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...To overcome this obstacle, a positive selection cassette can be incorporated, and used during the differentiation..., but defective for integration [4], the donor cassette is removed leaving behind no accessory transposon...recombination and Footprint-Free™ removal of the selection cassette by excision-only piggyBac™. Image courtesy of ...recombinase technology (Cre/loxP) to remove the donor cassette. While this method can be effective, a loxP site..., it is possible to make the ITR and selection cassette disrupt the target site for the nuclease thereby...preventing re-cutting. Transposase removal of the cassette later restores the target sequence. This allows...

...resource for spatial biology enthusiasts. This dataset currently includes 1049 community-validated reagents...into conversations with other users. The entire dataset can be downloaded from Zenodo, including the reagent...table or by downloading directly from the Zenodo dataset.     Figure 2: Screenshot showing reagent...HuBMAP) antibody metadata standards. And the dataset is rich. Experimental data includes reagent type...protocols, panels, publications, software, and datasets. It’s also a great place for anyone who has used...

... Biological General Repository for Interaction Datasets (BioGRID) in order to increase the accessibility...accessibility of CRISPR-based phenotypic screen datasets. Original published CRISPR screen data is curated and ...and ready access to stably archived large-scale datasets by computational biologists. Figure 1: ...other species. By accurately compiling genome-wide datasets, which are often obscured in large supplementary...but also allows cross-interrogation of different datasets that might otherwise be hard to identify. ...

...bacterial selection cassette for growth and cloning in bacteria and a mammalian selection cassette to screen for...cells, you will not need a mammalian selection cassette (e.g. 1435 pSG5L Flag HA). You can also upgrade...with an integrating vector expressing a selection cassette with 5′ and 3′ flanking sequences matching the...fusion proteins. Pick your promoter, selection cassette and visualization vehicle to conduct your custom...

...Producing yeast expression cassettes Traditionally, generating expression cassettes for yeast transformation...-fidelity polymerase) is the ability to remove, reset and ligate mismatched overhangs, yielding scarless...Cloning sgRNAs Production of Yeast Expression Cassettes Generating constructs with multiple point mutations...regions of homology and inserted into a linearized cassette, ready for transformation into yeast that same...

...resistance cassette and you aren’t introducing an additional antibiotic resistance cassette along with...plasmid backbone contains a kanamycin resistance cassette, you would plate the cells on kanamycin-containing...carrying plasmids that contain a kanamycin resistance cassette will grow. However, often times, the plasmid backbone...

... Scientists introduce an antibiotic resistance cassette within the coding region of the gene they are ...is best practice not to use the same resistance cassette for the mutation and for plasmid selection. Additionally... vector typically has two separate resistance cassettes and your gene of interest is cloned into/inactivates...completely removes (in the case of Gateway cloning) one cassette. Counterselection allows the scientist to select...

... background. Their new method uses a selection cassette containing genes for hygromycin resistance and...heat-shock inducible Cre to generate a self-excising cassette (SEC). To make it possible fluorescently label...into the genome. Figure 1: Self-excising cassette schematic from Dickinson et al. (2015). Used with...Engineering with a Self-Excising Drug Selection Cassette. Dickinson DJ, Pani AM, Heppert JK, Higgins CD...

...strewn across the floor. In the final wake it all settles down and the value of each item changes from what...individual; the  community as a whole has yet to settle on any true kind of value. I’ve dubbed this the...software design that is applicable to only one, or a subset of organisms. In only a few cases the software ... agnostic. Most tools are exclusive to one or a subset of organisms. Examples: “organism agnostic”, “model... you can rule out any tool that limits you to a subset of specific genomes. These are most of the tools...

...available metagenomic datasets with biophysical studies and the potential of mining datasets for research and...Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids...metagenomic data including the Tara Oceans Project datasets. This study demonstrates the power of combining...

...targeting the genes responsible for both early onset and late onset AD. In this study, the group showed that...connected to the early onset of Alzheimer’s disease, as well as those that play into late onset AD (5).     ...

...version that fits your setup: Plasmid Backbone Promoter pRSET OCaMP pRSET T7 pAAV-hSYN1...compatible with the lasers available in your microscope setup. Figure 1: Excitation (dotted) and emission...

...aTet-inducible gRNA and a backbone-targeting gRNA cassette for plasmid curing through "self-destruction" ... while pCRISPomyces-2 contains Cas9 and a gRNA cassette. The simpler system of pCRISPomyces-2 displays...). By cloning in two tandem copies of the gRNA cassette, one can reliably knockdown expression of multiple...such a purpose (Selle et al., 2020).  Targeting a subset of bacteria in a population Work from the Lu lab...found early success using CRISPR-Cas to target subsets of bacteria in population based on the presence...

... of a stop cassette upstream of the target gene in the targeting vector. The stop cassette is floxed, ...conditional systems Conditional knockouts or knockins A subset of transgenic mice, conditional knockout/knockin...so Cre-mediated recombination deletes the stop cassette, which permits expression of the target gene. ...

...unbiased data. Therefore, we chose to examine a dataset of Liu lab plasmid requests from Addgene, as a ... requests correlate with paper publication The dataset includes 11,426 plasmid requests over 6 years for...and 03/11/20 -- 05/19/20 (the last date in our dataset) for the U.S. and Europe. To improve the stability...China. An important additional caveat is that our dataset contains plasmid orders from just one lab, which...

... frequently used elements in plant expression cassettes: plant promoters, N-terminal tags, coding sequences...C-terminal tags, plant terminators and resistance cassettes for selection of transgenic plants. The complete... gives the option to stack several expression cassettes onto a single T-DNA in subsequent rounds, dramatically...

...gain fresh perspectives. Such activities foster a mindset that embraces the unexpected and encourages exploration...Serendipity is not just a stroke of luck; it's a mindset that can lead to unexpected discoveries and innovative...serendipity into your research but also cultivate a mindset that is adaptable, creative, and poised for groundbreaking...

.... In the specific example of episodic memory, a subset of hippocampal neurons projects axons directly ...tracing has the added benefit of only labelling the subset of infected cells that also express Cre. Another...AAV vectors. Conclusion An ideal comprehensive toolset for neuronal tracing and network identification...adeno-associated viral vector serotypes 1, 2, 5, 8 and 9 in marmoset, mouse and macaque cerebral cortex, Neurosci Res...

...re-administration but I do not think the field has settled on ‘best practices’ for model organisms.    Q11...expression is when injecting CAP-B10 or CAP-B22 in marmosets, from an individual to another individual perspective...CAP-B10 and CAP-B22 were tested individually in 4 marmosets each. Brain potency for each individual is reported...

...et al   Multiplexed Lentiviral Expression Cassettes lentiviral transduction One vector expresses... own promoter   Kabadi et al Csy4-Cleavable Cassettes transfection gRNAs expressed from a polycistronic...co-transfection of Cas9 Nissim et alTsai et al PTG Cassettes transfection gRNAs expressed from a polycistronic...