We narrowed to 562 results for: Spr;

1. Developing a cold tolerant E. coli using specialized chaperones

   Type

   Blog Post

   ...temperature conditions, thereby enabling efficient CRISPR-Cas mediated editing for psychrophilic systems ...

2. A Novice's Perspective on Neuroscience - SfN2017

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   Blog Post

   ...interactions with Alzheimer's, I found others using CRISPR to generate new knockout mice, using adenoviral...

3. Hot Plasmids - May 2022

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   Blog Post

   ...efficiency of up to 20%, comparable to commonly used CRISPR/Cas9 methods for large inserts. However, this system...

4. Sweating the Small Stuff: Details in the Lab

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   Blog Post

   .... If you’ve got messy handwriting, reach for spreadsheets, printouts, and even label makers to keep things...

5. Improved Plasmid Maps Powered by SnapGene

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   Blog Post

   ...are commonly used for Golden Gate cloning or for CRISPR gRNA cloning. With this more functional display...

6. A Practical Approach to Choosing the B(right)est Fluorescent Protein

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   Blog Post

   ... In mammalian cells this would be possible with CRISPR/Cas-based genome editing. Selecting a bright fluorescent...

7. GCE4All: Making Genetic Code Expansion Accessible

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   Blog Post

   ...more posts on the various protocols and helping to spread the word as new protocols come out! References...

8. Viral Vectors 101: Gamma-Retroviral Packaging Systems

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   Blog Post

   ...The widespread use of γ-retroviruses (gamma-retroviruses) in cancer and stem cell research has prompted...

9. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

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   Blog Post

   ...determine if a variant is causal for a given disease. CRISPR has made genomic SDM relatively straightforward...

10. 3 Challenges in Plant Synthetic Biology

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    Blog Post

    ...engineering technology. Monoculture and chemical spraying, mainstays of modern agriculture, are now synonymous...

11. RNA Extraction Without A Kit

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    Blog Post

    ...RNA. Keep a clean work area, which may include spraying your bench down with a product to get rid of RNases...

12. Visualizing Protein Turnover In Situ

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    Blog Post

    ...translation in living single cells. However, before CRISPR-Cas9 these techniques were quite tedious and, even...

13. Antibodies 101: Polyclonal Antibodies

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    Blog Post

    ... (2019) Antibody Purification and Storage. Cold Spring Harb Protoc 2019:pdb.top099101. https://doi.org...

14. Developing Lab Management Software for Biology

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    Blog Post

    ...where you learn biology terms (plasmid, 5-prime, CRISPR, growth strains, vector backbone, etc.) and you...

15. All in a Twist: dsRNA

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    Blog Post

    ...021-00430-1 More resources on the Addgene blog CRISPR 101: RNA Editing with Cas13  RNA Interference ...

16. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

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    Blog Post

    ...Blog Read the Plasmid 101 blog posts Check out CRISPR Meets Synthetic Biology: A Conversation with MIT...

17. Meet an Addgenie: Insights into Grad School, Career Paths, and More

    Type

    Blog Post

    ...Perkins presented a poster a few years ago at a CRISPR meeting.Read his Instagram feature here. You...

18. Overcoming the Challenges of Lentiviral Production

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    Blog Post

    ...for transfer plasmids with small inserts (such as CRISPR guides) while a single 72h harvest is best for ...

19. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

    Type

    Blog Post

    ...testing in the lab will be greatly facilitated by CRISPR reagents and other specific gene sequences available...

20. Adenoviral Vector Production and Troubleshooting

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    Blog Post

    ...- a primer for beginners Adenoviral delivery of CRISPR/Cas9 5 tips for troubleshooting viral transductions...