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Showing: 461 - 480 of 556 results

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Plasmids 101: Gram Negative and Positive Bacteria

Type

Blog Post

.... & Walker S. The Bacterial Cell Envelope. Cold Spring Harb Perspect Biol. 2(5) a000414 (2010). Cleveland...
REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

Type

Blog Post

...determine if a variant is causal for a given disease. CRISPR has made genomic SDM relatively straightforward...
Hot Plasmids - May 2022

Type

Blog Post

...efficiency of up to 20%, comparable to commonly used CRISPR/Cas9 methods for large inserts. However, this system...
Sweating the Small Stuff: Details in the Lab

Type

Blog Post

.... If you’ve got messy handwriting, reach for spreadsheets, printouts, and even label makers to keep things...
Improved Plasmid Maps Powered by SnapGene

Type

Blog Post

...are commonly used for Golden Gate cloning or for CRISPR gRNA cloning. With this more functional display...
A Practical Approach to Choosing the B(right)est Fluorescent Protein

Type

Blog Post

... In mammalian cells this would be possible with CRISPR/Cas-based genome editing. Selecting a bright fluorescent...
GCE4All: Making Genetic Code Expansion Accessible

Type

Blog Post

...more posts on the various protocols and helping to spread the word as new protocols come out! References...
Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

Type

Blog Post

... therapeutics, and other treatments to stem the spread of COVID-19. That’s why we’re proud to be working...
Developing a cold tolerant E. coli using specialized chaperones

Type

Blog Post

...temperature conditions, thereby enabling efficient CRISPR-Cas mediated editing for psychrophilic systems ...
Viral Vectors 101: Gamma-Retroviral Packaging Systems

Type

Blog Post

...The widespread use of γ-retroviruses (gamma-retroviruses) in cancer and stem cell research has prompted...
A Novice's Perspective on Neuroscience - SfN2017

Type

Blog Post

...interactions with Alzheimer's, I found others using CRISPR to generate new knockout mice, using adenoviral...
3 Challenges in Plant Synthetic Biology

Type

Blog Post

...engineering technology. Monoculture and chemical spraying, mainstays of modern agriculture, are now synonymous...
Developing Lab Management Software for Biology

Type

Blog Post

...where you learn biology terms (plasmid, 5-prime, CRISPR, growth strains, vector backbone, etc.) and you...
Antibodies 101: Polyclonal Antibodies

Type

Blog Post

... (2019) Antibody Purification and Storage. Cold Spring Harb Protoc 2019:pdb.top099101. https://doi.org...
All in a Twist: dsRNA

Type

Blog Post

...021-00430-1 More resources on the Addgene blog CRISPR 101: RNA Editing with Cas13 RNA Interference ...
RNA Extraction Without A Kit

Type

Blog Post

...RNA. Keep a clean work area, which may include spraying your bench down with a product to get rid of RNases...
Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

Type

Blog Post

...Blog Read the Plasmid 101 blog posts Check out CRISPR Meets Synthetic Biology: A Conversation with MIT...
Visualizing Protein Turnover In Situ

Type

Blog Post

...translation in living single cells. However, before CRISPR-Cas9 these techniques were quite tedious and, even...
5 Tips for Troubleshooting Viral Transductions

Type

Blog Post

...adoptive immunotherapy." Acute Leukemias VIII. Springer Berlin Heidelberg, 2001. 291-306. PubMed PMID:...
Tips for Titering Your Lentiviral Preps

Type

Blog Post

... Lentivirus FAQ Learn How Lentivirus is Used in CRISPR Genome-Wide Screening Learn How Lentivirus Has ...

Showing: 461 - 480 of 556 results

Results per page:

Plasmids 101: Gram Negative and Positive Bacteria

REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

Hot Plasmids - May 2022

Sweating the Small Stuff: Details in the Lab

Improved Plasmid Maps Powered by SnapGene

A Practical Approach to Choosing the B(right)est Fluorescent Protein

GCE4All: Making Genetic Code Expansion Accessible

Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

Developing a cold tolerant E. coli using specialized chaperones

Viral Vectors 101: Gamma-Retroviral Packaging Systems

A Novice's Perspective on Neuroscience - SfN2017

3 Challenges in Plant Synthetic Biology

Developing Lab Management Software for Biology

Antibodies 101: Polyclonal Antibodies

All in a Twist: dsRNA

RNA Extraction Without A Kit

Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

Visualizing Protein Turnover In Situ

5 Tips for Troubleshooting Viral Transductions

Tips for Titering Your Lentiviral Preps

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