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Showing: 461 - 480 of 562 results

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Droplet Digital PCR for AAV Quantitation

Type

Blog Post

...to contamination. Optimize your PCR For a good starting place on what PCR parameters to use, see Lock’...
Getting to Know Expansion Microscopy

Type

Blog Post

...post-expansion, but that doesn’t mean we should start re-conceptualizing actin filaments or microtubules...
CRISPR 101: Making a Knock-In Cell Line

Type

Blog Post

... edits of small insertions and deletions. Start your knock-in experiment today! Get ready to identify...
Negative Can Be Positive: Open AAV Data with Addgene

Type

Blog Post

...neuroscience Let’s consider my first challenge when I started working with AAV vectors. I wanted to use an AAV...
CRISPR Antimicrobials

Type

Blog Post

...slow. CRISPR may be just the method needed to jumpstart the fight against antibiotic resistance. Advantages...
Cpf1: A New Tool for CRISPR Genome Editing

Type

Blog Post

...cleavage and G-rich PAM requirement. Zetsche et al. started from square one to characterize the Cpf1 nucleases...
Negotiating Work and Life: How to Find the Joy

Type

Blog Post

...going away to a conference. Really. No one will starve while you are away a few days. Also, your children...
Deciphering the Mysteries of Behavior with Viral Vectors

Type

Blog Post

...viral vectors blog posts Find tips for getting started with viral vectors for behavioral neuroscience ...
Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

Type

Blog Post

...role as a receptor for PHP.B. The Deverman lab started by seeing how changes in LY6A’s expression affected...
What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

Type

Blog Post

...Now what? What would you study first? They are starting by seeing if they can change long legged lizards...
Designing Your Chalk Talk for the Academic Job Interview

Type

Blog Post

...you as a colleague and give you a million dollar startup package. There are three steps to chalk talk preparation...
Neuronal labeling with Spaghetti Monster

Type

Blog Post

...of intelligent design in public schools and was started in 2005 in Kansas by Bobby Henderson, a physics...
Analyzing CRISPR Editing Results with ICE from Synthego

Type

Blog Post

...NGS-based amplicon sequencing. To use the tool, you start by uploading your Sanger sequencing files, either...
Plasmids 101: Gateway Cloning

Type

Blog Post

...expression elements (ribosome recognition sequences, start codon, stop codons, reading frame considerations...
To Codon Optimize or Not: That is the Question

Type

Blog Post

... you may want to codon optimize before you get started. There are a ton of online resources available ...
Plasmids 101: Plasmid Incompatibility

Type

Blog Post

...same replicon and/or partitioning system. Let’s start with the replicon- the part of the plasmid that ...
Antibodies 101: Reading a Flow Plot

Type

Blog Post

...FACS plot and follow a gating strategy, you can start to ask why the researchers made the decisions they...
CRISPR 101: Any Base Transversion Editors

Type

Blog Post

...Tong, Wang, et al., 2023). Based on this promising start, the team used rational mutagenesis to produce the...
New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

Type

Blog Post

...days. GVs should be visible under phase contrast starting day 2 after transfection. Fig: 2: Expressing...
CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

Type

Blog Post

...demonstrated that targeting dCas9 to transcriptional start sites was sufficient to repress transcription by...

Showing: 461 - 480 of 562 results

Results per page:

Droplet Digital PCR for AAV Quantitation

Getting to Know Expansion Microscopy

CRISPR 101: Making a Knock-In Cell Line

Negative Can Be Positive: Open AAV Data with Addgene

CRISPR Antimicrobials

Cpf1: A New Tool for CRISPR Genome Editing

Negotiating Work and Life: How to Find the Joy

Deciphering the Mysteries of Behavior with Viral Vectors

Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

Designing Your Chalk Talk for the Academic Job Interview

Neuronal labeling with Spaghetti Monster

Analyzing CRISPR Editing Results with ICE from Synthego

Plasmids 101: Gateway Cloning

To Codon Optimize or Not: That is the Question

Plasmids 101: Plasmid Incompatibility

Antibodies 101: Reading a Flow Plot

CRISPR 101: Any Base Transversion Editors

New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

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