We narrowed to 1,052 results for: STI

...mutations, we still weren’t entirely happy with our editing outcomes and in many cases still didn’t get ...must undergo homology-directed repair (HDR), a distinct cellular DNA repair pathway. Accomplishing this... be screening a total of a few hundred clones - Still better than 10.000, right? (Kwart, Paquet, et al...reads that we studied by deep sequencing, but interestingly (and to our frustration) in many cases we only...currently a Professor for Neurobiology at the Institute for Stroke and Dementia Research, Ludwig-Maximilians-University...

...cytosine-to-thymine substitutions in target sequences (Banno et al., 2018). These substitutions were limited ...enable C-to-T and A-to-G base substitutions respectively. Substitutions seem most efficient when using... recombineering took place. This system is distinct from those used in eukaryotes in that CRISPR isn...targeted and preferentially depleted from gut. While still far from the clinic, CRISPR-Cas3 systems have been... Sharp JD, Xu R, Prisic S, Husson RN (2016) Investigating essential gene function inMycobacterium tuberculosisusing...

...Even if we stick to citation counts for a moment, it’s clear that articles in less-prestigious journals ...proportion of highly cited articles appearing in lower-prestige journals has risen steadily from 1995 to 2013.... in citations for older publications. (As an interesting aside, Nature recently reported that the most...of another, perhaps it’s worth reflecting on a question of impact that only you can answer: How would ...

...Feng Zhang (of the Broad Institute of MIT and Harvard and the McGovern Institute for Brain Research) – and...I have an important question: How much coffee does it take to fuel more than 30,000 neuroscientists over...Though, to be fair, I think this is a conservative estimate. As a graduate student, I drank 3-4 cups of coffee...Zhang was awarded one of this year’s SfN Young Investigator Awards and we couldn’t wait to congratulate ...

...portable microscope. Less fluorescence admittedly, but still so beautiful! Enabling the scientist in everyone...buoyancy devices” (also known as gas vesicles) as acoustic biomolecule-based dyes. This technology is widely...about ‘Using ultrasound to image bacteria in vivo: acoustic reporter genes’ on our blog. Creative approaches...telling a story makes scientific topics both interesting and approachable!   The grand finale of visual.... References 1. Bourdeau, Raymond W., et al. "Acoustic reporter genes for noninvasive imaging of microorganisms...

... Human Dynamics Laboratory identified five characteristics of very successful teams. Check out Joanne'...if others are involved. They may feel they are wasting their time. The manager may appear to be too busy...to get people sharing.   Frequently ask direct questions for specific feedback in non-public settings. ...demonstrate patience, pause; A = ask at least one question to clarify the situation; C = confirm that you...employees who volunteer constructive feedback and suggestions. Consider offering rewards (gift card, afternoon...

... suffer any less acutely.  The Rare Genomics Institute works to help those who suffer from rare diseases...Through their BeHEARD Award, the Rare Genomics Institute partners with companies to provide technological...Elia Di Schiavi is a Research Scientist at the Institute of Biosciences and Bioresources in Naples, Italy...of spinal cord motor neurons. This disease is estimated to affect anywhere from 1 in 6,000 to 1 in 19,...prioritize which genes they will use to develop diagnostic tools and animals models of the disease in partnership...

...promoter regulating the gene of interest with a constitutive, robust M24 promoter. Originally isolated from...from the Mirabilis mosaic virus, the constitutively active M24 promoter has been modified with duplicated...this new vector, pSiM24, can also be used with biolistic methods, making it useful for engineering plants...large gene transfer. The M24 promoter provides constitutive, high-level gene expression and is functional...antibiotic resistance gene is controlled by a weaker constitutive Nos promoter, and it can be used to select both...

...in December were developed as an answer to that question. Additionally, a concurrent Nature Biotechnology... really not possible before,” said the Broad Institute's Ophir Shalem. “Whole regions of the genome which...delivery vehicle, Neville Sanjana, also of the Broad Institute, said they were able to produce a very diverse... of one of the Science papers, told the Broad Institute. “This will greatly aid efforts to understand ... RNA Library. Koike-Yusa H, Li Y, Tan E-P, Del Castillo Velasco-Herrera M, Yusa K. Nature Biotechnology...

...Kaustubh Kishor Jadhav, a Research Assistant at MGMs Institute of Biosciences and Technology. If you are reading...culturist has to deal with this problem. It is estimated that mycoplasma is responsible for up to 60% of...always use a second assay to be 100% sure. Before testing for mycoplasma, the cells should be in continuous... for low levels of contaminations to grow. For testing, the medium should not have been changed for at...regularly and dampness of any kind should be avoided. Distilled water provided to maintain the humidity should...Jadhav, MSc is a Research Assistant at the MGMs Institute of Biosciences and Technology in India. Jadhav...

