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We narrowed to 13 results for: Spr

Showing: 1 - 13 of 13 results

  1. CRISPR Guide

    Type
    Guide
    ...Guide CRISPR Guide Jump To: CRISPR Guide Overview CRISPR Basics Small Edits Large Edits Genome-Wide Screening...increase nuclease activity SpRY - NRN/NYN, where R = A or G, Y = C or T SpRYc - SpRY PAMs plus NNG; a chimeric...Figure 7: Overview of CRISPR transposases Browse Plasmids: CRISPR transposases CRISPR Recombinases and Integrases...screening using CRISPR libraries What Are Pooled Lentiviral CRISPR Libraries? Pooled lentiviral CRISPR libraries...transcriptional repression (CRISPR interference, or CRISPRi) or activation (CRISPRa) of downstream target genes...References Website CRISPR plasmids and resources at Addgene CRISPR 101 eBook (3rd edition) CRISPR software and... Addgene Blog CRISPR topic page How to design your gRNA for CRISPR genome editing CRISPR 101: Homology...

  2. Plan Your Experiment

    Type
    Guide
    ... Science Guides CRISPR Guide Plan Your Experiment Plan Your CRISPR Experiment Jump To: Plan ...information on the wide range of CRISPR technologies, visit our CRISPR Guide . There are many different...framework to get you started using CRISPR in your research and setting up CRISPR experiments. We will focus on...transcription elongation. CRISPR Activation : Target the transcription start site. CRISPRa requires dCas9 to ...systems utilized for CRISPR/Cas gene editing. This image summarizes ways that the CRISPR/Cas system can be...Learn how to plan a basic CRISPR experiment, including selecting the edit type, designing and cloning...Expression and Delivery Validate Genetic Edit References CRISPR ( C lustered R egularly I nterspaced S hort P alindromic...

  3. Guide to Using Pooled Libraries

    Type
    Guide
    ...Using CRISPR/Cas9 Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening Pooled CRISPR Libraries...protocol. Read our CRISPR guide for more information on genome-wide screening with CRISPR/Cas9 . Browse our...of published selection mechanisms for lentiviral CRISPR libraries include: resistance to the anti-cancer...virus, make virus; this creates a pooled lentiviral CRISPR library. Apply pooled library to cells. DIVERGE... plasmids have been depleted by selection. In a CRISPR screen, negative screens are often used to identify...

  4. Addgene's eBook Collection

    Type
    Guide
    ...Close About Overlay Select CRISPR 101 January 2021 | Third Edition About CRISPR 101 January 2021 | ...Overlay Select CRISPR 101 en español January 2024 | First Edition, trans. About CRISPR 101 en español...experimental advice such as tips for planning your CRISPR experiment and designing your gRNA and how to use...traducción al español de nuestro libro electrónico, CRISPR 101. Close About Overlay Select Antibodies 101 ...

  5. Science Guides

    Type
    Guide
    ...Read More CRISPR Class 2 C lustered R egularly I nterspaced S hort P alindromic R epeat (CRISPR) systems...background information on CRISPR and the basics for planning your first CRISPR experiment. Read More Optogenetics...links to the guides below. Antibodies Chemogenetics CRISPR Optogenetics Pooled Libraries Viral Vectors Antibodies...

  6. Lentiviral Vector Guide

    Type
    Guide
    ...Addgene's collection of in-stock lentiviral preps . CRISPR Genomic Screens Genome-wide screens are an efficient...Addgene has an extensive collection of lentiviral CRISPR libraries, including knockout, inhibition/activation...and base editing. Browse Addgene's collection of CRISPR libraries . Cellular Barcoding Much like genomic...Popular uses include Cre-lox mice, knockouts using CRISPR or shRNA , and overexpressing of oncogenes and ...Doench, J. G., & Zhang, F. (2013). Genome-Scale CRISPR-CAS9 knockout screening in human cells. Science...

  7. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ... Addgene. CRISPR/Cas9 Delivery rAAV vectors are a popular choice for the delivery of CRISPR/Cas9 elements...size, low immunogenicity, and limited ability to spread after injection. Different AAV serotypes exhibit...making it one of the most common methods for in vivo CRISPR-based genome editing. Packaging the Cas9 from Streptococcus...blog post on overcoming AAV size limitation for CRISPR delivery for more information. Split AAVs to increase...

  8. Molecular Biology Reference

    Type
    Guide
    ...editing is most commonly accomplished using CRISPR technology. CRISPR is composed of a DNA endonuclease and ...the genome. For more information on CRISPR check out Addgene’s CRISPR guide . Reporter Plasmids - Used for...

  9. Educational Resources

    Type
    Guide
    ...to our collections. Titles include: Plasmids 101 CRISPR 101 Viral Vectors 101 Fluorescent Proteins 101 ...include: Genome Engineering Fluorescent Proteins CRISPR Viral Vectors Plasmid Technology Career Readiness...in-depth instructional content on subjects such as: CRISPR Chemogenetics Optogenetics Genetic Code Expansion...

  10. Chemogenetics Guide

    Type
    Guide
    ...different AAV serotypes have varying degrees of viral spread within injected tissue, and this must be considered...especially useful in AAV experiments where the virus can spread around the injection site and potentially express...

  11. Adenovirus Guide

    Type
    Guide
    ...of time or when needing to package larger cargo. CRISPR-Cas9 Delivery As compared to other viral vectors...Production and Troubleshooting Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells...

  12. Optogenetics Guide

    Type
    Guide
    ...tools for optical control of neural activity. Cold Spring Harb Protoc. 3:top102. PMID 21363959 Yizhar O, ...

  13. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...Retroviral Vector Design. In Retroviruses . Cold Spring Harbor Laboratory Press. NIH Bookshelf De Ravin...
Showing: 1 - 13 of 13 results