We narrowed to 617 results for: TIL
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TypeBlog Post...supernatant migrates through increasingly dense layers until the macromolecule density is equal to the buoyant...
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CasPEDIA: A Functional Classification of Cas Enzymes
TypeBlog Post... However, many situations could arise where you still need more information. If you are performing a viral... -
Viral Vectors 101: Inverted Terminal Repeats
TypeBlog Post... efficiency. Our practical knowledge of ITRs is still limited, but this area of research is important ... -
Viral Vectors 101: Viral Vector Elements
TypeBlog Post...into the host genome (more on this below), it’s still used to deliver gene expression constructs. Sandwiched... -
Viral Vectors 101: Biosensors
TypeBlog Post...you covered with viral resources and safety tips. Still worried? We make virus with some of our most popular... -
Viral Vectors 101: Viral Applications
TypeBlog Post...Additional Resources on the Addgene Blog AAV: A Versatile Viral tool for Gene Expression in Mammals Listen... -
Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering
TypeBlog Post...similar cleavage products even when guides were tiled across a target RNA, indicating that this enzyme... -
Plasmids 101: Methylation and Restriction Enzymes
TypeBlog Post...from the restriction modification systems, yet can still affect whether certain restriction enzymes can effectively... -
How to Keep a Lab Notebook for Bioinformatic Analyses
TypeBlog Post...commands entered is useful for beginners who are still learning the basics. However, these notes take up... -
Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens
TypeBlog Post...in my opinion, had not been achieved.” That is, until now. Enter the SAM pooled library In a paper published... -
How to Make Friends and Meet People at a Scientific Conference
TypeBlog Post...conference, but start with a more general intro until you know if the person you are talking to is really... -
3 Challenges in Plant Synthetic Biology
TypeBlog Post...over 30 years ago, but plant synthetic biology is still in its infancy. A long timeline from transformation... -
Seven Tips for Using LinkedIn as a Scientist
TypeBlog Post... manager. This is considered intrusive and it’s still connecting with a stranger so is highly discouraged... -
Lighting Up Cell Signaling with Photoswitchable Kinases
TypeBlog Post...Raf-MEK-ERK signaling pathway to demonstrate the utility of photoswitchable kinases in vivo with C. elegans... -
Choosing the B(right)est Fluorescent Protein: Aggregation Tendency
TypeBlog Post...cells (Shaner et al, 2013; Bindels et al, 2017). Still, the data presented in papers should be used with... -
Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics
TypeBlog Post...stages of development, ensuring the breadth and versatility of this tool. Find CreLite at Addgene. Yen et... -
Plasmids 101: CcdB - The Toxic Key to Efficient Cloning
TypeBlog Post... (re-ligated empty vectors, for instance) would still express ccdB and therefore would die. This procedure... -
Summer SciComm: Preprints
TypeBlog Post...directly alongside the preprint while the work is still in progress or awaiting a final publication in a... -
Antibodies 101: Affinity Reagents
TypeBlog Post...trendy as that may sound. Validated antibodies are still an excellent option for many applications, and frankly... -
Visualizing Protein Turnover In Situ
TypeBlog Post...Germany. She presented a novel technology, back then still unpublished, that allowed the visualization of specific...