...was in the lab, if you read a paper and saw an interesting construct you wished to use, you could either...we share? NanoLuc® Luciferase-based tools are fantastic examples of technologies that can accelerate your... fusions for imaging Fluorescent proteins are fantastic imaging tools but their use is limited in vivo... activation of other fluorescent probes but can still transfer energy to the fluorescent protein acceptor...create a calcium sensor with the following characteristics: No photoexcitation High Ion specificity High...Yang, J., et al. (2016) Coupling optogenetic stimulation with NanoLuc-based luminescence (BRET) Ca++ sensing...

...called polyhistidine tags, typically include six consecutive His residues (or more). Histidine bonds with...naturally occurring histidine-rich proteins in mammals. Mammalian purification is still possible but requires...cleaved off from the fusion protein while it is still bound to the glutathione resin, eliminating the ...

...Controlling Stimulation Some studies rely on delivering reversible or varied amounts of stimulation to receptors...are: Timing Targeted manipulation Controlling stimulation Invasiveness  Choosing the best set of tools...injected, this is not as precise and the offset of stimulation is gradual. Invasiveness Both optogenetic and...intracranial implant Noninvasive Controlling Stimulation ExogenousEasily controlled EndogenousNot easily...

...recurring meetings, you should share interesting results or ask questions to your collaborators. This helps...person responsible for incorporating everyone’s suggestions into a final version of the document. Set and...to specialized microscopes and allowed me to investigate my protein of interest in a French clinical cohort...’re looking to improve communication within an existing collaboration, or start something new! Additional...

...number of extinct transposon sequences and then testing various combinations of changes. The basic functional...transposase system - one set of vectors for robust constitutive expression (pSBbi derivatives) and one set of...inducible expression (pSBtet derivatives). The constitutive and inducible expression vectors were developed...16 variants with different selection markers (blasticidin, neomycin, puromycin, hygromycin) and fluorescent...robustly express your gene-of-interest, either constitutively or regulated by doxycycline. The Sleeping Beauty...

...pGP-AAV-syn-FLEX-jGCaMP7b-WPRE) exhibits the brightest resting fluorescence of the GCaMP7 family of calcium sensors...respond to blue light. We recently released a constitutive (pAAV-Syn-Archon1-KGC-GFP-ER2) and cre-dependent...next 6 months, pending quality control. For an estimate on availability date or to be notified when a ...exhibits high contrast between peak fluorescence and resting fluorescence. It is useful for activity imaging...exhibits high contrast between peak fluorescence and resting fluorescence.  It is useful for activity imaging...

...experimental system comes with fewer ethical and logistical challenges than does working with animals. Because...staff from the ABRC are here to support you! From questions about growing conditions, to guiding students ... are available via e-mail and phone to answer questions and provide support. Growing capacity - ABRC outreach...science learning. Whether visiting classrooms, hosting field trips, conducting teacher workshops, or staffing... staffing a booth at local science festivals, ABRC gets the word out about the importance of plant sciences...

...people, you will find the answer to your viability question. The miLab you see today is far different than... was viable.  This is not to say that we aren’t still pitching for feedback.  Your venture should continuously...partnerships with universities, companies, hospital institutions and funding bodies. Don't shy away from the ...Although many emails resulted in no response, I was still able to get through to a number of experts, including...make sure you’re able to answer every possible question related to it. First impressions are everything...

...of the population as a whole, scientists gain statistical power in their observations. However, while flow... of FSC versus SSC that allows them to observe distinct cell populations and rule out dead cells and debris...complexity, these cell types will separate into distinct populations on a FSC versus SSC plot (Figure 2...flow cytometry experiment and will appear as a distinct population. After initially gating the lymphocytes...marker) to separate the B cell and T cell into distinct populations (Figure 2). An immunologist who studies...cells from a whole blood sample will separate into distinct populations of monocytes, lymphocytes and granulocytes...

...sure it is working? This article addresses these questions, which are critical in setting up a FRET-based... the wrong address in a different city, you can still develop a successful FRET-based assay since energy...interest: you just don’t know it yet! The poly-histidine tag often used for protein purification is a reliable...dye, as well as by extending the length of the histidine tag from 6 to 10 residues. While these Cy/NTA ...incorporation of fluorescent probes into protein: hexahistidine-tag-mediated fluorescent labeling with (Ni2+...FRET-Based Approach for Antibody Detection–A New Serodiagnostic Concept." PloS one 8.5 (2013): e62739. PubMed...