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1. What's New in CRISPR - November 2021

   Type

   Blog Post

   ...read more content related to CRISPR technology, subscribe to our CRISPR blog content.   In this post we ...

2. Antibodies 101: Polyclonal Antibodies

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   Blog Post

   ...protein G each have different affinities for IgG subtypes from different animals so it’s important to choose...

3. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

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   Blog Post

   ...the same cell, but not necessarily at the same subcellular compartment as the opsin or chemogenetic receptor...

4. Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute

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   Blog Post

   ...Twitter @sejkeown. If you enjoyed this series, subscribe to the Career Channel of the blog so you don’t...

5. Celebrating 15 Years of Scientific Sharing

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   Blog Post

   ...', style='' %} Resources on the Addgene blog Subscribe to the Addgene blog Read our viral vector blog...

6. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

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   Blog Post

   ...them to keep raising salaries and that we are submitting Freedom of Information Act (FOIA) requests for...

7. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

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   Blog Post

   ...offer your comments and suggestions, and even to submit a guest blog entry of your own! References 1. ...

8. Gendered Innovations: Why Does Sex of the Cell Matter?

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   Blog Post

   ... research, they worry about needing more study subjects and control groups. The important point, however...

9. Addgene's Tips for Plasmid Quality Control

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   Blog Post

   ...to select the appropriate service and read the submission instructions. As for the analysis of a large ...

10. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

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    Blog Post

    ... Slaymaker et al. made a variety of alanine substitutions throughout the groove in 32 separate Cas9 mutants...

11. New Tool for Lineage Tracing: The ClonTracer Library

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    Blog Post

    ...cells that represent the preexisting resistant subpopulation 2. Resistant cells among the separate replicates...

12. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

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    Blog Post

    ...toolboxes and making them available. We’ll also be submitting parts from another paper for resource allocation...

13. Career Coaching for Scientists: Why and Where Do I Find One?

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    Blog Post

    ...Additional Resources on the Addgene Blog 6 Steps to Submitting a Resume That Gets Seen  Guide Your PhD with ...

14. A Look at Addgene's QC Process

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    Blog Post

    ...can affect experimental processes, results, and subsequent conclusions. Being vigilant about these errors...

15. Antibodies 101: Stripping and Reprobing Western Blots

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    Blog Post

    ...whether that involves adding fresh chemiluminescent substrate or imaging your fluorescent probe. Then, if you...

16. Plasmids 101: Modular Cloning

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    Blog Post

    ...constructs capable of performing production and subsequent necessary modification of these chemical products...

17. A Guide to Designing a Scientific Poster: Content, Layout, and More

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    Blog Post

    ...want to add a bit more “flash,” you can include a subtle gradient (as is shown in all of our illustrations...

18. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

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    Blog Post

    ...alters neuronal function directly such as receptor subunits or neurotrophic factors. Another option uses optogentics...

19. Mouse Modeling, Part 1: Genetically Engineered Mice

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    Blog Post

    ...conditional systems Conditional knockouts or knockins A subset of transgenic mice, conditional knockout/knockin...

20. Engaging with science and society at pgEd

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    Blog Post

    ...science-outreach organizations or student publications, submitting op-eds to your local newspapers, or starting ...