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Antibodies 101: Buffers, Storage, and Conjugates

Type

Blog Post

... the buffer, which will be noted on the product sheet. Thankfully, sodium azide can easily be removed ...
Antibodies 101: Isotypes

Type

Blog Post

...Antibodies 101: Introduction to Antibodies for a refresher). There are five antibody isotypes that each have...
Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

Type

Blog Post

...posts Find more about fluorescent proteins Get a refresher on CRISPR with our CRISPR 101 blog posts Resources...
Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

Type

Blog Post

... from this collection in your experiments and published your data, please fill out this form and we’ll...
Developing a cold tolerant E. coli using specialized chaperones

Type

Blog Post

...survive, but thrive, in the face of some of Earth’s harshest environments: corrosive pH ranges, crushing pressures...
Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

Type

Blog Post

...of endogenous signaling activity in cells. Let’s shed some light on the key components of OptoNBs, how...
Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

Type

Blog Post

...In January 2016 we first published a blog post titled: Which Cas9 Do I Choose for My CRISPR Experiment...
CasPEDIA: A Functional Classification of Cas Enzymes

Type

Blog Post

... includes biochemical properties, previously established uses, and intended applications. Within CasPEDIA...
GCE4All: Making Genetic Code Expansion Accessible

Type

Blog Post

...the mid-aughts, the first yeast GCE paper was published, followed soon by GCE done in mammalian cells ...
Viral Vectors 101: Viruses as Biological Tools

Type

Blog Post

...specific. Once inside the host cell, the virus unleashes its cargo – its own genome. Fig. 1:...
How Addgene Creates Content

Type

Blog Post

... the example of our Antibody Guide, which was published alongside our new antibody services in the spring...
Viral Vectors 101: Gamma-Retroviral Packaging Systems

Type

Blog Post

...updated by multiple Addgenies. It was originally published in 2018, in the Viral Vectors 101 eBook, 1st edition...
15 Years of Addgene: The Top 15 Plasmids

Type

Blog Post

...lentiviral) or pUMVC (MuLV retroviral). Fun fact: published in 2003, this plasmid predates Addgene’s founding...
Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

Type

Blog Post

...attention on transcription factors, starting with an established method called ChIP-seq. ChIP-seq relies on antibodies...
Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

Type

Blog Post

...Hijacking tRNA processing to make gRNAs In a recently published paper, Yinong Yang’s group did just that as part...
How to Make Friends and Meet People at a Scientific Conference

Type

Blog Post

...Congratulate your connections when they get a new paper published. Stay in touch enough to plan to meet up at the...
Kiran Musunuru on the Newest TALEN Genome-Editing System

Type

Blog Post

...moment you are designing a TALEN on paper to a finished plasmid. With our protocol, it is feasible to ...
Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

Type

Blog Post

...purpose. Tang wondered if he could use previously established mouse lines expressing GFP in specific cell types...
Lighting Up Cell Signaling with Photoswitchable Kinases

Type

Blog Post

.... Zhou, Xin X., Linlin Z. Fan, Pengpeng Li, Kang Shen, and Michael Z. Lin. "Optical control of cell signaling...
Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

Type

Blog Post

...Oligomerization Seeing Red: Simple GFP Photoconversion Light Sheet Fluorescence Microscopy Additional Resources on...

Showing: 461 - 480 of 595 results

Results per page:

Antibodies 101: Buffers, Storage, and Conjugates

Antibodies 101: Isotypes

Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

Developing a cold tolerant E. coli using specialized chaperones

Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

CasPEDIA: A Functional Classification of Cas Enzymes

GCE4All: Making Genetic Code Expansion Accessible

Viral Vectors 101: Viruses as Biological Tools

How Addgene Creates Content

Viral Vectors 101: Gamma-Retroviral Packaging Systems

15 Years of Addgene: The Top 15 Plasmids

Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

How to Make Friends and Meet People at a Scientific Conference

Kiran Musunuru on the Newest TALEN Genome-Editing System

Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

Lighting Up Cell Signaling with Photoswitchable Kinases

Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

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