you social media can positively impact your career, 2) given you tips for using various...

7. A Deep Dive into BioBuilder

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   ... opened in August 2017.  This flexible teaching space offers STEM programming, workshops, and customized...

8. Plasmids 101: Methylation and Restriction Enzymes

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   ... the adenine in AACNNNNNNGTGC or GCACNNNNNNGTT Impact on cloning and digests Although not all prokaryotic...

9. Hot Plasmids - May 2022

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   Blog Post

   ...believes that this system could potentially be packaged into an AAV system. dCas9-SSAP will make the editing...

10. Antibodies 101: Isotypes

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    ...structure, which means each IgM molecule has a binding capacity or valency of 10 antigens. An extra constant domain...

11. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

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    ...gain-of-function screening when combined with a compact pooled sgRNA library. Our SAM-mediated screens ...

12. Antibodies 101: Fab Fragments

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    ...sometimes you want a tool that is a little more compact — more of an antibody fragment rather than the ...

13. Antibodies 101: Conjugation

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    ...region or even just nearby, then conjugation could impact the antibody’s antigen recognition and affinity...

14. Plasmids 101: The protein expression toolbox

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    Blog Post

    ...choice cDNA. Both lentivirus and retrovirus can package cDNAs for genomic delivery and have many therapeutic...

15. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

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    ...two-postdoc households. To better assess the potential impact of these salary fluctuations, we are continuing...

16. Lighting Up Cell Signaling with Photoswitchable Kinases

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    ...signal strength, and how long their signals last all impact transduction of the message. To study signaling...

17. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

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    Blog Post

    ... the aforementioned methods only determine the capacity of homodimerization in aqueous solution. In cyto...

18. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

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    Blog Post

    ...eliminates the need for intracranial injections. When packaged in PHP.eB, ChRgers and ChR2(H134R) both reach ...

19. Summer SciComm Series: Cognitive Load

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    Blog Post

    ...communicate. It’s okay (and encouraged) to leave blank spaces or to gloss over some aspects when needed. The ...

20. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

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    Blog Post

    ... from these studies is that the tag protein can impact the expression of the transgene of interest. In...