We narrowed to 931 results for: TIM

...conditions, cells must be able to detect extracellular stimuli. One way they do so is through G protein-coupled...engineering and experimental refinement to determine the optimal localization of the Rluc8 insertion within the ...refinement, it was not possible to generate an optimal Rluc8-Gα construct for two of the 16 non-visual...

...so that you can spend less time researching the basics and spend more time developing cleverly designed... Gateway, Gibson, etc). The cloning method is ultimately chosen based on the plasmid you want to clone...

...methods missing something? Here we will review some time-tested gene identification systems that may have...expression levels (high/low) and patterns (tissue/timing) can be monitored under various conditions without...require the trapping of endogenous enhancers to stimulate the expression of the reporter. The system is ...

...19% integration in HEK293T cells (more than 500 times better than the precursor it was evolved from!) ...to specific mutations require individualized optimizations, safety testing, and approval. In contrast, ...higher efficiency, but evoCAST required less optimization for new targets and produced a higher-purity...

...more likely) impossible to find; therefore all the time spent collecting it will have been worthless. But...ASM CEO Stefano Bertuzzi welcoming us. Then it was time to work! Set up similarly to the online class, we...technique, then type up the materials and methods. Over time that portion of the paper will be generated. After...

...helped grad students who were teaching for the first time. I walk[ed them] through their lecture, giving them...interests. “So for me, I researched G-proteins at the time. This program takes what you know about it [your... I was. It was really luck of right place, right time, and having a good interview experience.” Although...

...fresh insights and broaden your understanding, ultimately enhancing your project's potential. Widening ...mindful meditation, taking field trips, and spending time outdoors in nature or visiting museums can be excellent...ideas. It's beneficial to carry a notebook at all times (or use the notes app on your phone) to capture ...

...Check out Joanne's Reddit AMA  Few of us spend much time thinking about conserving resources in the labs....wasteful packaging.  Turns out, one could make a lifetime supply for about $10 US dollars worth of glass... our protocol for making competent cells to save time and money. My other stingy science project started...

...whether an advisor will be a good mentor: Spend time with the potential advisor and don’t be shy about...lab, if possible. Attend a lab meeting. Spend some time hanging out with members of the lab. It is a warning...if the PI does not welcome you to visit and spend time with everyone. Additionally, if you are looking...

...undergraduate degree (and I hope I’m not alone in this sentiment). Entering the final semester of a neuroscience...findings of brain science, my curiosity did not ultimately translate to a desire to do the research itself...percolate within a new intellectual environment. Over time, this interest came to incorporate other facets ...

...communication research.   This summer, we’re making time to talk about my favorite thing - science communication...knowledge than average and are more likely to spend time engaging with science communication efforts.  Scientific...about science communication itself! See you next time!  Additional resources on the Addgene blog 10 ...

...something Stegmeier has been interested in for a long time. “At an early age, I was fascinated by the impact...therapy.” He’s also worked on vaccines throughout that time. Now Vandenberghe is an Associate Professor at Mass... walls we’ve built create inefficiencies that ultimately make innovation and science slow,” Vandenberghe...

...antibodies that have almost certainly already been optimized for your application. Depending on the tag, directly...of epitope tags exist – all with individual optimizations and specific monoclonal antibodies. Below we... acid tag (DYKDDDDK) that can be added multiple times (often 3X FLAG, but can be more!) to a protein’s...

...Science Magazine. Although we only walk one path at a time, the variety of paths in life is mind-boggling. ...aspects of bioscience into context, while at the same time working through the details of experimental design...discoveries into an entertaining podcast? Look at our multimedia producer and online editor. And, the copyeditors...

...this; however, you should reduce your incubation time on ice to 5 minutes (incubating the full 30 minutes...group! You may want to include extra extension time after the last cycle of PCR to make sure that the... is not recommended that you exceed the 5 minute time limit (lower transformation efficiencies have been...

...launched in the early spring of 2015 and since that time have received about 3 project proposals per month...making connections and finding mentors -- well, it's time you call upon these people for guidance.  Before... evolve and respond to feedback throughout its lifetime.  In this way, you will always improve and give...

...University of Cambridge and knew it was time to leave. I really enjoyed my time in the lab, my colleagues and what...choices were mostly by chance. Luckily, around the time I decided to look for work outside of academia, ...

... job. EAs shouldn't be viewed as an unproductive time spent away from the bench, but as an opportunity... conferences and seminars, as this is a valuable time to network Attend informational interviews to ...required qualifications and skill sets Taking the time to identify and research 2 – 3 potential professions...

... DAP array, short for “drive-and-process.” To optimize DAP use, they developed a generalizable method...sequences must be avoided, different tRNAs with optimized 5’ leader sequences can be used together in DAP... Reporters to Monitor and Enrich Editing in Real Time Prime Editing: Adding Precision and Flexibility ...

...iGluSnFR and SF-iGluSnFR have made it clear that it’s time to update our iGluSnFR post! Here, we look at the...generations of iGluSnFR had limitations: they were not optimal for experiments requiring high signal to noise ...Tools References Marvin, Jonathan S., et al. “An optimized fluorescent probe for visualizing glutamate neurotransmission...